Tag dtt [21 articles]

Abstract

The near ubiquity of mobile phones in the world and in people's lives, have created new opportunities for reinterpretation of what these devices are and what they do. One area that has recently emerged is the use of mobile phones in religious practices. Researchers and designers have yet to develop a concrete framework or strategies for designing in this space. ...

Abstract

This paper argues that interface design can be made more effective by borrowing techniques from graphic design. User interface designers often explore interface ideas through coded prototypes, which do not facilitate quick turnaround and require a complete interface definition. This method of prototyping is too detailed and laborious to appropriately facilitate early design decisions, such as brainstorming about the task the interface will support. ...

Abstract

They believe that planes come from paradise---their ancestors sent them. But the white man, a crafty pirate, manages to get his hands on them by attracting them into a big trap of an airport. You build your plane too, and wait with faith. Sooner of later, your ancestors will discover the white man's trap and will guide the planes on your landing strip. Then you will be rich and happy. ---Narration from the film Mondo Cane , 1962 ...

Abstract

Rapid prototyping helps designers explore issues such as form factors and the relationship between the technology and the social environment in which it will be used. This case study provides examples of good design practice for iterative design of tangible user interfaces. Furthermore, the curriculum-focused design approach demonstrates how it's sometimes necessary to design both the technology and the social context of use.This article is part of a special issue on rapid prototyping. ...

Abstract

We present a study comparing usability testing of a single interface versus three functionally equivalent but stylistically distinct designs. We found that when presented with a single design, users give significantly higher ratings and were more reluctant to criticize than when presented with the same design in a group of three. Our results imply that by presenting users with alternative design solutions, subjective ratings are less prone to inflation and give rise to more and stronger criticisms when appropriate. Contrary to ...

Abstract

Researchers at University of California, Berkeley and Carnegie Mellon University have designed, implemented, and evaluated SILK (Sketching Interfaces Like Krazy), an informal sketching tool that combines many of the benefits of paper-based sketching with the merits of current electronic tools. With SILK, designers can quickly sketch an interface using an electronic pad and stylus, and SILK recognizes widgets and other interface elements as the designer draws them. Unlike paper-based sketching, however, designers can exercise these elements in their sketchy state. For ...

Abstract

Designing information architecture for complex websites requires understanding user information needs and mental models in that domain. Personas, or user archetypes, created for such domains should also reflect types of information needs, and usage of information set. We have created a statistical technique to identify important underlying groupings of information needs. In a preliminary study, we show how designers can use this information in conjunction with data from interviews and observations to generate and refine personas. ...

Abstract

In User-Centered Design, there is always the need to precisely define the user attributes, so that the product can be designed based on the patterns of these attributes. The methods of user definitions include quantitative segmentation analysis, as well as qualitative research on the patterns of user behaviors. Because these attributes are often complicated and abstract, researchers may have difficulty communicating these patterns to other team members. ...

Abstract

There are few independent studies reporting on the relation between interaction designers existing practices contra their efficient use of personas. Practitioners have reported that the technique have a potential for software mass-market products, and as a user-information-needs-model. Based on three different software development project characteristics this paper presents an independent study on the usefulness of personas. In the first project, it is demonstrated that personas played a surprisingly little role compared to ...

Abstract

The newly introduced sulfhydryl reductant tris(2-carboxyethyl)phosphine (TCEP) is a potentially attractive alternative to commonly used dithiothreitol (DTT). We compare properties of DTT and TCEP important in protein biochemistry, using the motor enzyme myosin as an example protein. The reductants equally preserve myosin's enzymatic activity, which is sensitive to sulfhydryl oxidation. When labeling with extrinsic probes, DTT inhibits maleimide attachment to myosin and must be removed before labeling. In contrast, maleimide attachment to myosin was achieved in the presence of TCEP, although ...

Abstract

Note: OCR errors may be found in this Reference List extracted from the full text article. ACM has opted to expose the complete List rather than only correct and linked references. ...

Abstract

Evidence is presented for a role of disulfide bridging in forming the ligand binding site of the beta 2-adrenergic receptor (beta AR). The presence of disulfide bonds at the ligand binding site is indicated by "competitive" inhibition by dithiothreitol (DTT) in radioligand binding assays, by specific protection by beta-adrenergic ligands of these effects, and by the requirement of disulfide reduction for limit proteolysis of affinity ligand labeled receptor. The kinetics of binding inhibition by DTT suggest at least two pairs of ...

Abstract

The mobility of purified ? opioid binding protein in SDS-polyacrylamide gek electrophoresis is sensitive to the presence of reducing agents. In the presence of increasing concentrations of DTT the apparent molecular weight increases in a stepwise fashion from 53 kDa to 65 kDa. This reduction in mobility is attributed to the successive breakage of disulfide bridges, resulting in an increasingly asymmetric molecule. Treatment of cell membranes from various brain areas with reducing agents, such as DTT, produced a concentration-dependent inhibition of ...

Abstract

Abstract: Treatment of the 2 adrenergic receptor with the reducing agent dithiothreitol (DTT) is known to abolish ligand binding to the receptor. Interestingly, the loss of binding can be prevented by preoccupation of the receptor with ligand. It is unclear, however, whether the ligand blocks access of DTT to the receptor, or the ligand stabilizes the receptor structure. In the present study, we have utilized circular dichroism (CD) and intrinsic tryptophan fluorescence to directly probe structural changes in the 2 adrenergic ...

Abstract

Recently, there has been a growing interest for wavelet frames corresponding to the union of an orthonormal wavelet basis and its dual Hilbert transformed wavelet basis. However, most of the existing works specifically address the dyadic case. In this paper, we consider orthonormal M-band wavelet decompositions, since we are motivated by their advantages in terms of frequency selectivity and symmetry of the analysis functions, for M > 2. More precisely, we establish phase conditions for a pair... ...

Abstract

We propose a 2D generalization to the M-band case of the dual-tree decomposition structure (initially proposed by N. Kingsbury and further investigated by I. Selesnick) based on a Hilbert pair of wavelets. We particularly address (i) the construction of the dual basis and (ii) the resulting directional analysis. We also revisit the necessary pre-processing stage in the M-band case. While several reconstructions are possible because of the redundancy of the representation, we propose a new... ...