Thu, 21 Aug 2008 14:43:37 BST CiteULike: maralena's Bang http://www.citeulike.org/user/maralena/author/Bang CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/maralena/article/1270222 <i>Nat Med (29 April 2007)</i><br /><br />Growing evidence indicates that microRNAs (miRNAs or miRs) are involved in basic cell functions and oncogenesis. Here we report that miR-133 has a critical role in determining cardiomyocyte hypertrophy. We observed decreased expression of both miR-133 and miR-1, which belong to the same transcriptional unit, in mouse and human models of cardiac hypertrophy. In vitro overexpression of miR-133 or miR-1 inhibited cardiac hypertrophy. In contrast, suppression of miR-133 by 'decoy' sequences induced hypertrophy, which was more pronounced than that after stimulation with conventional inducers of hypertrophy. In vivo inhibition of miR-133 by a single infusion of an antagomir caused marked and sustained cardiac hypertrophy. We identified specific targets of miR-133: RhoA, a GDP-GTP exchange protein regulating cardiac hypertrophy; Cdc42, a signal transduction kinase implicated in hypertrophy; and Nelf-A/WHSC2, a nuclear factor involved in cardiogenesis. Our data show that miR-133, and possibly miR-1, are key regulators of cardiac hypertrophy, suggesting their therapeutic application in heart disease. MicroRNA-133 controls cardiac hypertrophy. Alessandra Carè Daniele Catalucci Federica Felicetti Désirée Bonci Antonio Addario Paolo Gallo Marie-Louise Bang Patrizia Segnalini Yusu Gu Nancy D Dalton Leonardo Elia Michael V G Latronico Morten Høydal Camillo Autore Matteo A Russo Gerald W Dorn Oyvind Ellingsen Pilar Ruiz-Lozano Kirk L Peterson Carlo M Croce Cesare Peschle Gianluigi Condorelli doi:10.1038/nm1582 Nat Med (29 April 2007) 2007-05-01T14:00:18-00:00 2007 Nat Med 1078-8956 cardiogenesis microrna mir-133