Sun, 27 Jul 2008 09:08:33 BST CiteULike: timyu's genomics http://www.citeulike.org/user/timyu/tag/genomics CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/timyu/article/411161 <i>Nat Rev Genet, Vol. 5, No. 1. (January 2004), pp. 11-22.</i> Systematic genome-wide screens of gene function. AE Carpenter DM Sabatini doi:10.1038/nrg1248 Nat Rev Genet, Vol. 5, No. 1. (January 2004), pp. 11-22. 2005-11-29T09:41:41-00:00 2004 Nat Rev Genet 1471-0056 5 1 11 22 genomics rnai http://www.citeulike.org/user/timyu/article/232589 <i>Cell, Vol. 121, No. 6. (17 June 2005), pp. 837-848.</i><br /><br />Tumorigenesis is a multistep process characterized by a myriad of genetic and epigenetic alterations. Identifying the causal perturbations that confer malignant transformation is a central goal in cancer biology. Here we report an RNAi-based genetic screen for genes that suppress transformation of human mammary epithelial cells. We identified genes previously implicated in proliferative control and epithelial cell function including two established tumor suppressors, TGFBR2 and PTEN. In addition, we uncovered a previously unrecognized tumor suppressor role for REST/NRSF, a transcriptional repressor of neuronal gene expression. Array-CGH analysis identified REST as a frequent target of deletion in colorectal cancer. Furthermore, we detect a frameshift mutation of the REST gene in colorectal cancer cells that encodes a dominantly acting truncation capable of transforming epithelial cells. Cells lacking REST exhibit increased PI(3)K signaling and are dependent upon this pathway for their transformed phenotype. These results implicate REST as a human tumor suppressor and provide a novel approach to identifying candidate genes that suppress the development of human cancer. A Genetic Screen for Candidate Tumor Suppressors Identifies REST. TF Westbrook ES Martin MR Schlabach Y Leng AC Liang B Feng JJ Zhao TM Roberts G Mandel GJ Hannon RA Depinho L Chin SJ Elledge doi:10.1016/j.cell.2005.03.033 Cell, Vol. 121, No. 6. (17 June 2005), pp. 837-848. 2005-06-20T16:47:28-00:00 2005 Cell 0092-8674 121 6 837 848 cancer genomics rnai http://www.citeulike.org/user/timyu/article/368075 <i>Nature Genetics, Vol. 37, No. 11. (02 October 2005), pp. 1281-1288.</i> Second-generation shRNA libraries covering the mouse and human genomes Jose Silva Mamie Li Ken Chang Wei Ge Michael Golding Richard Rickles Despina Siolas Guang Hu Patrick Paddison Michael Schlabach Nihar Sheth Jeff Bradshaw Julia Burchard Amit Kulkarni Guy Cavet Ravi Sachidanandam Richard Mccombie Michele Cleary Stephen Elledge Gregory Hannon doi:10.1038/ng1650 Nature Genetics, Vol. 37, No. 11. (02 October 2005), pp. 1281-1288. 2005-10-27T18:58:30-00:00 2005 Nature Genetics 1061-4036 37 11 1281 1288 Nature Publishing Group genomics rnai http://www.citeulike.org/user/timyu/article/470179 <i>Science, Vol. 310, No. 5747. (21 October 2005), pp. 439-441.</i> An open letter to cancer researchers. SJ Elledge GJ Hannon doi:10.1126/science.310.5747.439b Science, Vol. 310, No. 5747. (21 October 2005), pp. 439-441. 2006-01-19T02:39:21-00:00 2005 Science 1095-9203 310 5747 439 441 cancer genomics rnai http://www.citeulike.org/user/timyu/article/409443 <i>Annu Rev Neurosci, Vol. 28 (2005), pp. 89-108.</i><br /><br />Over the past two decades, molecular genetic studies have enabled a common conceptual framework for the development and basic function of the nervous system. These studies, and the pioneering efforts of mouse geneticists and neuroscientists to identify and clone genes for spontaneous mouse mutants, have provided a paradigm for understanding complex processes of the vertebrate brain. Gene cloning for human brain malformations and degenerative disorders identified other important central nervous system (CNS) genes. However, because many debilitating human disorders are genetically complex, phenotypic screens are difficult to design. This difficulty has led to large-scale, genomic approaches to discover genes that are uniquely expressed in brain circuits and regions that control complex behaviors. In this review, we summarize current phenotype- and genotype-driven approaches to discover novel CNS-expressed genes, as well as current approaches to carry out large-scale, gene-expression screens in the CNS. Large-scale genomic approaches to brain development and circuitry. ME Hatten N Heintz doi:10.1146/annurev.neuro.26.041002.131436 Annu Rev Neurosci, Vol. 28 (2005), pp. 89-108. 2005-11-27T02:08:41-00:00 2005 Annu Rev Neurosci 0147-006X 28 89 108 development genomics techniques