Sat, 26 Jul 2008 05:57:25 BST CiteULike: brembs's Kohn http://www.citeulike.org/user/brembs/author/Kohn CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/brembs/article/1023152 <i>Cell, Vol. 127, No. 7. (29 December 2006), pp. 1453-1467.</i><br /><br />SummaryMolecular analyses of Aplysia, a well-established model organism for cellular and systems neural science, have been seriously handicapped by a lack of adequate genomic information. By sequencing cDNA libraries from the central nervous system (CNS), we have identified over 175,000 expressed sequence tags (ESTs), of which 19,814 are unique neuronal gene products and represent 50%-70% of the total Aplysia neuronal transcriptome. We have characterized the transcriptome at three levels: (1) the central nervous system, (2) the elementary components of a simple behavior: the gill-withdrawal reflex--by analyzing sensory, motor, and serotonergic modulatory neurons, and (3) processes of individual neurons. In addition to increasing the amount of available gene sequences of Aplysia by two orders of magnitude, this collection represents the largest database available for any member of the Lophotrochozoa and therefore provides additional insights into evolutionary strategies used by this highly successful diversified lineage, one of the three proposed superclades of bilateral animals. Neuronal Transcriptome of Aplysia: Neuronal Compartments and Circuitry Leonid Moroz John Edwards Sathyanarayanan Puthanveettil Andrea Kohn Thomas Ha Andreas Heyland Bjarne Knudsen Anuj Sahni Fahong Yu Li Liu doi:10.1016/j.cell.2006.09.052 Cell, Vol. 127, No. 7. (29 December 2006), pp. 1453-1467. 2007-01-03T10:18:42-00:00 2006 Cell 127 7 1453 1467 aplysia classical conditioning instrumental invertebrate neurobiology neuroscience operant pavlovian