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posted to aaf aaf-g af mismatch-repair mmr muts
by khobta
on 2012-11-06 12:17:07
Abstract
Defects in mismatch repair are associated with several types of cancer. It is also generally believed that environmental carcinogens are responsible for the initiation of cancers by the induction of mutations in critical genes. Prior genetic studies have suggested that the mismatch repair system can also recognize certain forms of DNA damage such as O6-methylguanine and UV photoproducts, and, therefore, mismatch repair may play a role in environmental agent-induced carcinogenesis. To examine this hypothesis, hMutSα, a heterodimer which consists of hMSH2 ...
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Vol. 83, No. 5. (1 September 1998), pp. 889-895
Abstract
BACKGROUND: Left and right colon carcinomas can display different clinical, pathologic, and genetic characteristics. The purpose of this study was to characterize multiple molecular genetic alterations in sporadic colon carcinoma and to correlate them with the location of the tumors and with lymph node metastasis. METHODS: One hundred and twenty-five cases of sporadic colon carcinoma (50 in the right colon and 75 in the left colon in patients with no family history of colon carcinoma) were studied. Status of the p53 ...
Note (first note only)
DA - 19980918
IS - 0008-543X (Print)
IS - 0008-543X (Linking)
LA - eng
PT - Journal Article
PT - Research Support, Non-U.S. Gov't
RN - 0 (Adaptor Proteins, Signal Transducing)
RN - 0 (Carrier Proteins)
RN - 0 (DNA-Binding Proteins)
RN - 0 (MLH1 protein, human)
RN - 0 (Neoplasm Proteins)
RN - 0 (Nuclear Proteins)
RN - 0 (Proto-Oncogene Proteins)
RN - EC 3.6.1.3 (MSH2 protein, human)
RN - EC 3.6.1.3 (MutS Homolog 2 Protein)
SB - AIM
SB - IM
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In Nucleic Acids Res, Vol. 26, No. 18. (1998), 4291-300
Abstract
The MutS protein of Escherichia coli plays a key role in the recognition and repair of errors made during the replication of DNA. Homologs of MutS have been found in many species including eukaryotes, Archaea and other bacteria, and together these proteins have been grouped into the MutS family. Although many of these proteins have similar activities to the E.coli MutS, there is significant diversity of function among the MutS family members. This diversity is even seen within species; many species ...
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Abstract
DNA adenine methylation by DNA adenine methyltransferase (Dam) in Escherichia coli plays an important role in processes such as DNA replication initiation, gene expression regulation, and mismatch repair. In addition, E. coli strains deficient in Dam are hypersensitive to DNA-damaging agents. We used genome microarrays to compare the transcriptional profiles of E. coli strains deficient in Dam and mismatch repair (dam, dam mutS, and mutS mutants). Our results show that >200 genes are expressed at a higher level in the dam ...
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The Journal of molecular diagnostics : JMD, Vol. 7, No. 4. (October 2005), pp. 465-77
by Julinor Bacani, Rhonda Zwingerman, Nando Di Nicola, et al.Samantha Spencer, Trish Wegrynowski, Kyle Mitchell, Kazuko Hay, Mark Redston, Eric Holowaty, David Huntsman, Aaron Pollett, Robert Riddell, Steven Gallinger
Abstract
Gastric cancer (GC) remains a leading cause of cancer mortality worldwide. Genetic factors are implicated, including DNA mismatch repair (MMR) deficiency manifested as tumor microsatellite instability (MSI). However, a standardized panel of markers and a definition of low-versus-high level MSI in GC are lacking. We examined a population-based cohort of early onset (<or=50 yrs) gastric cancer. We identified 211 cases of early onset gastric cancer in Central-East Ontario from 1989 to 1993, with archival material available for 139 cases. Testing included ...
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Abstract
The availability of inexpensive, on demand synthetic DNA has enabled numerous powerful applications in biotechnology, in turn driving considerable present interest in the de novo synthesis of increasingly longer DNA constructs. The synthesis of DNA from oligonucleotides into products even as large as small viral genomes has been accomplished. Despite such achievements, the costs and time required to generate such long constructs has, to date, precluded gene-length (and longer) DNA synthesis from being an everyday research tool in the same manner ...
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Abstract
Gene targeting provides a powerful means for analyzing gene function, as exemplified by knockout mouse studies and recent work with the highly recombinogenic chicken DT40 B-lymphocyte line. In human cultured cells, however, the low frequency of gene targeting is a serious barrier to efficiently generate knockout clones. Moreover, commonly used human cell lines are karyotypically abnormal or unstable. Here, we show using promoterless targeting constructs that Nalm-6, a human pre-B ALL cell line, is highly proficient for gene targeting by homologous ...
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posted to ber interaction mmr muts muty
by redondoself
on 2009-07-16 03:23:11
Abstract
Mismatch repair is the major pathway controlling genetic stability by removing mispairs caused by faulty replication and/or mismatches containing oxidized bases. Thus, inactivation of the Msh2 mismatch repair gene is associated with a mutator phenotype and increased cancer susceptibility. The base excision repair gene Mutyh is also involved in the maintenance of genomic integrity by repairing premutagenic lesions induced by oxidative DNA damage. Because evidence in bacteria suggested that Msh2 and Mutyh repair factors might have some overlapping functions, we investigated ...
