Thu, 21 Aug 2008 07:06:11 BST CiteULike: emptyhb's Longabaugh http://www.citeulike.org/user/emptyhb/author/Longabaugh CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/emptyhb/article/2175731 <i>Cell, Vol. 131, No. 7. (28 December 2007), pp. 1354-1365.</i><br /><br />Summary The environment significantly influences the dynamic expression and assembly of all components encoded in the genome of an organism into functional biological networks. We have constructed a model for this process in Halobacterium salinarum NRC-1 through the data-driven discovery of regulatory and functional interrelationships among ~80% of its genes and key abiotic factors in its hypersaline environment. Using relative changes in 72 transcription factors and 9 environmental factors (EFs) this model accurately predicts dynamic transcriptional responses of all these genes in 147 newly collected experiments representing completely novel genetic backgrounds and environments--suggesting a remarkable degree of network completeness. Using this model we have constructed and tested hypotheses critical to this organism's interaction with its changing hypersaline environment. This study supports the claim that the high degree of connectivity within biological and EF networks will enable the construction of similar models for any organism from relatively modest numbers of experiments. A Predictive Model for Transcriptional Control of Physiology in a Free Living Cell Richard Bonneau Marc Facciotti David Reiss Amy Schmid Min Pan Amardeep Kaur Vesteinn Thorsson Paul Shannon Michael Johnson Christopher Bare William Longabaugh Madhavi Vuthoori Kenia Whitehead Aviv Madar Lena Suzuki Tetsuya Mori Dong-Eun Chang Jocelyne Diruggiero Carl Johnson Leroy Hood Nitin Baliga doi:10.1016/j.cell.2007.10.053 Cell, Vol. 131, No. 7. (28 December 2007), pp. 1354-1365. 2007-12-27T20:18:55-00:00 2007 Cell 131 7 1354 1365 modeling_gene_network