Carbon nutrition of Escherichia coli in the mouse intestine. Export

@article{citeulike:3848154, abstract = {Whole-genome expression profiling revealed Escherichia coli MG1655 genes induced by growth on mucus, conditions designed to mimic nutrient availability in the mammalian intestine. Most were nutritional genes corresponding to catabolic pathways for nutrients found in mucus. We knocked out several pathways and tested the relative fitness of the mutants for colonization of the mouse intestine in competition with their wild-type parent. We found that only mutations in sugar pathways affected colonization, not phospholipid and amino acid catabolism, not gluconeogenesis, not the tricarboxylic acid cycle, and not the pentose phosphate pathway. Gluconate appeared to be a major carbon source used by E. coli MG1655 to colonize, having an impact on both the initiation and maintenance stages. N-acetylglucosamine and N-acetylneuraminic acid appeared to be involved in initiation, but not maintenance. Glucuronate, mannose, fucose, and ribose appeared to be involved in maintenance, but not initiation. The in vitro order of preference for these seven sugars paralleled the relative impact of the corresponding metabolic lesions on colonization: gluconate > N-acetylglucosamine > N-acetylneuraminic acid = glucuronate > mannose > fucose > ribose. The results of this systematic analysis of nutrients used by E. coli MG1655 to colonize the mouse intestine are intriguing in light of the nutrient-niche hypothesis, which states that the ecological niches within the intestine are defined by nutrient availability. Because humans are presumably colonized with different commensal strains, differences in nutrient availability may provide an open niche for infecting E. coli pathogens in some individuals and a barrier to infection in others.}, author = {Chang, D. E. and Smalley, D. J. and Tucker, D. L. and Leatham, M. P. and Norris, W. E. and Stevenson, S. J. and Anderson, A. B. and Grissom, J. E. and Laux, D. C. and Cohen, P. S. and Conway, T.}, citeulike-article-id = {3848154}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0307888101}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15123798}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15123798}, day = {11}, doi = {10.1073/pnas.0307888101}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {ibd, mucosal-immunology}, month = {May}, number = {19}, pages = {7427--7432}, posted-at = {2009-01-04 22:35:27}, priority = {2}, title = {Carbon nutrition of Escherichia coli in the mouse intestine.}, url = {http://dx.doi.org/10.1073/pnas.0307888101}, volume = {101}, year = {2004} }

TY - JOUR ID - citeulike:3848154 L3 - citeulike-article-id:3848154 N2 - Whole-genome expression profiling revealed Escherichia coli MG1655 genes induced by growth on mucus, conditions designed to mimic nutrient availability in the mammalian intestine. Most were nutritional genes corresponding to catabolic pathways for nutrients found in mucus. We knocked out several pathways and tested the relative fitness of the mutants for colonization of the mouse intestine in competition with their wild-type parent. We found that only mutations in sugar pathways affected colonization, not phospholipid and amino acid catabolism, not gluconeogenesis, not the tricarboxylic acid cycle, and not the pentose phosphate pathway. Gluconate appeared to be a major carbon source used by E. coli MG1655 to colonize, having an impact on both the initiation and maintenance stages. N-acetylglucosamine and N-acetylneuraminic acid appeared to be involved in initiation, but not maintenance. Glucuronate, mannose, fucose, and ribose appeared to be involved in maintenance, but not initiation. The in vitro order of preference for these seven sugars paralleled the relative impact of the corresponding metabolic lesions on colonization: gluconate > N-acetylglucosamine > N-acetylneuraminic acid = glucuronate > mannose > fucose > ribose. The results of this systematic analysis of nutrients used by E. coli MG1655 to colonize the mouse intestine are intriguing in light of the nutrient-niche hypothesis, which states that the ecological niches within the intestine are defined by nutrient availability. Because humans are presumably colonized with different commensal strains, differences in nutrient availability may provide an open niche for infecting E. coli pathogens in some individuals and a barrier to infection in others. IS - 19 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 0027-8424 EP - 7432 TI - Carbon nutrition of Escherichia coli in the mouse intestine. VL - 101 SP - 7427 KW - ibd KW - mucosal-immunology AU - Chang, DE AU - Smalley, DJ AU - Tucker, DL AU - Leatham, MP AU - Norris, WE AU - Stevenson, SJ AU - Anderson, AB AU - Grissom, JE AU - Laux, DC AU - Cohen, PS AU - Conway, T PY - 2004/05/11/ UR - http://dx.doi.org/10.1073/pnas.0307888101 ER -