Thu, 21 Aug 2008 14:17:47 BST CiteULike: tny's Tawfik http://www.citeulike.org/user/tny/author/Tawfik CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/tny/article/854207 <i>Current Opinion in Chemical Biology, Vol. 10, No. 5. (October 2006), pp. 498-508.</i><br /><br />The past few years have seen significant advances in research related to the `latent skills' of enzymes -- namely, their capacity to promiscuously catalyze reactions other than the ones they evolved for. These advances regard (i) the mechanism of catalytic promiscuity -- how enzymes, that generally exert exquisite specificity, promiscuously catalyze other, and sometimes barely related, reactions; (ii) the evolvability of promiscuous functions -- namely, how latent activities evolve further, and in particular, how promiscuous activities can firstly evolve without severely compromising the original activity. These findings have interesting implications on our understanding of how new enzymes evolve. They support the key role of catalytic promiscuity in the natural history of enzymes, and suggest that today's enzymes diverged from ancestral proteins catalyzing a whole range of activities at low levels, to create families and superfamilies of potent and highly specialized enzymes. Enzyme promiscuity: evolutionary and mechanistic aspects Olga Khersonsky Cintia Roodveldt Dan Tawfik doi:10.1016/j.cbpa.2006.08.011 Current Opinion in Chemical Biology, Vol. 10, No. 5. (October 2006), pp. 498-508. 2006-09-22T09:15:26-00:00 2006 Current Opinion in Chemical Biology 10 5 498 508 catalysis enzyme evolution http://www.citeulike.org/user/tny/article/73419 <i>Nature Genetics, Vol. 37, No. 1. (28 November 2004), 73.</i> The 'evolvability' of promiscuous protein functions Amir Aharoni Leonid Gaidukov Olga Khersonsky Stephen Gould Cintia Roodveldt Dan Tawfik doi:10.1038/ng1482 Nature Genetics, Vol. 37, No. 1. (28 November 2004), 73. 2005-01-07T13:59:39-00:00 2004 Nature Genetics 1061-4036 37 1 73 Nature Publishing Group engeneering enzyme