Thu, 21 Aug 2008 15:49:47 BST CiteULike: Malaria Journal - Latest articles http://www.citeulike.org/journal/malariajournal CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/article/3134832 <i>Malaria Journal, Vol. 7 (18 August 2008), 157.</i> Phagocytosis of haemozoin (malarial pigment) enhances metalloproteinase-9 activity in human adherent monocytes: Role of IL-1beta and 15-HETE Mauro Prato Valentina Gallo Giuliana Giribaldi Paolo Arese doi:10.1186/1475-2875-7-157 Malaria Journal, Vol. 7 (18 August 2008), 157. 2008-08-19T02:31:25-00:00 2008 Malaria Journal 1475-2875 7 157 http://www.citeulike.org/article/3134831 <i>Malaria Journal, Vol. 7 (18 August 2008), 156.</i> Dry season ecology of Anopheles gambiae complex mosquitoes in The Gambia Musa Jawara Margaret Pinder Chris Drakeley Davis Nwakanma Ebrima Jallow Claus Bogh Steve Lindsay David Conway doi:10.1186/1475-2875-7-156 Malaria Journal, Vol. 7 (18 August 2008), 156. 2008-08-19T02:31:25-00:00 2008 Malaria Journal 1475-2875 7 156 http://www.citeulike.org/article/3129081 <i>Malaria Journal, Vol. 7, No. 1. (2008)</i><br /><br />BACKGROUND:Antibodies targeting variant antigens expressed on the surface of Plasmodium falciparum infected erythrocytes have been associated with protection from clinical malaria. The precise target for these antibodies is unknown. The best characterized and most likely target is the erythrocyte surface-expressed variant protein family Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1). METHODS:Using recombinant proteins corresponding to five domains of the expressed A4 var gene, A4 PfEMP1, the naturally occurring antibody response was assessed, by ELISA, to each domain in serum samples obtained from individuals resident in two communities of differing malaria transmission intensity on the Kenyan coast. Using flow cytometry, the correlation in individual responses to each domain with responses to intact A4-infected erythrocytes expressing A4 PfEMP1 on their surface as well as responses to two alternative parasite clones and one clinical isolate was assessed..RESULTS:Marked variability in the prevalence of responses between each domain and between each transmission area was observed, as wasa strong correlation between age and reactivity with some but not all domains. Individual responses to each domain varied strikingly, with some individuals showing reactivity to all domains and others with no reactivity to any, this was apparent at all age groups. Evidence for possible cross-reactivity in responses to the domain DBL4gamma was found.CONCLUSIONS:Individuals acquire antibodies to surface expressed domains of a highly variant protein. The finding of potential cross-reactivity in responses to one of these domains is an important initial finding in the consideration of potential vaccine targets. Acquisition of naturally occurring antibody responses to recombinant protein domains of Plasmodium falciparum erythrocyte membrane protein 1 Claire Mackintosh Zoe Christodoulou Tabitha Mwangi Moses Kortok Robert Pinches Thomas Williams Kevin Marsh Christopher Newbold doi:10.1186/1475-2875-7-155 Malaria Journal, Vol. 7, No. 1. (2008) 2008-08-16T21:57:16-00:00 2008 Malaria Journal 1475-2875 7 1 http://www.citeulike.org/article/3104960 <i>Malaria Journal, Vol. 7 (09 August 2008), 154.</i> Different methodological approaches to the assessment of in vivo efficacy of three artemisinin-based combination antimalarial treatments for the treatment of uncomplicated falciparum malaria in African children Elizabeth Ashley Loretxu Pinoges Eleanor Turyakira Grant Dorsey Francesco Checchi Hasifa Burkirwa Ingrid van den Broek Issaka Zongo Pedro Michel van Herp Suna Balkan Walter Taylor Piero Olliaro Jean-Paul Guthmann doi:10.1186/1475-2875-7-154 Malaria Journal, Vol. 7 (09 August 2008), 154. 2008-08-10T02:19:16-00:00 2008 Malaria Journal 1475-2875 7 154 http://www.citeulike.org/article/3100838 <i>Malaria Journal, Vol. 7 (07 August 2008), 153.