Abstract

The Visitors and Residents model of internet use suggests a continuum of modes of engagement with the online world, ranging from tool use to social spaces. In this paper, we examine evidence derived from a large cohort of students to assess whether this idea can be validated by experimental evidence. We find statistically significant differences between individuals displaying ?Visitor? or ?Resident? attitudes, suggesting that the Visitors and Residents model is a useful typology for approaching and understanding online behaviour. From our ...

Note (first note only)

Free version: https://lra.le.ac.uk/handle/2381/27783

Abstract

Whether or not you take a constructivist view of education, feedback on performance is inevitably seen as a crucial component of the process. However, experience shows that students (and academic staff) often struggle with feedback, which all too often fails to translate into feed-forward actions leading to educational gains. Problems get worse as student cohort sizes increase. By building on the well-established principle of separating marks from feedback and by using a social network approach to amplify peer discussion of assessed ...

Abstract

The impact of social networks on lives of the majority of young adults has been enormous, although their impact on education is less well understood. Some consideration has been give to the role Facebook plays in higher education and in the transition from secondary to tertiary education, but little analysis has been conducted on the role of the microblogging social network Twitter. By examining the use made of this service by two cohorts of students, this study found that Twitter is ...

Abstract

This paper describes a process of migration from formal, paper-based, institutionally owned processes towards informal, social, student-centred personal development. In terms of tool use, this journey involves moving from isolated personal silos to flexible online networks which attempts to use social tools to increase engagement with education. We describe here the evidence we have collected and analyzed which shows that social network portfolios allow powerful yet highly granular feedback loops and encourage the emergence of peer support and mentoring networks. The ...

Abstract

This guide will show you how you can use social media to help your research and your career. Social media have big implications for how researchers (and people in general) communicate and collaborate. Researchers have much to gain from engaging with social media in various aspects of their work. This guide will provide you with information to make an informed decision about using social media and enable you to select wisely from the vast range of tools ...

Abstract

Electronic publishing remains rooted in the print era while at the same time educational practice is moving beyond the "document-centric" approach. Google's new Wave technology shows one way in which new forms of publishing and interactive educational approaches might come together. ...

Abstract

‘Amplified events’ utilize social networks to record and relay discussions from a physical event to a much larger online audience. This case study is an analysis of the impact of Web 2.0 tools to amplify a local staff training event. We explore the affordances of social technologies to increase participation in the event beyond the confines of a single institution and give practical advice for others who would like to run an amplified event. ...

Abstract

The results are presented of a survey comparing the perceptions of first and second year bioscience students regarding their experience of feedback on coursework. The two cohorts displayed similar levels of satisfaction regarding the quantity and timing of feedback, even though changes in assessment format entailed different actual experiences. By contrast, the second year students expressed markedly lower levels of satisfaction regarding the guidance received in using feedback and the utility and transferability of the feedback received. These findings are discussed ...

Abstract

The results are presented of a survey comparing the perceptions of first and second year bioscience students regarding their experience of feedback on coursework. The two cohorts displayed similar levels of satisfaction regarding the quantity and timing of feedback, even though changes in assessment format entailed different actual experiences. By contrast, the second year students expressed markedly lower levels of satisfaction regarding the guidance received in using feedback and the utility and transferability of the feedback received. These findings are discussed ...

Abstract

Poster presented at Higher Education Academy Annual Conference from TechDis funded HEAT 3 project ...

Abstract

A wide range of tools is now available to enable teaching practitioners to create web-based educational materials from PowerPoint presentations, adding a variety of different digital media, such as audio and animation. The pilot study described in this paper compared three different systems for producing multimedia presentations from existing PowerPoint files. The resulting resources were tested by a group of disabled students and a group of non-disabled students. Our findings show that there were statistically significant differences between the two groups ...

Abstract

Feedback on academic performance is of critical importance to students’ learning, and in their perception of the quality of instruction they receive. Here we report the outcomes of a study comparing the views and expectations of first year biological science undergraduate students and academic staff regarding feedback provision and utilisation. The results indicate that while students and staff are generally satisfied with the feedback process, there are some tensions generated by perceived differences in desired academic outcomes. In particular, these tensions ...

Abstract

Sophisticated software such as Virtual Learning Environments (VLEs) are rapidly being deployed by universities. Despite widespread use of such systems, experience shows that there is frequently poor pedagogic development, leading primarily to use of VLEs as electronic document repositories rather than as online learning systems in which the available suite of tools are used to their full potential. Online assessment is the major potential efficiency gain of such systems, but most staff do not scratch the surface of the full capabilities ...

Abstract

Extended matching sets questions (EMSQs) are a form of multiple?choice question (MCQ) consisting of a stem (the question or scenario) with an extended number of possible answers. Although there is no consensus on their absolute format, for the purpose of this paper a multiple?choice question with ten or more alternative answers is considered to be an EMSQ. Faced with the limitations imposed by virtual learning environment software, I have conducted a case study into the use of the EMSQ format in ...

