Thu, 21 Aug 2008 01:45:39 BST CiteULike: Author Dmitri http://www.citeulike.org/author/Dmitri CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/Pezda/article/2804397 <i>Medical Microbiology and Immunology, Vol. 197, No. 2. (June 2008), pp. 241-249.</i><br /><br />The promise of the mouse model of cytomegalovirus (CMV) research lies in a cost effective way to obtain significant data in in vivo settings. Keeping that promise requires a high degree of equivalency in the human and mouse virus. While genomic structure and many common proteins suggest that this system is appropriate to develop and test concepts in an organismal context, areas of difference have not been evaluated. Here we show that the major immediate early protein 1 (IE1) in MCMV binds the repressor Daxx suggesting that it serves a function performed by pp71 in HCMV. A Daxx binding pp71 equivalent at M82 could not be identified for MCMV. Differences in the mouse and human interferon upregulation of Daxx may have driven the need to have a Daxx-defeating function during reactivation, when pp71 is not present. The major immediate early protein 1 also differs in its chromatin binding properties between the two viruses. MCMV IE1 does not bind to chromatin, but HCMV IE1 does. It remains unclear whether this difference is functionally significant. The HCMV major immediate early protein 2 and its MCMV equivalent IE3 differ in their effect on the cell cycle; HCMV IE2 blocks the cell cycle, but MCMV IE3 does not, allowing MCMV to spread in infected mouse cells by cell division with continued expression of the major transactivating viral proteins. Actively transcribing genomes inducing immediate transcript environments are usually silenced and diminish during cell cycle progression. However, a recognizable desilencing and increase in immediate transcript environments takes place immediately after mitosis in MCMV infected cells. This raises the possibility that desilencing happens during tissue transplantation, wound healing, or other injury where cells are induced to proliferate. Differences between mouse and human cytomegalovirus interactions with their respective hosts at immediate early times of the replication cycle Gerd Maul Dmitri Negorev doi:10.1007/s00430-008-0078-1 Medical Microbiology and Immunology, Vol. 197, No. 2. (June 2008), pp. 241-249. 2008-05-16T05:57:41-00:00 2008 Medical Microbiology and Immunology 0300-8584 197 2 241 249 Springer hcmv immediate-early mcmv http://www.citeulike.org/user/jyuh/article/1287340 <i>Bioinformatics, Vol. 23, No. 8. (15 April 2007), pp. 1032-1034.</i> ClusterDraw web server: a tool to identify and visualize clusters of binding motifs for transcription factors Papatsenko Dmitri doi:10.1093/bioinformatics/btm047 Bioinformatics, Vol. 23, No. 8. (15 April 2007), pp. 1032-1034. 2007-05-10T04:35:50-00:00 2007 Bioinformatics 1367-4803 23 8 1032 1034 Oxford University Press no-tag http://www.citeulike.org/user/jpassoth/article/1742855 <i>Sociological Theory, Vol. 25, No. 3. (September 2007), pp. 193-224.</i> Signing in the Flesh: Notes on Pragmatist Hermeneutics Shalin N Dmitri doi:10.1111/j.1467-9558.2007.00305.x Sociological Theory, Vol. 25, No. 3. (September 2007), pp. 193-224. 2007-10-08T17:49:15-00:00 2007 Sociological Theory 0735-2751 25 3 193 224 Blackwell Publishing hermeneutics pragmatism social-theory