Lamina propria macrophages and dendritic cells differentially induce regulatory and interleukin 17-producing T cell responses Export

@article{citeulike:1672966, author = {Denning, Timothy L. and Wang, Yi-Chong and Patel, Seema R. and Williams, Ifor R. and Pulendran, Bali}, citeulike-article-id = {1672966}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/ni1511}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/ni1511}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17873879}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17873879}, comment = {LP macrophages (CD11b+ CD11c low/neg)produce IL-10 +/- TLR stimulation and only when stimulated with CpG they produce some IL-12p40. TLR-stimulated splenic macrophages produced more IL-10 than IL-12p40 (except when stimulated with CpG) ---=note-separator=--- CD11b+ CD11c -/low LP cells cultured with OTII and TGFb (4d) induce high\% of Foxp3+CD4+ cells (37\%, splenic macs 5.6\%)the differentiation was reverted when anti-IL-10+antiIL-10R were added to the plate Addition of IL+6 also reverts foxp3+CD4+ differentiation. LP DCs (CD11b+ or CD11b-) did not induce Foxp3+ CD4+ cells (0.4 and 0.8\%) ---=note-separator=--- Culturing together LP CD11b+ CD11c neg/low + CD11c+DCs reduced Th17 differentiation as compared with DCs alone (from 10\% to 1.4\%) Th1 differentiation was increased (6.8 to 12\%) ---=note-separator=--- Like splenic macrophages, lamina propria CD11b+CD11c- cells did not express the lineage markers CD4, CD8alpha, TCRbeta, CD19, B220 and NK1.1, suggesting that they were not T cells, B cells or natural killer cells (Supplementary Fig. 1 online). In addition, lamina propria CD11b+CD11c- cells lacked the DC markers DEC-205 and 33D1, as well as the Flt3 receptor CD135. However, relative to their splenic counterparts, lamina propria CD11b+CD11c- cells had high expression of the macrophage marker F4/80, the class II major histocompatibility complex molecule I-Ab, and the coinhibitory molecule programmed death ligand 1 (PD-L1; Fig. 1d). In addition, lamina propria CD11b+CD11c- cells had negligible expression of Gr-1 (data not shown) and CD62L (Fig. 1d). These results collectively suggest that lamina propria CD11b+CD11c- cells are distinct from previously reported lamina propria DCs and probably represent an abundant population of macrophages.}, day = {16}, doi = {10.1038/ni1511}, issn = {1529-2908}, journal = {Nat Immunol}, keywords = {macrophages-intestine, mucosal-immunology}, month = {October}, number = {10}, pages = {1086--1094}, posted-at = {2008-07-29 02:33:36}, priority = {2}, publisher = {Nature Publishing Group}, title = {Lamina propria macrophages and dendritic cells differentially induce regulatory and interleukin 17-producing T cell responses}, url = {http://dx.doi.org/10.1038/ni1511}, volume = {8}, year = {2007} }

TY - JOUR ID - citeulike:1672966 L3 - citeulike-article-id:1672966 IS - 10 JF - Nat Immunol SN - 1529-2908 EP - 1094 TI - Lamina propria macrophages and dendritic cells differentially induce regulatory and interleukin 17-producing T cell responses PB - Nature Publishing Group VL - 8 SP - 1086 KW - macrophages-intestine KW - mucosal-immunology N1 - LP macrophages (CD11b+ CD11c low/neg)produce IL-10 +/- TLR stimulation and only when stimulated with CpG they produce some IL-12p40. TLR-stimulated splenic macrophages produced more IL-10 than IL-12p40 (except when stimulated with CpG) N1 - CD11b+ CD11c -/low LP cells cultured with OTII and TGFb (4d) induce high% of Foxp3+CD4+ cells (37%, splenic macs 5.6%)the differentiation was reverted when anti-IL-10+antiIL-10R were added to the plate Addition of IL+6 also reverts foxp3+CD4+ differentiation. LP DCs (CD11b+ or CD11b-) did not induce Foxp3+ CD4+ cells (0.4 and 0.8%) N1 - Culturing together LP CD11b+ CD11c neg/low + CD11c+DCs reduced Th17 differentiation as compared with DCs alone (from 10% to 1.4%) Th1 differentiation was increased (6.8 to 12%) N1 - Like splenic macrophages, lamina propria CD11b+CD11c- cells did not express the lineage markers CD4, CD8alpha, TCRbeta, CD19, B220 and NK1.1, suggesting that they were not T cells, B cells or natural killer cells (Supplementary Fig. 1 online). In addition, lamina propria CD11b+CD11c- cells lacked the DC markers DEC-205 and 33D1, as well as the Flt3 receptor CD135. However, relative to their splenic counterparts, lamina propria CD11b+CD11c- cells had high expression of the macrophage marker F4/80, the class II major histocompatibility complex molecule I-Ab, and the coinhibitory molecule programmed death ligand 1 (PD-L1; Fig. 1d). In addition, lamina propria CD11b+CD11c- cells had negligible expression of Gr-1 (data not shown) and CD62L (Fig. 1d). These results collectively suggest that lamina propria CD11b+CD11c- cells are distinct from previously reported lamina propria DCs and probably represent an abundant population of macrophages. AU - Denning, Timothy AU - Wang, Yi-Chong AU - Patel, Seema AU - Williams, Ifor AU - Pulendran, Bali PY - 2007/10/16/ UR - http://dx.doi.org/10.1038/ni1511 ER -