Thu, 21 Aug 2008 14:42:41 BST CiteULike: matthewhflamm's Burgreen http://www.citeulike.org/user/matthewhflamm/author/Burgreen CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/matthewhflamm/article/2683112 <i>Annals of Biomedical Engineering, Vol. 27, No. 4. (1 July 1999), pp. 449-458.</i><br /><br />We have previously described the development of a two-dimensional computational model of platelet deposition onto biomaterials from flowing blood (Sorensen et al., Ann. Biomed. Eng. 27:436–448, 1999). The model requires estimation of four parameters to fit it to experimental data: shear-dependent platelet diffusivity and three platelet-deposition-related reaction rate constants. These parameters are estimated for platelet deposition onto a collagen substrate for simple parallel-plate flow of whole blood in both the presence and absence of thrombin. One set of experimental results is used as a benchmark for model-fitting purposes. The “trained” model is then validated by applying it to additional test cases from the literature for parallel-plate Poiseuille flow over collagen at both higher and lower wall shear rates, and in the presence of various anticoagulants. The predicted values agree very well with the experimental results for the training cases, and good reproduction of deposition trends and magnitudes is obtained for the heparin, but not the citrate, validation cases. The model is formulated to be easily extended to synthetic biomaterials, as well as to more complex flows. © 1999 Biomedical Engineering Society. Computational Simulation of Platelet Deposition and Activation: II. Results for Poiseuille Flow over Collagen Erik Sorensen Greg Burgreen William Wagner James Antaki doi:10.1114/1.201 Annals of Biomedical Engineering, Vol. 27, No. 4. (1 July 1999), pp. 449-458. 2008-04-17T18:17:08-00:00 1999 Annals of Biomedical Engineering 27 4 449 458 computational_simulation deposition platelet http://www.citeulike.org/user/matthewhflamm/article/2683110 <i>Annals of Biomedical Engineering, Vol. 27, No. 4. (1 July 1999), pp. 436-448.</i><br /><br />To better understand the mechanisms leading to the formation and growth of mural thrombi on biomaterials, we have developed a two-dimensional computational model of platelet deposition and activation in flowing blood. The basic formulation is derived from prior work by others, with additional levels of complexity added where appropriate. It is comprised of a series of convection-diffusion-reaction equations which simulate platelet-surface and platelet-platelet adhesion, platelet activation by a weighted linear combination of agonist concentrations, agonist release and synthesis by activated platelets, platelet-phospholipid-dependent thrombin generation, and thrombin inhibition by heparin. The model requires estimation of four parameters to fit it to experimental data: shear-dependent platelet diffusivity and resting and activated platelet-surface and platelet-platelet reaction rate constants. The model is formulated to simulate a wide range of biomaterials and complex flows. In this article we present the basic model and its properties; in Part II (Sorensen et al., Ann. Biomed. Eng. 27:449–458, 1999) we apply the model to experimental results for platelet deposition onto collagen. © 1999 Biomedical Engineering Society. Computational Simulation of Platelet Deposition and Activation: I. Model Development and Properties Erik Sorensen Greg Burgreen William Wagner James Antaki doi:10.1114/1.200 Annals of Biomedical Engineering, Vol. 27, No. 4. (1 July 1999), pp. 436-448. 2008-04-17T18:16:07-00:00 1999 Annals of Biomedical Engineering 27 4 436 448 computational_simulation deposition platelet