Abstract

Most Virtual Learning Environments are designed to be simple to use by staff and students alike. We report a case study where this feature has led academic staff to reject formal centralised training and teach themselves how to use the system. What has resulted is widespread use of the system but with poor pedagogic development, leading primarily to an electronic document repository rather than an online learning tool which makes full use of the potential of the full suite of available ...

Abstract

Numerical ability is an essential skill for everyone studying the biological sciences but many students are frightened by the 'perceived' difficulty of mathematics, and are nervous about applying mathematical skills in their chosen field of study. Having taught introductory maths and statistics for many years, Alan Cann understands these challenges and just how invaluable an accessible, confidence building textbook could be to the fearful student. Unable to find a book pitched at the right level, that concentrated on why numerical skills are useful to biologists, he wrote his ...

Abstract

A method to quantify double-stranded RNA-dependent protein kinase (PKR) mRNA and protein from human cells is described. A competitive RT-PCR assay has been developed by synthesis of an internal standard control (ISC) species of RNA. A competitive immunoblot assay was used to quantify full-length PKR (FL-PKR) protein in a sample of total cellular proteins, using truncated PKR protein as an internal standard against which FL-PKR protein could be quantified. The method can be used for simultaneous analysis of transcriptional and postranscriptional ...

Abstract

Overexpression of P-glycoprotein (P-gp), the protein product of the multidrug resistance gene (MDR1), confers a drug resistant phenotype on cells. This phenotype is reminiscent of human T-cell leukemia virus (HTLV)-transformed leukemic cells, for which no consistently effective chemotherapeutic regime has been found. The presence of an active multiple drug resistance (MDR) phenotype in freshly isolated peripheral blood mononuclear cells (PBMC) from HTLV-I-infected subjects was investigated. Significant P-gp-mediated efflux activity and enhanced MDR1 mRNA expression was observed in nine of 10 HTLV-infected ...

Abstract

Overexpression of P-glycoprotein (P-gp), the protein product of the multidrug resistance gene (MDR1), confers a drug resistant phenotype on cells. We have recently demonstrated that the MDR1 promoter is transcriptionally activated by the HTLV-I tax protein, providing an explanation for the development of drug resistance in HTLV-I infections. Here we report that HTLV-I mediated MDR1 activation is dependent on the presence of an NF-IL6-binding site located between base pairs -148 and -141 relative to the transcription start site. This finding opens ...

Abstract

Genomic RNA isolated from retroviral particles is a dimer composed of two identical strands. A region called the dimer linkage signal close to the 5' end of the RNA may be involved in forming the dimer. Several models for the formation of the HIV-1 RNA dimer have been proposed. In the kissing loop model, dimerisation results from base-pairing between homologous sequences in an RNA stem-loop. In the guanine tetrad model interstrand guanine contacts from the dimer. We have made mutations preventing ...

Abstract

A recombinant vector that rapidly produces large amounts of human immunodeficiency virus (HIV) virus-like particles (VLPs) was constructed. This vector lacks LTR sequences and a functional nef gene. The VLPs produced are non-infectious but similar in structure to mature, infectious HIV virions. They package specifically HIV RNAs containing appropriate signals and do not package abundant cellular mRNAs (e.g. actin). In the system described here, efficient particle production and release is decoupled from infection. Use of this VLP system offers many advantages ...

Abstract

We and others have identified the major determinant of cell tropism in human immunodeficiency virus type 1 (HIV-1) as the V3 loop of glycoprotein gp120. We have conducted a detailed study of two molecularly cloned isolates of HIV-1, HIVJR-CSF and HIVNL4-3, that differ in their tropism for immortalized CD4+ cell lines, by constructing a series of site-directed mutations within the V3 loop of HIVJR-CSF based on the sequence of HIVNL4-3. The phenotypes of these mutants fall into two classes, those which ...

Abstract

Specific point mutations which affect viral tropism have been identified in both the V3 loop and in the CD4-binding region of the human immunodeficiency virus type 1 surface glycoprotein gp120. Here we report that a single point mutation in the first variable region (V1) of human immunodeficiency virus type 1 strain JRCSF is responsible for a change in viral tropism. ...

Abstract

Numerous studies implicate cellular immunological effector systems in the partial containment of virus replication during the early stages of HIV infection. Immunostimulatory therapeutic regimes designed to enhance virus clearance are therefore theoretically attractive, but are accompanied by the risk of concomitant activation of HIV replication. Supra-normal levels of L-arginine have been shown to induce broad immune stimulation in vitro and in vivo, but do not increase HIV gene expression in vitro. These observations, together with the lack of toxicity of this ...

Abstract

In the 10 years since AIDS was first identified, knowledge of the causative agent, human immunodeficiency virus (HIV), has advanced remarkably. Molecular biological analysis has had a greater impact on the investigation of HIV and AIDS than on that of any other disease. Unfortunately, the vast amount of material published on this subject has still not resulted in a thorough understanding of the pathogenesis of AIDS. Undoubtedly, part of the reason for the complexity of this disease stems from the ability ...

