Tag ptb [14 articles]

Abstract

We investigate a quadratic-quartic anharmonic oscillator formed by a potential well between two potential barriers. We realize this novel potential with a dc SQUID at near-zero current bias and flux bias near half a flux quantum. Escape out of the central well can occur via tunneling through either of the two barriers. We find good agreement with a generalized double-path macroscopic quantum tunneling theory. We also demonstrate an “optimal line” in current and flux bias along which the oscillator, which can ...

Abstract

We introduce a three-junction superconducting quantum interference device (SQUID) that can be used as an optimal tunable element in Josephson quantum computing applications. This device can replace the simple dc SQUID generally used as a tunable element in this kind of applications, with a series of advantages concerning the coherence time and the tolerance to small errors. We study the device both theoretically and experimentally at 4.2  K, obtaining a good agreement between the results. ...

Abstract

An all-superconductor charge qubit enabling a radio-frequency readout of its quantum state is described. The core element of the setup is a superconducting loop which includes the single-Cooper-pair (Bloch) transistor. This circuit has two functions: First, it operates as a charge qubit with magnetic control of Josephson coupling and electrostatic control of the charge on the transistor island. Secondly, it acts as the transducer of the rf electrometer, which probes the qubit state by measuring the Josephson inductance of the transistor. ...

Abstract

We propose a superconducting phase qubit on the basis of the radio-frequency superconducting quantum interference device with the screening parameter value β L ≡(2 π / Φ 0 ) L I c ≈1 , biased by a half-flux quantum Φ e = Φ 0 /2 . Significant anharmonicity (>30 % ) can be achieved in this system due to the interplay of the cosine Josephson potential and the parabolic magnetic energy potential that ultimately leads to the ...

Abstract

We have analyzed a radio-frequency single-electron-transistor (RF-SET) circuit that includes a high-electron-mobility-transistor (HEMT) amplifier, coupled to the single-electron-transistor (SET) via an impedance transformer. We consider how power is transferred between different components of the circuit, model noise components, and analyze the operating conditions of practical importance. The results are compared with experimental data on SETs. Good agreement is obtained between our noise model and the experimental results. Our analysis shows, also, that the biggest improvement to the present RF-SETs will be ...

Abstract

10.1261/rna.1821809 Many splicing factors interact with both mRNA and pre-mRNA. The identification of these interactions has been greatly improved by the development of in vivo cross-linking immunoprecipitation. However, the output carries a strong sampling bias in favor of RNPs that form on more abundant RNA species like mRNA. We have developed a novel in vitro approach for surveying binding on pre-mRNA, without cross-linking or sampling bias. Briefly, this approach entails specifically designed oligonucleotide pools that tile through a pre-mRNA sequence. The ...

Abstract

Polypyrimidine tract-binding protein (PTB) is a splicing regulator that also plays a positive role in pre-mRNA 3' end processing when bound upstream of the polyadenylation signal (pA signal). Here, we address the mechanism of PTB stimulatory function in mRNA 3' end formation. We identify PTB as the protein factor whose binding to the human beta-globin (HBB) 3' UTR is abrogated by a 3' end processing-inactivating mutation. We show that PTB promotes both in vitro 3' end cleavage and polyadenylation and recruits ...

Abstract

The polypyrimidine tract binding protein (PTB) is a 58-kDa RNA binding protein involved in multiple aspects of mRNA metabolism including splicing regulation, polyadenylation, 3'end formation, internal ribosomal entry site-mediated translation, RNA localization and stability. PTB contains four RNA recognition motifs (RRMs) separated by three linkers. In this review we summarize structural information on PTB in solution that has been gathered during the past 7 years using NMR spectroscopy and small-angle X-ray scattering. The structures of all RRMs of PTB in their ...

Abstract

BACKGROUND: Preterm birth causes 60% to 80% of neonatal deaths. Survivors can experience life-long complications. The risk of preterm labour in the presence of maternal infection is thought to be 30% to 50%. Probiotics are defined as live micro-organisms which, when administered in an adequate amount, confer a health benefit on the host. They have been shown to displace and kill pathogens and modulate the immune response by interfering with the inflammatory cascade that leads to preterm labour and delivery. During ...

Abstract

Many metazoan gene transcripts exhibit neuron-specific splicing patterns, but the developmental control of these splicing events is poorly understood. We show that the splicing of a large group of exons is reprogrammed during neuronal development by a switch in expression between two highly similar polypyrimidine tract-binding proteins, PTB and nPTB (neural PTB). PTB is a well-studied regulator of alternative splicing, but nPTB is a closely related paralog whose functional relationship to PTB is unknown. In the brain, nPTB protein is specifically ...

Abstract

RNA-binding motif protein 4 (RBM4) has been implicated in the regulation of precursor mRNA splicing. Using differential display analysis, we identified mRNAs that associate with RBM4-containing messenger RNPs in vivo. Among these mRNAs, alpha-tropomyosin (alpha-TM) is known to exhibit a muscle cell type-specific splicing pattern. The level of the skeletal muscle-specific alpha-TM mRNA isoform partially correlated with that of RBM4 in human tissues examined and could be modulated by ectopic overexpression or suppression of RBM4. These results indicated that RBM4 directly ...

Abstract

Polypyrimdine tract binding protein (PTB) is a regulator of alternative splicing, mRNA 3' end formation, mRNA stability and localization, and IRES-mediated translation. Transient overexpression of PTB can influence alternative splicing, sometimes resulting in nonphysiological splicing patterns. Here, we show that alternative skipping of PTB exon 11 leads to an mRNA that is removed by NMD and that this pathway consumes at least 20% of the PTB mRNA in HeLa cells. We also show that exon 11 skipping is itself promoted by ...

Abstract

Alternative pre-mRNA splicing is a central mode of genetic regulation in higher eukaryotes. Variability in splicing patterns is a major source of protein diversity from the genome. In this review, I describe what is currently known of the molecular mechanisms that control changes in splice site choice. I start with the best-characterized systems from the Drosophila sex determination pathway, and then describe the regulators of other systems about whose mechanisms there is some data. How these regulators are combined into complex ...