Thu, 21 Aug 2008 15:10:39 BST CiteULike: AlfonsoVicenteSuarez's library [225 articles] http://www.citeulike.org/user/AlfonsoVicenteSuarez/article/1447284 CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/AlfonsoVicenteSuarez/article/1447284 <i>Immunity, Vol. 11, No. 6. (1 December 1999), pp. 665-675.</i><br /><br />Nucleosomes are important for gene regulation, but comprehensive studies of nucleosome positioning, remodeling, and transcription factor binding at inducible mammalian promoters have not been reported. We have analyzed the IL-12 p40 promoter, which is induced in macrophages by bacterial products. High-resolution micrococcal nuclease analyses revealed that a positioned nucleosome, nucleosome 1, spans the promoter, with three positioned nucleosomes further upstream. Upon activation, nucleosome 1 was rapidly and selectively remodeled in a protein synthesis-dependent manner. In primary macrophages, IFN[gamma] synergistically enhanced p40 expression, but little effect on remodeling or promoter occupancy was observed. These results suggest that remodeling complexes are selectively targeted to a single, promoter-encompassing nucleosome and that IFN[gamma] influences an event that is independent or downstream of remodeling. Rapid and Selective Remodeling of a Positioned Nucleosome during the Induction of IL-12 p40 Transcription Amy Weinmann Scott Plevy Stephen Smale doi:10.1016/S1074-7613(00)80141-7 Immunity, Vol. 11, No. 6. (1 December 1999), pp. 665-675. 2007-07-10T20:20:38-00:00 1999 Immunity 11 6 665 675 il-12-general