</i> Gains in awareness, ownership and use of insecticide-treated nets in Nigeria, Senegal, Uganda and Zambia Carol Baume Celeste Marin doi:10.1186/1475-2875-7-153 Malaria Journal, Vol. 7 (07 August 2008), 153. 2008-08-08T13:58:48-00:00 2008 Malaria Journal 1475-2875 7 153 http://www.citeulike.org/article/3100837 <i>Malaria Journal, Vol. 7 (07 August 2008), 152.</i> Ethnobotanical study of some of mosquito repellent plants in north-eastern Tanzania Eliningaya Kweka Franklin Mosha Asanterabi Lowassa Aneth Mahande Jovin Kitau Johnson Matowo Michael Mahande Charles Massenga Filemon Tenu Emmanuel Feston Ester Lyatuu Michael Mboya Rajabu Mndeme Grace Chuwa Emmanuel Temu doi:10.1186/1475-2875-7-152 Malaria Journal, Vol. 7 (07 August 2008), 152. 2008-08-08T13:58:48-00:00 2008 Malaria Journal 1475-2875 7 152 http://www.citeulike.org/article/3085657 <i>Malaria Journal, Vol. 7 (04 August 2008), 151.</i> Impact of urban agriculture on malaria vectors in Accra, Ghana Eveline Klinkenberg PJ Mccall Michael Wilson Felix Amerasinghe Martin Donnelly doi:10.1186/1475-2875-7-151 Malaria Journal, Vol. 7 (04 August 2008), 151. 2008-08-05T13:57:25-00:00 2008 Malaria Journal 1475-2875 7 151 http://www.citeulike.org/article/3075906 <i>Malaria Journal, Vol. 7 (02 August 2008), 150.</i> Consanguineous marriages and endemic malaria: can inbreeding increase population fitness? Srdjan Denic Nico Nagelkerke Mukesh Agarwal doi:10.1186/1475-2875-7-150 Malaria Journal, Vol. 7 (02 August 2008), 150. 2008-08-02T13:55:22-00:00 2008 Malaria Journal 1475-2875 7 150 http://www.citeulike.org/article/3075862 <i>Malaria Journal, Vol. 7, No. 1. (2008)</i><br /><br />BACKGROUND:Malaria is a very important cause of anaemia in tropical countries. Anaemia is assessed either by measurement of the haematocrit or the haemoglobin concentration. For comparisons across studies, it is often necessary to derive one measure from the other. METHODS:Data on patients with slide-confirmed uncomplicated falciparum malaria were pooled from 85 antimalarial drug trials conducted in 25 different countries, to assess the haemoglobin/haematocrit relationship at different time points in malaria. Using a linear random effects model, a conversion equation for haematocrit was derived based on 3,254 measurements from various time points (ranging from day 0 to day 63) from 1,810 patients with simultaneous measurements of both parameters. Haemoglobin was also estimated from haematocrit with the commonly used threefold conversion.RESULTS:A good fit was obtained using Haematocrit = 5.62 + 2.60 * Haemoglobin. On average, haematocrit/3 levels were slightly higher than haemoglobin measurements with a mean difference (+/-SD) of -0.69 (+/-1.3) for children under the age of 5 (n=1,440 measurements from 449 patients). CONCLUSIONS:Based on this large data set, an accurate and robust conversion factor both in acute malaria and in convalescence was obtained. The commonly used threefold conversion is also valid. The relationship between the haemoglobin concentration and the haematocrit in Plasmodium falciparum malaria Sue Lee Kasia Stepniewska Nicholas Anstey Elizabeth Ashley Karen Barnes Tran Binh Umberto D'Alessandro Nicholas Day Peter de Vries Grant Dorsey Jean Guthmann Mayfong Mayxay Paul Newton Francois Nosten Piero Olliaro Lyda Osario Loretxu Pinoges Ric Price Mark Rowland Frank Smithuis Robert Taylor Nicholas White doi:10.1186/1475-2875-7-149 Malaria Journal, Vol. 7, No. 1. (2008) 2008-08-02T12:56:42-00:00 2008 Malaria Journal 7 1 http://www.citeulike.org/article/3075905 <i>Malaria Journal, Vol. 7 (02 August 2008), 148.</i> Malaria morbidity in Papua Indonesia, an area with multidrug resistant Plasmodium vivax and Plasmodium falciparum Muhammed Karyana Lenny Burdarm Shunmay Yeung Enny Kenangalem Noah Wariker Rilia Maristela Ketut Umana Ram Vemuri Maurits Okoseray Pasi Penttinen Peter Ebsworth Paulus Sugiarto Nicholas Anstey Emiliana Tjitra Richard Price doi:10.1186/1475-2875-7-148 Malaria Journal, Vol. 7 (02 August 2008), 148. 2008-08-02T13:55:21-00:00 2008 Malaria Journal 1475-2875 7 148 http://www.citeulike.org/article/3075904 <i>Malaria Journal, Vol. 7 (01 August 2008), 147.