Sat, 05 Jul 2008 22:58:01 BST CiteULike: neils's Waldo http://www.citeulike.org/user/neils/author/Waldo CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/neils/article/2319613 <i>Nat Methods, Vol. 5, No. 2. (February 2008), pp. 135-146.</i><br /><br />In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analysis of the expression and purification of over 10,000 different proteins. This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators. Protein production and purification. S Gräslund P Nordlund J Weigelt J Bray O Gileadi S Knapp U Oppermann C Arrowsmith R Hui J Ming S Dhe-Paganon HW Park A Savchenko A Yee A Edwards R Vincentelli C Cambillau R Kim SH Kim Z Rao Y Shi TC Terwilliger CY Kim LW Hung GS Waldo Y Peleg S Albeck T Unger O Dym J Prilusky JL Sussman RC Stevens SA Lesley IA Wilson A Joachimiak F Collart I Dementieva MI Donnelly WH Eschenfeldt Y Kim L Stols R Wu M Zhou SK Burley JS Emtage JM Sauder D Thompson K Bain J Luz T Gheyi F Zhang S Atwell SC Almo JB Bonanno A Fiser S Swaminathan FW Studier MR Chance A Sali TB Acton R Xiao L Zhao LC Ma JF Hunt L Tong K Cunningham M Inouye S Anderson H Janjua R Shastry CK Ho D Wang H Wang M Jiang GT Montelione DI Stuart RJ Owens S Daenke A Schütz U Heinemann S Yokoyama K Büssow KC Gunsalus doi:10.1038/nmeth.f.202 Nat Methods, Vol. 5, No. 2. (February 2008), pp. 135-146. 2008-02-01T14:36:35-00:00 2008 Nat Methods 1548-7105 5 2 135 146 expression genomics proteins purification sg structure