Thu, 21 Aug 2008 10:09:19 BST CiteULike: Author Robic http://www.citeulike.org/author/Robic CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/msuarezdiez/article/1197806 <i>Proc Natl Acad Sci U S A, Vol. 100, No. 20. (30 September 2003), pp. 11345-11349.</i><br /><br />Ribonucleases H from the thermophilic bacterium Thermus thermophilus and the mesophile Escherichia coli demonstrate a dramatic and surprising difference in their change in heat capacity upon unfolding (DeltaCp degrees ). The lower DeltaCp degrees of the thermophilic protein directly contributes to its higher thermal denaturation temperature (Tm). We propose that this DeltaCp degrees difference originates from residual structure in the unfolded state of the thermophilic protein; we verify this hypothesis by using a mutagenic approach. Residual structure in the unfolded state may provide a mechanism for balancing a high Tm with the optimal thermodynamic stability for a protein's function. Structure in the unfolded state is shown to differentially affect the thermodynamic profiles of thermophilic and mesophilic proteins. Role of residual structure in the unfolded state of a thermophilic protein. S Robic M Guzman-Casado JM Sanchez-Ruiz S Marqusee doi:10.1073/pnas.1635051100 Proc Natl Acad Sci U S A, Vol. 100, No. 20. (30 September 2003), pp. 11345-11349. 2007-03-30T11:22:51-00:00 2003 Proc Natl Acad Sci U S A 0027-8424 100 20 11345 11349 protein_design unfolded_state http://www.citeulike.org/user/dchen/article/2767637 <i>Physical Review Letters, Vol. 100, No. 10. (2008)</i><br /><br />We introduce a general methodology based on magnetic colloids to study the recognition kinetics of tethered biomolecules. Access to the full kinetics of the reaction is provided by an explicit measure of the time evolution of the reactant densities. Binding between a single ligand and its complementary receptor is here limited by the colloidal rotational diffusion. It occurs within a binding distance that can be extracted by a reaction-diffusion theory that properly accounts for the rotational Brownian dynamics. Our reaction geometry allows us to probe a large diversity of bioadhesive molecules and tethers, thus providing a quantitative guidance for designing more efficient reactive biomimetic surfaces, as required for diagnostic, therapeutic, and tissue engineering techniques. Measuring the Kinetics of Biomolecular Recognition with Magnetic Colloids Cohen Tannoudji E Bertrand J Baudry C Robic C Goubault M Pellissier A Johner F Thalmann Lee CM Marques J Bibette doi:10.1103/PhysRevLett.100.108301 Physical Review Letters, Vol. 100, No. 10. (2008) 2008-05-07T23:01:32-00:00 2008 Physical Review Letters 100 10 APS 2008 colloids magnetic http://www.citeulike.org/user/Ayest/article/1064687 <i>Mamm Genome, Vol. 14, No. 1. (January 2003), pp. 71-80.</i><br /><br />On porcine Chromosome 7, the region surrounding the MHC region contains QTL influencing many traits including growth, back fat thickness, and carcass composition. Towards the identification of the responsible gene(s), this article describes an increase of density of the radiated hybrid map of SSC 7 in the q11-q14 region and the comparative analysis of gene order on the porcine RH map and human genome assembly. Adding 24 new genes in this region, we were able to build a framework map that fills in gaps on the previous maps. The new software Carthagene was used to build a robust framework in this region. Comparative analysis of human and porcine maps revealed a global conservation of gene order and of distances between genes. A rearranged fragment of around 3.7 Mb was, however, found in the pig approximately 20 Mb upstream from the expected location on the basis of the human map. This rearrangement, found by RH mapping on the IMpRH 7.000 rads panel, has been confirmed by two-color FISH and by mapping on the high resolution IMNpRH2 12.000 rads panel. The rearranged fragment contains two microsatellites found at the most likely QTL location in the INRA QTL experiment. It also contains the BMP5 gene, which, together with CLPS, could be considered as a possible candidate. Rearranged gene order between pig and human in a QTL region on SSC 7. O Demeure C Renard M Yerle T Faraut J Riquet A Robic T Schiex A Rink D Milan doi:10.1007/s00335-002-3034-1 Mamm Genome, Vol. 14, No. 1. (January 2003), pp. 71-80. 2007-01-24T08:54:13-00:00 2003 Mamm Genome 0938-8990 14 1 71 80 lgc map porc http://www.citeulike.org/user/Ayest/article/1064643 <i>Cytogenet Genome Res, Vol. 102, No. 1-4. (2003), pp. 100-108.</i><br /><br />In this study we examined homologies between 1,735 porcine microsatellites and human sequence. For 1,710 microsatellites we directly used the sequence flanking the repeat available in GenBank. For a set of 305 microsatellites, a BAC library was screened and end-sequencing provided 461 additional sequences. Altogether 2,171 porcine sequences were tentatively aligned with the sequence of the human genome using the fasta program. Human homologies were observed for 652 microsatellite loci and porcine chromosome assignments available for 623 microsatellites provide useful links in the human and pig comparative map. Moreover for 92 STS, a significant sequence similarity was detected using at least two sequences and in all cases corresponding human locations were consistent. The present study allowed the integration of anonymous markers and the porcine linkage map into the framework of the comparative data between human and porcine genomes (http://w3.toulouse.inra.fr/lgc/pig/msat/). Moreover all conserved syntenic segments were defined on human chromosomes. A new contribution to the integration of human and porcine genome maps: 623 new points of homology. A Robic T Faraut N Iannuccelli Y Lahbib-Mansais V Cantegrel L Alexander D Milan doi:10.1159/000075733 Cytogenet Genome Res, Vol. 102, No. 1-4. (2003), pp. 100-108. 2007-01-24T08:51:01-00:00 2003 Cytogenet Genome Res 1424-859X 102 1-4 100 108 lgc map porc