Sun, 27 Jul 2008 08:03:22 BST CiteULike: dna's Gothard http://www.citeulike.org/user/dna/author/Gothard CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/dna/article/2762826 <i>J. Am. Chem. Soc., Vol. 129, No. 23. (13 June 2007), pp. 7272-7273.</i><br /><br />Abstract: This paper introduces the unnatural amino acid Abc2K as a nanometer-length building block for the creation of water-soluble molecular rods of exceptional size. Abc2K is a water-soluble variant of the unnatural amino acid 4'-amino-[1,1'-biphenyl]-4-carboxylic acid (Abc) with lysinelike propyloxyammonium side chains at the 2- and 5-positions. The protected building block Fmoc-Abc2K(Boc)-OH (1) can be used in standard Fmoc-based solid-phase peptide synthesis to create water-soluble rodlike peptides in nanometer unit lengths up to at least ten nanometers. Oligomers up to and including the decamer were easily prepared on a Rink amide resin. These peptides are easy to purify and characterize by standard reverse-phase HPLC, 1H NMR, and ESI-MS techniques. The Abc2K amino acid can be combined with various standard amino acids to provide well behaved hybrid and biologically relevant peptides. Building block 1 is efficiently prepared on a multigram scale from commercially available starting materials by way of the Suzuki cross-coupling reaction. Molecular modeling studies of Abc2K oligomers show only minor effects from torsional and bending motions and support a model in which the oligomers are relatively straight and rigid. Fluorescence resonance energy transfer (FRET) studies are consistent with a model in which the Abc2K oligomers behave as rigid rods with a length of 1.0 nm per monomer unit. Nanometer-Sized Amino Acids for the Synthesis of Nanometer-Scale Water-Soluble Molecular Rods of Precise Length CM Gothard NA Rao JS Nowick doi:10.1021/ja072648i J. Am. Chem. Soc., Vol. 129, No. 23. (13 June 2007), pp. 7272-7273. 2008-05-06T20:20:45-00:00 2007 J. Am. Chem. Soc. 129 23 7272 7273 origami origami-alternatives