Thu, 21 Aug 2008 14:19:23 BST CiteULike: tardigrade's Welker http://www.citeulike.org/user/tardigrade/author/Welker CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/tardigrade/article/1343825 <i>RNA (25 May 2007)</i><br /><br />We describe the first microarray analysis of a whole animal containing a mutation in the Dicer gene. We used adult Caenorhabditis elegans and, to distinguish among different roles of Dicer, we also performed microarray analyses of animals with mutations in rde-4 and rde-1, which are involved in silencing by siRNA, but not miRNA. Surprisingly, we find that the X chromosome is greatly enriched for genes regulated by Dicer. Comparison of all three microarray data sets indicates the majority of Dicer-regulated genes are not dependent on RDE-4 or RDE-1, including the X-linked genes. However, all three data sets are enriched in genes important for innate immunity and, specifically, show increased expression of innate immunity genes. Genes misregulated in C. elegans deficient in Dicer, RDE-4, or RDE-1 are enriched for innate immunity genes. Noah C Welker Jeffrey W Habig Brenda L Bass doi:10.1261/rna.542107 RNA (25 May 2007) 2007-05-30T18:02:54-00:00 2007 RNA 1355-8382 nematode rnai sirna