</i> A quantitative risk assessment approach for mosquito-borne diseases: malaria re-emergence in southern France Nicolas Poncon Annelise Tran Celine Toty Adrian Luty Didier Fontenille doi:10.1186/1475-2875-7-147 Malaria Journal, Vol. 7 (01 August 2008), 147. 2008-08-02T13:55:21-00:00 2008 Malaria Journal 1475-2875 7 147 http://www.citeulike.org/article/3069452 <i>Malaria Journal, Vol. 7 (31 July 2008), 146.</i> Malaria vector control practices in an irrigated rice agro-ecosystem in central Kenya and implications for malaria control Peter Ng'ang'a Josephat Shililu Gayathri Jayasinghe Violet Kimani Charity Kabutha Lucy Kabuage Ephantus Kabiru John Githure Clifford Mutero doi:10.1186/1475-2875-7-146 Malaria Journal, Vol. 7 (31 July 2008), 146. 2008-08-01T01:49:32-00:00 2008 Malaria Journal 1475-2875 7 146 http://www.citeulike.org/article/3069451 <i>Malaria Journal, Vol. 7 (30 July 2008), 145.</i> Utilization of insecticide-treated nets by under-five children in Nigeria: Assessing progress towards the Abuja targets Olusola Oresanya Moshe Hoshen Olayemi Sofola doi:10.1186/1475-2875-7-145 Malaria Journal, Vol. 7 (30 July 2008), 145. 2008-08-01T01:49:32-00:00 2008 Malaria Journal 1475-2875 7 145 http://www.citeulike.org/article/3069450 <i>Malaria Journal, Vol. 7 (30 July 2008), 144.</i> Evaluation of the genetic polymorphism of Plasmodium falciparum P126 protein (SERA or SERP) and its influence on naturally acquired specific antibody responses in malaria-infected individuals living in the Brazilian Amazon Lilian Pratt-Riccio Selma Sallenave-Sales Joseli de Oliveira-Ferreira Bruno da Silva Monick Guimaraes Fatima Santos Thatiane De Simone Mariza Morgado Salvatore De Simone Maria Claudio Daniel-Ribeiro Mariano Zalis Daniel Camus Dalma Banic doi:10.1186/1475-2875-7-144 Malaria Journal, Vol. 7 (30 July 2008), 144. 2008-08-01T01:49:31-00:00 2008 Malaria Journal 1475-2875 7 144 http://www.citeulike.org/article/3063415 <i>Malaria Journal, Vol. 7 (29 July 2008), 143.</i> Early home treatment of childhood fevers with ineffective antimalarials is deleterious in the outcome of severe malaria Adebola Orimadegun Olukemi Amodu Peter Olumese Olayemi Omotade doi:10.1186/1475-2875-7-143 Malaria Journal, Vol. 7 (29 July 2008), 143. 2008-07-30T13:47:11-00:00 2008 Malaria Journal 1475-2875 7 143 http://www.citeulike.org/article/3063414 <i>Malaria Journal, Vol. 7 (29 July 2008), 142.</i> Cohort study of the association of antibody levels to AMA1, MSP119, MSP3 and GLURP with protection from clinical malaria in Ghanaian children Daniel Dodoo Anastasia Aikins Kwadwo Kusi Helena Lamptey Ed Remarque Paul Milligan Samuel Bosomprah Roma Chilengi Yaa Osei Bartholomew Akanmori Michael Theisen doi:10.1186/1475-2875-7-142 Malaria Journal, Vol. 7 (29 July 2008), 142. 2008-07-30T13:47:11-00:00 2008 Malaria Journal 1475-2875 7 142 http://www.citeulike.org/article/3046140 <i>Malaria Journal, Vol. 7 (26 July 2008), 141.</i> A physiological time analysis of the duration of the gonotrophic cycle of Anopheles pseudopunctipennis and its implications for malaria transmission in Bolivia Frederic Lardeux Rosenka Tejerina Vicente Quispe Tamara Chavez doi:10.1186/1475-2875-7-141 Malaria Journal, Vol. 7 (26 July 2008), 141. 2008-07-27T13:44:18-00:00 2008 Malaria Journal 1475-2875 7 141 http://www.citeulike.org/article/3044040 <i>Malaria Journal, Vol. 7 (24 July 2008), 140.</i> Epochal changes in the association between malaria epidemics and El Nino in Sri Lanka Lareef Zubair Gawrie Galappaththy Hyemin Yang Janaki Chandimala Zeenas Yahiya Priyanie Amerasinghe Neil Ward Stephen Connor doi:10.1186/1475-2875-7-140 Malaria Journal, Vol. 7 (24 July 2008), 140. 2008-07-26T01:41:54-00:00 2008 Malaria Journal 1475-2875 7 140 http://www.citeulike.org/article/3039575 <i>Malaria Journal, Vol. 7 (24 July 2008), 139.</i> Establishment of the 1st World Health Organization International Standard for Plasmodium falciparum DNA for nucleic acid amplification technique (NAT)-based assays David Padley Alan Heath Colin Sutherland Peter Chiodini Sally Baylis doi:10.1186/1475-2875-7-139 Malaria Journal, Vol. 7 (24 July 2008), 139. 