Bacterial turgor pressure can be measured by atomic force microscopy Export

@article{M.Arnoldi.2000, abstract = {We report a study of the deformability of a bacterial wall with an atomic force microscope (AFM). A theoretical expression is derived for the force exerted by the wall on the cantilever as a function of the depths of indentation generated by the AFM tip. Evidence is provided that this reaction force is a measure for the turgor pressure of the bacterium. The method was applied to magnetotactic bacteria of the species Magnetospirillum gryphiswaldense . Force curves were generated on the substrate and on the bacteria while scanning laterally. With the mechanical properties so gained we obtained the spring constant of the bacterium as a whole. Making use of our theoretical results we determined the turgor pressure to be in the range of 85 to 150 kPa.}, author = {Arnoldi, Markus and Fritz, Monika and B\"{a}uerlein, Edmund and Radmacher, Manfred and Sackmann, Erich and Boulbitch, Alexei}, citeulike-article-id = {4154577}, citeulike-linkout-0 = {http://dx.doi.org/10.1103/PhysRevE.62.1034}, citeulike-linkout-1 = {http://link.aps.org/abstract/PRE/v62/i1/p1034_1}, citeulike-linkout-2 = {http://link.aps.org/pdf/PRE/v62/i1/p1034_1}, day = {1}, doi = {10.1103/PhysRevE.62.1034}, journal = {Physical Review E}, keywords = {afm, atomic\_force\_microscopy, bacteria, cell, cell\_wall\_structure, indentation, mechanical\_properties, theoretical, turgor\_pressure}, month = {Jul}, number = {1}, pages = {1034--1044}, posted-at = {2009-11-06 15:22:37}, priority = {2}, publisher = {American Physical Society}, title = {Bacterial turgor pressure can be measured by atomic force microscopy}, url = {http://dx.doi.org/10.1103/PhysRevE.62.1034}, volume = {62}, year = {2000} }

TY - JOUR ID - M.Arnoldi.2000 L3 - citeulike-article-id:4154577 N2 - We report a study of the deformability of a bacterial wall with an atomic force microscope (AFM). A theoretical expression is derived for the force exerted by the wall on the cantilever as a function of the depths of indentation generated by the AFM tip. Evidence is provided that this reaction force is a measure for the turgor pressure of the bacterium. The method was applied to magnetotactic bacteria of the species Magnetospirillum gryphiswaldense . Force curves were generated on the substrate and on the bacteria while scanning laterally. With the mechanical properties so gained we obtained the spring constant of the bacterium as a whole. Making use of our theoretical results we determined the turgor pressure to be in the range of 85 to 150 kPa. IS - 1 JF - Physical Review E EP - 1044 TI - Bacterial turgor pressure can be measured by atomic force microscopy PB - American Physical Society VL - 62 SP - 1034 KW - afm KW - atomic_force_microscopy KW - bacteria KW - cell KW - cell_wall_structure KW - indentation KW - mechanical_properties KW - theoretical KW - turgor_pressure AU - Arnoldi, Markus AU - Fritz, Monika AU - Bäuerlein, Edmund AU - Radmacher, Manfred AU - Sackmann, Erich AU - Boulbitch, Alexei PY - 2000/Jul/01/ UR - http://dx.doi.org/10.1103/PhysRevE.62.1034 ER -