Effects of administration of progenipoietin 1, Flt-3 ligand, granulocyte colony-stimulating factor, and pegylated granulocyte-macrophage colony-stimulating factor on dendritic cell subsets in mice Export

@article{citeulike:4130773, abstract = {We studied the effects of administration of several cytokines, including progenipoietin-1 (ProGP-1), Flt-3 ligand (FL), granulocyte colony-stimulating factor (G-CSF), and granulocyte-macrophage colony-stimulating factor in a pegylated form (pGM-CSF), on dendritic cell (DC) populations in mouse spleen. ProGP-1 produced the most striking increase in overall DC numbers, apparently more than its constituent FL and G-CSF components. However, the expansion in DC numbers was strongly subpopulation selective, with ProGP-1 and FL producing selective expansion of CD8+ DCs, whereas pGM-CSF produced selective expansion of CD8[-] DCs. Surprising differences were observed between the effects of murine and human recombinant FL preparations on murine DCs. Many of the biologic functions of the DC subpopulations expanded by cytokines remained intact, including the capacity of the ProGP-1- and FL-expanded CD8+ DCs to produce the T-helper-1-biasing cytokine interleukin 12 (IL-12). However, the expanded DCs from all but G-CSF-treated mice were deficient in the ability to make interferon [gamma], and the CD8+ DCs produced with pGM-CSF treatment had an abrogated capacity to form bioactive IL-12. Such selective expansion of DC populations and alterations in their cytokine-secretion capacity have implications for clinical use of the studied cytokines in immune modulation. 10.1182/blood.V99.6.2122}, author = {O'Keeffe, Meredith and Hochrein, Hubertus and Vremec, David and Pooley, Joanne and Evans, Robert and Woulfe, Susan and Shortman, Ken}, citeulike-article-id = {4130773}, citeulike-linkout-0 = {http://dx.doi.org/10.1182/blood.V99.6.2122}, citeulike-linkout-1 = {http://bloodjournal.hematologylibrary.org/cgi/content/abstract/99/6/2122}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/11877288}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=11877288}, day = {15}, doi = {10.1182/blood.V99.6.2122}, journal = {Blood}, keywords = {b16flt3, basic-dcs, intestinal-dcs}, month = {March}, number = {6}, pages = {2122--2130}, posted-at = {2009-03-04 00:38:44}, priority = {2}, title = {Effects of administration of progenipoietin 1, Flt-3 ligand, granulocyte colony-stimulating factor, and pegylated granulocyte-macrophage colony-stimulating factor on dendritic cell subsets in mice}, url = {http://dx.doi.org/10.1182/blood.V99.6.2122}, volume = {99}, year = {2002} }

TY - JOUR ID - citeulike:4130773 L3 - citeulike-article-id:4130773 N2 - We studied the effects of administration of several cytokines, including progenipoietin-1 (ProGP-1), Flt-3 ligand (FL), granulocyte colony-stimulating factor (G-CSF), and granulocyte-macrophage colony-stimulating factor in a pegylated form (pGM-CSF), on dendritic cell (DC) populations in mouse spleen. ProGP-1 produced the most striking increase in overall DC numbers, apparently more than its constituent FL and G-CSF components. However, the expansion in DC numbers was strongly subpopulation selective, with ProGP-1 and FL producing selective expansion of CD8+ DCs, whereas pGM-CSF produced selective expansion of CD8[-] DCs. Surprising differences were observed between the effects of murine and human recombinant FL preparations on murine DCs. Many of the biologic functions of the DC subpopulations expanded by cytokines remained intact, including the capacity of the ProGP-1- and FL-expanded CD8+ DCs to produce the T-helper-1-biasing cytokine interleukin 12 (IL-12). However, the expanded DCs from all but G-CSF-treated mice were deficient in the ability to make interferon [gamma], and the CD8+ DCs produced with pGM-CSF treatment had an abrogated capacity to form bioactive IL-12. Such selective expansion of DC populations and alterations in their cytokine-secretion capacity have implications for clinical use of the studied cytokines in immune modulation. 10.1182/blood.V99.6.2122 IS - 6 JF - Blood EP - 2130 TI - Effects of administration of progenipoietin 1, Flt-3 ligand, granulocyte colony-stimulating factor, and pegylated granulocyte-macrophage colony-stimulating factor on dendritic cell subsets in mice VL - 99 SP - 2122 KW - b16flt3 KW - basic-dcs KW - intestinal-dcs AU - O'Keeffe, Meredith AU - Hochrein, Hubertus AU - Vremec, David AU - Pooley, Joanne AU - Evans, Robert AU - Woulfe, Susan AU - Shortman, Ken PY - 2002/03/15/ UR - http://dx.doi.org/10.1182/blood.V99.6.2122 ER -