2008-07-24T13:28:44-00:00 2008 Malaria Journal 1475-2875 7 139 http://www.citeulike.org/article/3039574 <i>Malaria Journal, Vol. 7 (23 July 2008), 138.</i> Efficacy of local neem extracts for sustainable malaria vector control in an African village Rebecca Gianotti Arne Bomblies Mustafa Dafalla Ibrahim Issa-Arzika Jean-Bernard Duchemin Elfatih Eltahir doi:10.1186/1475-2875-7-138 Malaria Journal, Vol. 7 (23 July 2008), 138. 2008-07-24T13:28:44-00:00 2008 Malaria Journal 1475-2875 7 138 http://www.citeulike.org/article/3039573 <i>Malaria Journal, Vol. 7 (23 July 2008), 137.</i> Plasmodium falciparum variant STEVOR antigens are expressed in merozoites and possibly associated with erythrocyte invasion Ayman Khattab Insa Bonow Nadine Schreiber Michaela Petter Christel Schmetz Mo-Quen Klinkert doi:10.1186/1475-2875-7-137 Malaria Journal, Vol. 7 (23 July 2008), 137. 2008-07-24T13:28:44-00:00 2008 Malaria Journal 1475-2875 7 137 http://www.citeulike.org/article/3039572 <i>Malaria Journal, Vol. 7 (23 July 2008), 136.</i> Dynamics of transmission of Plasmodium falciparum by Anopheles arabiensis and the molecular forms M and S of Anopheles gambiae in Dielmo, Senegal Mamadou Ndiath Cecile Brengues Lassana Konate Cheikh Sokhna Christian Boudin Jean Trape Didier Fontenille doi:10.1186/1475-2875-7-136 Malaria Journal, Vol. 7 (23 July 2008), 136. 2008-07-24T13:28:44-00:00 2008 Malaria Journal 1475-2875 7 136 http://www.citeulike.org/article/3035577 <i>Malaria Journal, Vol. 7 (22 July 2008), 135.</i> Prospects, achievements, challenges and opportunities for scaling-up malaria chemoprevention in pregnancy in Tanzania: the perspective of national level officers Godfrey Mubyazi Ib Bygbjerg Pascal Magnussen Oystein Olsen Jens Byskov Kristian Hansen Paul Bloch doi:10.1186/1475-2875-7-135 Malaria Journal, Vol. 7 (22 July 2008), 135. 2008-07-23T01:28:06-00:00 2008 Malaria Journal 1475-2875 7 135 http://www.citeulike.org/article/3035576 <i>Malaria Journal, Vol. 7 (22 July 2008), 134.</i> The impact of HIV-1 on the malaria parasite biomass in adults in sub-Saharan Africa contributes to the emergence of antimalarial drug resistance Jean-Pierre Van Geertruyden Joris Menten Robert Colebunders Eline Korenromp Umberto D'Alessandro doi:10.1186/1475-2875-7-134 Malaria Journal, Vol. 7 (22 July 2008), 134. 2008-07-23T01:28:05-00:00 2008 Malaria Journal 1475-2875 7 134 http://www.citeulike.org/article/3024319 <i>Malaria Journal, Vol. 7 (21 July 2008), 133.</i> Malaria in pregnant women in an area with sustained high coverage of insecticide-treated bed nets Abdunoor Kabanywanyi John Macarthur Wilma Stolk Dik Hassan Mshinda Peter Bloland Salim Abdulla Patrick Kachur doi:10.1186/1475-2875-7-133 Malaria Journal, Vol. 7 (21 July 2008), 133. 2008-07-21T13:27:02-00:00 2008 Malaria Journal 1475-2875 7 133 http://www.citeulike.org/article/3011572 <i>Malaria Journal, Vol. 7 (16 July 2008), 132.</i> Presumptive treatment of fever cases as malaria: help or hindrance for malaria control? Roly Gosling Christopher Drakeley Alex Mwita Daniel Chandramohan doi:10.1186/1475-2875-7-132 Malaria Journal, Vol. 7 (16 July 2008), 132. 2008-07-17T01:22:57-00:00 2008 Malaria Journal 1475-2875 7 132 http://www.citeulike.org/article/3011571 <i>Malaria Journal, Vol. 7 (15 July 2008), 131.</i> Competency of Anopheles stephensi mysorensis strain for Plasmodium vivax and the role of inhibitory carbohydrates to block its sporogonic cycle Hamid Basseri Soghra Doosti Kamran Akbarzadeh Mehdai Nategpour Miranda Whitten Hossein Ladoni doi:10.1186/1475-2875-7-131 Malaria Journal, Vol. 7 (15 July 2008), 131. 