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by Heather Hampel, Wendy L. Frankel, Edward Martin, et al.Mark Arnold, Karamjit Khanduja, Philip Kuebler, Mark Clendenning, Kaisa Sotamaa, Thomas Prior, Judith A. Westman, Jenny Panescu, Dan Fix, Janet Lockman, Jennifer Lajeunesse, Ilene Comeras, Albert
Abstract
PURPOSE: Identifying individuals with Lynch syndrome (LS) is highly beneficial. However, it is unclear whether microsatellite instability (MSI) or immunohistochemistry (IHC) should be used as the screening test and whether screening should target all patients with colorectal cancer (CRC) or those in high-risk subgroups. PATIENTS AND METHODS: MSI testing and IHC for the four mismatch repair proteins was performed on 500 tumors from unselected patients with CRC. If either MSI or IHC was abnormal, complete mutation analysis for the mismatch repair ...
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Nature Structural & Molecular Biology, Vol. 16, No. 5. (19 April 2009), pp. 550-557, doi:10.1038/nsmb.1596
Abstract
The Msh2Msh3 complex recognizes DNA mismatch lesions, with stronger affinity for small insertion and deletion loops. Now the nucleotide binding properties of Msh2Msh3 are studied, revealing the changes upon binding to DNA molecules with a loop lesion, indicating how this mismatch sensor can signal the repair machinery. ...
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Abstract
DNA adenine methylation by DNA adenine methyltransferase (Dam) in Escherichia coli plays an important role in processes such as DNA replication initiation, gene expression regulation, and mismatch repair. In addition, E. coli strains deficient in Dam are hypersensitive to DNA-damaging agents. We used genome microarrays to compare the transcriptional profiles of E. coli strains deficient in Dam and mismatch repair (dam, dam mutS, and mutS mutants). Our results show that >200 genes are expressed at a higher level in the dam ...
Note (first note only)
Oct
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Genetics, Vol. 155, No. 2. (Jun 2000), pp. 589-99
Abstract
In Saccharomyces cerevisiae, Msh2p, a central component in mismatch repair, forms a heterodimer with Msh3p to repair small insertion/deletion mismatches and with Msh6p to repair base pair mismatches and single-nucleotide insertion/deletion mismatches. In haploids, a msh2Delta mutation is synthetically lethal with pol3-01, a mutation in the Poldelta proofreading exonuclease. Six conditional alleles of msh2 were identified as those that conferred viability in pol3-01 strains at 26 degrees but not at 35 degrees. DNA sequencing revealed that mutations in several of the ...
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by Hong Wang, Yong Yang, Mark J. Schofield, et al.Chunwei Du, Yonatan Fridman, Susan D. Lee, Erik D. Larson, James T. Drummond, Eric Alani, Peggy Hsieh, Dorothy A. Erie
posted to afm dna mica mismatch muts
by ilyaf
on 2008-04-30 03:57:44
Abstract
DNA mismatch repair is central to the maintenance of genomic stability. It is initiated by the recognition of base-base mismatches and insertion/deletion loops by the family of MutS proteins. Subsequently, ATP induces a unique conformational change in the MutS-mismatch complex but not in the MutS-homoduplex complex that sets off the cascade of events that leads to repair. To gain insight into the mechanism by which MutS discriminates between mismatch and homoduplex DNA, we have examined the conformations of specific and nonspecific ...
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posted to afm dna mica mismatch muts
by ilyaf
on 2008-04-30 03:56:18
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Proc Natl Acad Sci USA, Vol. 98, No. 25. (Dec 2001), pp. 14607-12
Abstract
Among the several factors that affect the appearance and spread of acquired antibiotic resistance, the mutation frequency and the biological cost of resistance are of special importance. Measurements of the mutation frequency to rifampicin resistance in Helicobacter pylori strains isolated from dyspeptic patients showed that approximately 1/4 of the isolates had higher mutation frequencies than Enterobacteriaceae mismatch-repair defective mutants. This high mutation frequency could explain why resistance is so frequently acquired during antibiotic treatment of H. pylori infections. Inactivation of the ...
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posted to crystal mmr muts
by ecattell
on 2007-07-10 19:42:41
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Science; Science, Vol. 277, No. 5330. (Aug 1997), pp. 1259-1260
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Mol Cell Biol, Vol. 22, No. 7. (April 2002), pp. 2388-2397
posted to beta mmr muts
by ecattell
on 2005-10-23 22:55:08
Abstract
The removal of interstrand cross-links (ICLs) from DNA in higher eucaryotes is not well understood. Here, we show that processing of psoralen ICLs in mammalian cell extracts is dependent upon the mismatch repair complex hMutSbeta but is not dependent upon the hMutSalpha complex or hMlh1. The processing of psoralen ICLs is also dependent upon the nucleotide excision repair proteins Ercc1 and Xpf but not upon other components of the excision stage of this pathway or upon Fanconi anemia proteins. Products formed ...
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