Sat, 26 Jul 2008 07:29:26 BST CiteULike: matthewhflamm's tissue_factor http://www.citeulike.org/user/matthewhflamm/tag/tissue_factor CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/matthewhflamm/article/2682989 <i>Blood, Vol. 111, No. 7. (1 April 2008), pp. 3507-3513.</i><br /><br />Protein microarrays presenting spots of collagen and lipidated tissue factor (TF) allowed a determination of the critical surface concentration of TF required to trigger coagulation under flow. Whole blood supplemented with corn trypsin inhibitor (to inhibit factor XIIa) was perfused over microarrays for 5 minutes. Immunofluorescence staining of platelet glycoprotein GPIbalpha and fibrin(ogen) revealed a critical TF concentration (EC50) of 3.6, 8.4, and 10.2 molecules-TF/microm2 at wall shear rates of 100, 500, and 1000 s1, respectively. For collagen arrays where only the center lane of spots (in the direction of flow) contained TF, a downstream distance of 14 mm was required for the thrombus to widen enough to reach across a 300-micrometer gap to the adjacent TF-free lanes of collagen spots, in agreement with numerical simulation. To investigate the effect of low levels of circulating TF, whole blood (+/- 100 fM added TF) was tested under static and flow conditions. After 5 minutes, the addition of 100 fM TF to whole blood had negligible effect under static conditions, but caused a 2.5-fold increase in fibrin formation under flow. This report defines the threshold concentrations of surface TF required to trigger coagulation under flow. 10.1182/blood-2007-08-106229 Determination of surface tissue factor thresholds that trigger coagulation at venous and arterial shear rates: amplification of 100 fM circulating tissue factor requires flow Uzoma Okorie William Denney Manash Chatterjee Keith Neeves Scott Diamond doi:10.1182/blood-2007-08-106229 Blood, Vol. 111, No. 7. (1 April 2008), pp. 3507-3513. 2008-04-17T17:16:41-00:00 2008 Blood 111 7 3507 3513 flow tissue_factor