2008-07-17T01:22:56-00:00 2008 Malaria Journal 1475-2875 7 131 http://www.citeulike.org/article/3003170 <i>Malaria Journal, Vol. 7, No. 1. (2008)</i><br /><br />BACKGROUND:Molecular markers, particularly those associated with drug resistance, are important surveillance tools that can inform policy choice. People infected with falciparum malaria often contain several genetically-distinct clones of the parasite; genotyping the patients' blood reveals whether or not the marker is present (i.e. its prevalence), but does not reveal its frequency. For example a person with four malaria clones may contain both mutant and wildtype forms of a marker but it is not possible to distinguish the relative frequencies of the mutant and wildtypes i.e. 1:3, 2:2 or 3:1.METHODS:An appropriate method for obtaining frequencies from prevalence data is by Maximum Likelihood analysis. A computer programme has been developed that allows the frequency of markers, and haplotypes defined by up to three codons, to be estimated from blood phenotype data. RESULTS:The programme has been fully documented [see Additional File 1] and provided with a user-friendly interface suitable for large scale analyses. It returns accurate frequencies and 95% confidence intervals from simulated dataset sets and has been extensively tested on field data sets.CONCLUSIONS:The programme is included [see Additional File 2] and/or may be freely downloaded from [1]. It can then be used to extract molecular marker and haplotype frequencies from their prevalence in human blood samples. This should enhance the use of frequency data to inform antimalarial drug policy choice. MalHaploFreq: A computer programme for estimating malaria haplotype frequencies from blood samples Ian Hastings Thomas Smith doi:10.1186/1475-2875-7-130 Malaria Journal, Vol. 7, No. 1. (2008) 2008-07-15T12:53:03-00:00 2008 Malaria Journal 7 1 http://www.citeulike.org/article/3000344 <i>Malaria Journal, Vol. 7, No. 1. (2008)</i><br /><br />BACKGROUND:Antibodies are the main effectors against malaria blood-stage parasites. Evaluation of functional activities in immune sera from Phase 2a/b vaccine trials may provide invaluable information in the search for immune correlates of protection. However, the presence of anti-malarial-drugs, improper collection/storage conditions or concomitant immune responses against other pathogens can contribute to non-specific anti-parasite activities when the sera/plasma are tested in vitro. Purification of immunoglobulin is a standard approach for reducing such non-specific background activities, but the purification method itself can alter the quality and yield of recovered Ag-specific antibodies. METHODS:To address this concern, various immunoglobulin (Ig) purification methods (protein G Sepharose, protein A/G Sepharose, polyethylene glycol and caprylic acid-ammonium sulphate precipitation) were evaluated for their impact on the quality, quantity and functional activity of purified rabbit and human Igs. The recovered Igs were analysed for yield and purity by SDS-PAGE, for quality by Ag-specific ELISAs (determining changes in titer, avidity and isotype distribution) and for functional activity by in vitro parasite growth inhibition assay (GIA). RESULTS:This comparison demonstrated that overall polyethylene glycol purification of human serum/plasma samples and protein G Sepharose purification of rabbit sera are optimal for recovering functional Ag-specific antibodies. CONCLUSION:Consequently, critical consideration of the purification method is required to avoid selecting non-representative populations of recovered Ig, which could influence interpretations of vaccine efficacy, or affect the search for immune correlates of protection. Evaluation of immunoglobulin purification methods and their impact on quality and yield of antigen-specific antibodies Elke Leitner Ryan Mease Elizabeth Duncan Farhat Khan John Waitumbi Evelina Angov doi:10.1186/1475-2875-7-129 Malaria Journal, Vol. 7, No. 1. (2008) 2008-07-14T23:05:14-00:00 2008 Malaria Journal 7 1 http://www.citeulike.org/article/3003224 <i>Malaria Journal, Vol. 7 (14 July 2008), 128.</i> Performance of self-diagnosis and standby treatment of malaria in international oilfield service employees in the field Anna Roukens Johannes Berg Alex Barbey Leo Visser doi:10.1186/1475-2875-7-128 Malaria Journal, Vol. 7 (14 July 2008), 128. 