Abstract

Different isolates of human immunodeficiency virus type 1 (HIV-1) vary in the cell tropisms they display, i.e., the range of cell types in which they are able to establish a productive infection. Here, we report on the phenotypes of recombinants between two molecularly cloned strains of HIV-1. Our results prove that the envelope glycoprotein gp120 is solely responsible for the difference in cell tropism between the two parental isolates and that no other genes or sequences are involved in determining the ...

Abstract

Infectious recombinant viruses were constructed from three molecularly cloned human immunodeficiency virus (HIV) strains varying in cell tropism. All recombinants showed a high infectivity titer on phytohemagglutinin-stimulated normal T lymphocytes. However, a 120-bp region of the envelope gene including the area of the V3 hypervariable loop was found to influence infectivity titer on both clone 1022 CD4-positive HeLa cells and CD4-positive CEM leukemia cells. Infectivity for macrophages was more complex. All viruses replicated in macrophages to a low level, but viral ...

Abstract

Different strains of human immunodeficiency virus type 1 (HIV-1) vary in the ability to replicate in cells that bear the HIV-1 receptor, CD4. The mechanism responsible for these cell tropism differences is unknown. We examined different isolates of HIV-1 with regard to replication in specific tumor-derived CD4-positive T-cell lines and normal peripheral blood lymphocytes. To investigate early events in the virus life cycle at low multiplicities of infection, we used a modification of the polymerase chain reaction method. Use of a ...

Abstract

The tax gene of the human T-cell leukemia virus types I and II (HTLV-I and HTLV-II) is essential for viral replication and acts by increasing the level of RNA transcription. The tax genes of HTLV-I and HTLV-II encode proteins of 40 and 37 kilodaltons, respectively. By in vitro mutagenesis of the tax gene, we have investigated those regions of the protein which are essential for its function. Mutation of either the amino- or carboxy-terminal domain of the protein resulted in loss ...

Abstract

The tax protein of HTLV-II increases the level of steady-state mRNA produced from the HTLV-II long terminal repeat (LTR) and also activates heterologous promoters. We have previously shown that the adenovirus E3 promoter, which is trans-activated by the adenovirus E1a protein, is also trans-activated by the tax protein. To investigate the mechanism of trans-activation by tax, we analyzed E3 promoter deletion mutants to determine nucleotide sequence requirements for activation of this promoter. Our results show that removal of different upstream regions ...

Abstract

The human T-cell leukemia virus (HTLV) types I and II have two nonstructural genes that are encoded in overlapping reading frames. One of these genes, known as tax, has been shown to encode a protein responsible for enhanced transcription (transactivation) from the viral long terminal repeats (LTRs). Genetic evidence indicates that the second nonstructural gene of HTLV-II, here designated rex, acts in trans to modulate tax gene-mediated transactivation in a concentration-dependent fashion. The rex gene may regulate the process of transactivation ...

Abstract

The human immunodeficiency virus type 1 (HIV-1) and human T-cell leukemia virus type I (HTLV-I) are two distinct human retroviruses that infect T cells. Recent epidemiologic studies have identified a cohort of individuals that are coinfected with both viruses. It is reported here that human peripheral blood leukocytes infected with HIV-1 in vitro can be induced to produce large quantities of HIV-1 after mitogenic stimulation by noninfectious HTLV-I virions. It is also shown that HTLV-I virions may exert this effect prior ...

Abstract

Human T-cell leukemia virus (HTLV) types I and II are unusual among replication-competent retroviruses in that they contain a fourth gene (chi) necessary for replication. The chi gene product, p chi, transcriptionally transactivates the viral long repeat (LTR), and is thus a positive regulator. To investigate p chi transactivation, sequences from the U3 regions of the LTRs of HTLV-I and -II were inserted into the Moloney murine leukemia virus (M-MuLV) LTR by recombinant DNA techniques. Transient expression assays of the chimeric ...

Abstract

The human T-cell leukemia viruses, HTLV-I and HTLV-II, contain a gene, termed x, with transcriptional regulatory function. The properties of the x proteins were analyzed by constructing mutant genes containing site-directed deletions and point mutations. The results demonstrate that the amino terminal 17 amino acids of the x protein constitute part of a functional domain that is critical for the transcriptional activating properties of the protein. Within this region, substitution of a leucine residue for a proline residue results in major ...

Abstract

The mechanism of cellular transformation by the human T-cell leukemia viruses (HTLVs) is thought to involve a novel retrovirus gene known as chi. The chi gene is essential for HTLV replication and acts by enhancing transcription from the viral long terminal repeat. By using the HTLV type I and II chi gene-coding regions inserted into a highly efficient expression vector, we directly compared the efficiencies of the two chi proteins to trans activate the HTLV type I and II long terminal ...