Thu, 21 Aug 2008 09:48:30 BST CiteULike: kevinchannon's microfluidics http://www.citeulike.org/user/kevinchannon/tag/microfluidics CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/kevinchannon/article/2770559 <i>J. Am. Chem. Soc., Vol. 129, No. 42. (24 October 2007), pp. 12608-12609.</i><br /><br />Abstract: We report a straightforward method for the preparation of lipid vesicles inspired by forming soap bubbles from a soap film. Vesicles are mechanically blown out of a preformed biofunctional planar lipid membrane by applying a short pulsed liquid jet flow ejected from a fine jet nozzle brought near the membrane. This method allows us to directly encapsulate any molecules or substances of interest into the uniformly sized vesicles in a short time. Formation of Giant Lipid Vesiclelike Compartments from a Planar Lipid Membrane by a Pulsed Jet Flow K Funakoshi H Suzuki S Takeuchi doi:10.1021/ja074029f J. Am. Chem. Soc., Vol. 129, No. 42. (24 October 2007), pp. 12608-12609. 2008-05-08T09:59:39-00:00 2007 J. Am. Chem. Soc. 129 42 12608 12609 amphiphiles biophysics lipid microfluidics vesicle http://www.citeulike.org/user/kevinchannon/article/2615850 <i>Chemical Communications (2007), pp. 1773-1788.</i><br /><br />By compartmentalizing reactions in aqueous microdroplets of water-in-oil emulsions, reaction volumes can be reduced by factors of up to 109 compared to conventional microtitre-plate based systems. This allows massively parallel processing of as many as 10^10 reactions in a total volume of only 1 ml of emulsion. This review describes the use of emulsions for directed evolution of proteins and RNAs, and for performing polymerase chain reactions (PCRs). To illustrate these applications we describe certain specific experiments, each of which exemplifies a different facet of the technique, in some detail. These examples include directed evolution of Diels–Alderase and RNA ligase ribozymes and several classes of protein enzymes, including DNA polymerases, phosphotriesterases, beta-galactosidases and thiolactonases. We also describe the application of emulsion PCR to screen for rare mutations and for new ultra-high throughput sequencing technologies. Finally, we discuss the recent development of microfluidic tools for making and manipulating microdroplets and their likely impact on the future development of the field. Miniaturizing chemistry and biology in microdroplets Bernard Kelly Jean-Christophe Baret Valerie Taly Andrew Griffiths Chemical Communications (2007), pp. 1773-1788. 2008-03-31T10:54:32-00:00 2007 Chemical Communications 1773 1788 microfluidics molecular-biology synthetic-biology http://www.citeulike.org/user/kevinchannon/article/805246 <i>Trends in Biotechnology, Vol. 24, No. 9. (September 2006), pp. 395-402.</i><br /><br />Biochemical and genetic assays can be both miniaturized and parallelized by compartmentalization in living cells. In vitro compartmentalization (IVC) offers an alternative strategy based on partitioning reactions in water droplets dispersed to form microscopic compartments in water-in-oil emulsions. The cell-like volumes of these compartments (as low as one femtolitre), the ability to freely determine and regulate their content and the large number of compartments (>1010 per millilitre emulsion) have provided the basis for a range of new, ultra-high-throughput, cell-free technologies. This review describes the scope and potential of IVC in areas such as in vitro evolution of proteins and RNAs, cell-free cloning and sequencing, genetics, genomics, and proteomics. Miniaturising the laboratory in emulsion droplets Andrew Griffiths Dan Tawfik doi:10.1016/j.tibtech.2006.06.009 Trends in Biotechnology, Vol. 24, No. 9. (September 2006), pp. 395-402. 2006-08-18T11:22:00-00:00 2006 Trends in Biotechnology 24 9 395 402 microfluidics molecular-biology self-assembly synthetic-biology http://www.citeulike.org/user/kevinchannon/article/2565693 <i>molecular Systems biology, Vol. 3 (10 July 2007)</i><br /><br />Biological systems display a functional diversity, density and efficiency that make them a paradigm for synthetic systems. In natural systems, the cell is the elemental unit and efforts to emulate cells, their components, and organization have relied primarily on the use of bioorganic materials. Impressive advances have been made towards assembling simple genetic systems within cellular scale containers. These biological system assembly efforts are particularly instructive, as we gain command over the directed synthesis and assembly of synthetic nanoscale structures. Advances in nanoscale fabrication, assembly, and characterization are providing the tools and materials for characterizing and emulating the smallest scale features of biology. Further, they are revealing unique physical properties that emerge at the nanoscale. Realizing these properties in useful ways will require attention to the assembly of these nanoscale components. Attention to systems biology principles can lead to the practical development of nanoscale technologies with possible realization of synthetic systems with cell-like complexity. In turn, useful tools for interpreting biological complexity and for interfacing to biological processes will result. Nano-enabled synthetic biology Mitchel Doktycz Michael Simpson molecular Systems biology, Vol. 3 (10 July 2007) 2008-03-20T09:58:18-00:00 2007 molecular Systems biology 3 encapsulation lipid microfluidics self-assembly synthetic-biology http://www.citeulike.org/user/kevinchannon/article/2340302 <i>J Am Chem Soc, Vol. 126, No. 9. (Mar 2004), pp. 2674-2675.</i> Controlled vesicle self-assembly in microfluidic channels with hydrodynamic focusing Andreas Jahn Wyatt Vreeland Michael Gaitan Laurie Locascio J Am Chem Soc, Vol. 126, No. 9. (Mar 2004), pp. 2674-2675. 2008-02-06T10:37:27-00:00 2004 J Am Chem Soc 126 9 2674 2675 microfluidics self-assembly vesicle