2008-07-15T13:21:37-00:00 2008 Malaria Journal 1475-2875 7 128 http://www.citeulike.org/article/2995355 <i>Malaria Journal, Vol. 7 (11 July 2008), 127.</i> Amodiaquine-artesunate vs artemether-lumefantrine for uncomplicated malaria in Ghanaian children: a randomized efficacy and safety trial with one year follow-up George Adjei Jorgen Kurtzhals Onike Rodrigues Michael Alifrangis Lotte Hoegberg Emmanuel Kitcher Ebenezer Badoe Roberta Lamptey Bamenla Goka doi:10.1186/1475-2875-7-127 Malaria Journal, Vol. 7 (11 July 2008), 127. 2008-07-12T12:29:23-00:00 2008 Malaria Journal 1475-2875 7 127 http://www.citeulike.org/article/2995354 <i>Malaria Journal, Vol. 7 (11 July 2008), 126.</i> The usefulness of a new rapid diagnostic test, the First Response(R) Malaria Combo (pLDH/HRP2) card test, for malaria diagnosis in the forested belt of central India Praveen Bharti Nipun Silawat Puspendra Singh Mrigendra Singh Manmohan Shukla Gyan Chand Aditya Dash Neeru Singh doi:10.1186/1475-2875-7-126 Malaria Journal, Vol. 7 (11 July 2008), 126. 2008-07-12T12:29:23-00:00 2008 Malaria Journal 1475-2875 7 126 http://www.citeulike.org/article/2976422 <i>Malaria Journal, Vol. 7 (09 July 2008), 125.</i> Reduction of transmission from malaria patients by artemisinin combination therapies: a pooled analysis of six randomized trials Lucy Okell Chris Drakeley Azra Ghani Teun Bousema Colin Sutherland doi:10.1186/1475-2875-7-125 Malaria Journal, Vol. 7 (09 July 2008), 125. 2008-07-09T12:26:04-00:00 2008 Malaria Journal 1475-2875 7 125 http://www.citeulike.org/article/2976421 <i>Malaria Journal, Vol. 7 (08 July 2008), 124.</i> Antimalarial drug use in general populations of tropical Africa Florence Gardella Serge Assi Fabrice Simon Herve Bogreau Teunis Eggelte Fatou Ba Vincent Foumane Marie-Claire Henry Pelagie Kientaga Leonardo Basco Jean-Francois Trape Richard Lalou Maryse Martelloni Marc Desbordes Meili Baragatti Sebastien Briolant Lionel Almeras Bruno Pradines Thierry Fusai Christophe Rogier doi:10.1186/1475-2875-7-124 Malaria Journal, Vol. 7 (08 July 2008), 124. 2008-07-09T12:26:03-00:00 2008 Malaria Journal 1475-2875 7 124 http://www.citeulike.org/article/2976420 <i>Malaria Journal, Vol. 7 (08 July 2008), 123.</i> Impact of intermittent preventive treatment with sulphadoxine-pyrimethamine targeting the transmission season on the incidence of clinical malaria in children in Mali Alassane Dicko Issaka Sagara Mahamadou Sissoko Ousmane Guindo Abdoulbaki Diallo Mamady Kone Ousmane Toure Massambou Sacko Ogobara Doumbo doi:10.1186/1475-2875-7-123 Malaria Journal, Vol. 7 (08 July 2008), 123. 2008-07-09T12:26:03-00:00 2008 Malaria Journal 1475-2875 7 123 http://www.citeulike.org/article/2976419 <i>Malaria Journal, Vol. 7 (08 July 2008), 122.</i> No PfATPase6 S769N mutation found in Plasmodium falciparum isolates from China Guoqing Zhang Yayi Guan Bin Zheng Song Wu Linhua Tang doi:10.1186/1475-2875-7-122 Malaria Journal, Vol. 7 (08 July 2008), 122. 2008-07-09T12:26:03-00:00 2008 Malaria Journal 1475-2875 7 122 http://www.citeulike.org/article/2965233 <i>Malaria Journal, Vol. 7 (04 July 2008), 121.</i> Antibody responses to the merozoite surface protein-1 complex in cerebral malaria patients in India Naomi Lucchi Jon Tongren Vidhan Jain Avinash Nagpal Christian Kauth Ute Woehlbier Hermann Bujard Aditya Dash Neeru Singh Jonathan Stiles Venkatachalam Udhayakumar doi:10.1186/1475-2875-7-121 Malaria Journal, Vol. 7 (04 July 2008), 121. 2008-07-05T00:19:58-00:00 2008 Malaria Journal 1475-2875 7 121 http://www.citeulike.org/article/2965232 <i>Malaria Journal, Vol. 7 (04 July 2008), 120.</i> Population structure of the malaria vector Anopheles moucheti in the equatorial forest region of Africa Christophe Antonio-Nkondjio Cyrille Ndo Pierre Kengne Louis Mukwaya Parfait Awono-Ambene Didier Fontenille Frederic Simard doi:10.1186/1475-2875-7-120 Malaria Journal, Vol. 7 (04 July 2008), 120. 2008-07-05T00:19:58-00:00 2008 Malaria Journal 1475-2875 7 120 http://www.citeulike.org/article/2965231 <i>Malaria Journal, Vol. 7 (03 July 2008), 119.