Sat, 26 Jul 2008 06:13:58 BST CiteULike: dchen's Fujiwara http://www.citeulike.org/user/dchen/author/Fujiwara CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/dchen/article/2759188 <i>Physical Review Letters, Vol. 100, No. 16. (2008)</i><br /><br />Fluorescence spectroscopy of single proteins at liquid-helium temperatures reveals a relation between structural dynamics and biological functions of the proteins. The technical difficulties in detecting visible fluorescence are chromatic aberration and optical background. They were overcome by a new optical design using reflective optics and employing two-photon excitation. The fluorescence spectrum of single green-fluorescent proteins taken at a temperature of 1.5 K makes a distinction between different metastable conformations that last for tens of seconds. Visible Fluorescence Spectroscopy of Single Proteins at Liquid-Helium Temperature Satoru Fujiyoshi Masanori Fujiwara Michio Matsushita doi:10.1103/PhysRevLett.100.168101 Physical Review Letters, Vol. 100, No. 16. (2008) 2008-05-05T20:53:11-00:00 2008 Physical Review Letters 100 16 APS 2008 biology microscope protein structure technique