</i> Recent reduction in the water level of Lake Victoria has created more habitats for Anopheles funestus Noboru Minakawa George Sonye Gabriel Dida Kyoko Futami Satoshi Kaneko doi:10.1186/1475-2875-7-119 Malaria Journal, Vol. 7 (03 July 2008), 119. 2008-07-05T00:19:58-00:00 2008 Malaria Journal 1475-2875 7 119 http://www.citeulike.org/article/2965230 <i>Malaria Journal, Vol. 7 (03 July 2008), 118.</i> Evaluation of light microscopy and rapid diagnostic test for the detection of malaria under operational field conditions: a household survey in Ethiopia Tekola Endeshaw Teshome Gebre Jeremiah Ngondi Patricia Graves Estifanos Shargie Yeshewamebrat Ejigsemahu Berhan Ayele Gedeon Yohannes Tesfaye Teferi Ayenew Messele Mulat Zerihun Asrat Genet Aryc Mosher Paul Emerson Frank Richards doi:10.1186/1475-2875-7-118 Malaria Journal, Vol. 7 (03 July 2008), 118. 2008-07-05T00:19:58-00:00 2008 Malaria Journal 1475-2875 7 118 http://www.citeulike.org/article/2958881 <i>Malaria Journal, Vol. 7 (03 July 2008), 117.</i> Congenital Plasmodium falciparum infection in neonates in Muheza District, Tanzania Grace Mwangoka Sharadhuli Kimera Leonard Mboera doi:10.1186/1475-2875-7-117 Malaria Journal, Vol. 7 (03 July 2008), 117. 2008-07-03T12:17:46-00:00 2008 Malaria Journal 1475-2875 7 117 http://www.citeulike.org/article/2949519 <i>Malaria Journal, Vol. 7 (01 July 2008), 116.</i> SURFIN4.1, a schizont-merozoite associated protein in the SURFIN family of Plasmodium falciparum Fingani Mphande Ulf Ribacke Osamu Kaneko Fred Kironde Gerhard Winter Mats Wahlgren doi:10.1186/1475-2875-7-116 Malaria Journal, Vol. 7 (01 July 2008), 116. 2008-07-02T00:11:02-00:00 2008 Malaria Journal 1475-2875 7 116 http://www.citeulike.org/article/2949518 <i>Malaria Journal, Vol. 7 (01 July 2008), 115.</i> Rapid increase of Plasmodium falciparum dhfr/dhps resistant haplotypes, after the adoption of sulphadoxine-pyrimethamine as first line treatment in 2002, in southern Mozambique Sonia Enosse Pascal Magnussen Fatima Abacassamo Xavier Gomez-Olive Anita Ronn Richardo Thompson Michael Alifrangis doi:10.1186/1475-2875-7-115 Malaria Journal, Vol. 7 (01 July 2008), 115. 2008-07-02T00:11:02-00:00 2008 Malaria Journal 1475-2875 7 115 http://www.citeulike.org/article/2936423 <i>Malaria Journal, Vol. 7 (27 June 2008), 114.</i> Median knock-down time as a new method for evaluating insecticide-treated textiles for mosquito control Ole Skovmand Julien Bonnet Olivier Pigeon Vincent Corbel doi:10.1186/1475-2875-7-114 Malaria Journal, Vol. 7 (27 June 2008), 114. 2008-06-27T11:23:56-00:00 2008 Malaria Journal 1475-2875 7 114 http://www.citeulike.org/article/2936422 <i>Malaria Journal, Vol. 7 (26 June 2008), 113.</i> Suppression of Plasmodium falciparum by serum collected from a case of Plasmodium vivax infection Yoshiro Nagao Masako Kimura-Sato Porntip Chavalitshewinkoon-Petmitr Supatra Thongrungkiat Polrat Wilairatana Takafumi Ishida Peerapan Tan-Ariya Brian de Souza Srivicha Krudsood Sornchai Looareesuwan doi:10.1186/1475-2875-7-113 Malaria Journal, Vol. 7 (26 June 2008), 113. 2008-06-27T11:23:55-00:00 2008 Malaria Journal 1475-2875 7 113 http://www.citeulike.org/article/2936421 <i>Malaria Journal, Vol. 7 (26 June 2008), 112.</i> Polymorphism patterns in Duffy-binding protein among Thai Plasmodium vivax isolates Panita Gosi Srisin Khusmith Thareerat Khalambaheti David Lanar Kurt Schaecher Mark Fukuda Scott Miller doi:10.1186/1475-2875-7-112 Malaria Journal, Vol. 7 (26 June 2008), 112. 2008-06-27T11:23:55-00:00 2008 Malaria Journal 1475-2875 7 112 http://www.citeulike.org/article/2923122 <i>Malaria Journal, Vol. 7 (23 June 2008), 111.</i> Spatially-explicit risk profiling of Plasmodium falciparum infections at a small scale: a geostatistical modelling approach Kigbafori Silue Giovanna Raso Ahoua Yapi Penelope Vounatsou Marcel Tanner Eliezer N'goran Juerg Utzinger doi:10.1186/1475-2875-7-111 Malaria Journal, Vol. 7 (23 June 2008), 111. 2008-06-24T10:34:24-00:00 2008 Malaria Journal 1475-2875 7 111 http://www.citeulike.org/article/2908634 <i>Malaria Journal, Vol. 7 (19 June 2008), 110.</i> One-year delayed effect of fog on malaria transmission: a time-series analysis in the rain forest area of Mengla County, south-west China Linwei Tian Yan Bi Suzanne Ho Wenjie Liu Song Liang William Goggins Emily Chan Shuisen Zhou Joseph Sung doi:10.1186/1475-2875-7-110 Malaria Journal, Vol. 7 (19 June 2008), 110. 2008-06-19T20:56:26-00:00 2008 Malaria Journal 1475-2875 7 110 http://www.citeulike.org/article/2892541 <i>Malaria Journal, Vol. 7 (12 June 2008), 109.</i> Full blood count and haemozoin-containing leukocytes in children with malaria: diagnostic value and association with disease severity Thomas Haenscheid Matthias Laengin Bertrand Lell Marc Poetschke Sunny Oyakhirome Peter Kremsner Martin Grobusch doi:10.1186/1475-2875-7-109 Malaria Journal, Vol. 7 (12 June 2008), 109. 2008-06-13T19:11:33-00:00 2008 Malaria Journal 1475-2875 7 109 http://www.citeulike.org/article/2891702 <i>Malaria Journal, Vol. 7, No. 1. (2008)</i><br /><br />BACKGROUND:The level of antibodies against PfEMP1 is routinely quantified by the conventional microtitre enzyme-linked immunosorbent assay (ELISA). However, ELISA only measures one analyte at a time and requires a relatively large plasma volume if the complete antibody profile of the sample is to be obtained. Furthermore, assay-to-assay variation and problem of storage of antigen can influence ELISA results. The bead-based assay described here uses the BioPlex100 (BioRad, Hercules, CA, USA) system which can quantify multiple antibodies simultaneously in a small plasma volume. METHODS:A total of twenty nine PfEMP1 domains were PCR amplified from 3D7 genomic DNA, expressed in the Baculovirus system and purified by metal-affinity chromatography. The antibody reactivity level to the recombinant PfEMP1 proteins in human hyper-immune plasma was measured by ELISA. In parallel, these recombinant PfEMP1 proteins were covalently coupled onto beads each having its own unique detection signal and the human hyper-immune plasma reactivity was detected for each individual protein using a BioPlex100 system. Protein-coupled beads were analysed at two time points seven months apart, before and after lyophilization and the results compared to determine the effect of storage and lyophilization respectively on the beads. Multiplexed protein-coupled beads from twenty eight unique bead populations were evaluated on the BioPlex100 system against pooled human hyper-immune plasma before and after lyophilization.RESULTS:The bead-based assay was sensitive, accurate and reproducible. Four recombinant PfEMP1 proteins C17, D5, D9 and D12, selected on the basis that they showed a spread of MFI values from low to high when analysed by the bead-based assay were analysed by ELISA and the results from both analyses were highly correlated. The Spearman's rank correlation coefficients (Rho) were [greater than or equal to] 0.86, (P < 0.0001) for all comparisons. Bead-based assays gave similar results regardless of whether they were performed on individual beads or on multiplex beads; lyophilization had no impact on the assay performance. Spearman's rank correlation coefficients (Rho) were [greater than or equal to] 0.97, (P < 0.0001) for all comparisons. Importantly, the reactivity of protein-coupled non-lyophilized beads decreased with long term storage at 4 OC in the dark. CONCLUSION:Using this lyophilized multiplex assay, antibody reactivity levels to twenty eight different recombinant PfEMP1 proteins were simultaneously measured using a single microliter of plasma. Thus, the assay reported here provides a useful tool for rapid and efficient quantification of antibody reactivity against PfEMP1 variants in human plasma. A semi-automated multiplex high-throughput assay for measuring IgG antibodies against Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) domains in small volumes of plasma Gerald Cham Jonathan Kurtis John Lusingu Thor Theander Anja Jensen Louise Turner doi:10.1186/1475-2875-7-108 Malaria Journal, Vol. 7, No. 1. (2008) 2008-06-13T15:54:22-00:00 2008 Malaria Journal 7 1