Induction of oral tolerance to cellular immune responses in the absence of Peyer's patches Export

@article{citeulike:4462223, abstract = {Systemic hyporesponsiveness occurs following oral administration of antigen (oral tolerance) and involves the uptake and processing of antigen by the gut-associated lymphoid tissue (GALT), which includes Peyer's patches (PP) lamina propria lymphocytes and mesenteric lymph nodes (MLN). Animals with targeted mutations of genes in the tumor necrosis factor (TNF) family have differential defects in the development of peripheral lymphoid organs including PP and MLN, and provide a unique opportunity to investigate the role of GALT structures in the induction of oral tolerance. Oral tolerance could not be induced in TNF/lymphotoxin (LT) \&agr;-/- mice, which are devoid of both PP and MLN, although these animals could be tolerized by intraperitoneal administration of antigen, demonstrating the requirement for GALT for oral tolerance induction. LT\&bgr;-/- mice and LT\&agr;/LT\&bgr;+/- animals do not have PP but could be orally tolerized, as measured by IFN-\&ggr; production and delayed-type hypersensitivity responses by administration of both low or high doses of ovalbumin. To further investigate the requirement for PP, we tested the progeny of LT\&bgr;-receptor-IgG-fusion-protein (LT\&bgr;RigG)-treated mice, which do not form PP but have an otherwise intact immune system. Although these animals had decreased fecal IgA production, they could be orally tolerized. Our results demonstrate that PP are not an absolute requirement for the induction of either high- or low-dose oral tolerance, although oral tolerance could not be induced in animals devoid of both PP and MLN.}, address = {Center for Neurologic Diseases, Brigham and Women's Hospital and Harvard Medical School, Boston, USA; Section of Clinical Immunology, Department of Internal Medicine, University Hospital Z\"{u}rich, Z\"{u}rich, Switzerland; Howard Hughes Medical Institute and Section of Immunobiology, Yale University School of Medicine, New Haven, USA; Department of Epidemiology and Public Health and Section of Immunobiology, Yale University School of Medicine, New Haven, USA; Biogen, 12 Cambridge Center, Cambridge, USA}, author = {Thomas w and Fontana, Adriano and Ana m and Anthony j and Eugster, Hans-Pietro and Zhang, Xingmin and Pandelakis a and Nancy h and Richard a and Paul d and Howard l}, citeulike-article-id = {4462223}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/1521-4141(200104)31:4%3C1278::AID-IMMU1278%3E3.0.CO;2-A}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/78503910/ABSTRACT}, doi = {10.1002/1521-4141(200104)31:4%3C1278::AID-IMMU1278%3E3.0.CO;2-A}, journal = {European Journal of Immunology}, keywords = {oral-tolerance}, number = {4}, pages = {1278--1287}, posted-at = {2009-05-04 19:00:52}, priority = {2}, title = {Induction of oral tolerance to cellular immune responses in the absence of Peyer's patches}, url = {http://dx.doi.org/10.1002/1521-4141(200104)31:4%3C1278::AID-IMMU1278%3E3.0.CO;2-A}, volume = {31}, year = {2001} }

TY - JOUR ID - citeulike:4462223 L3 - citeulike-article-id:4462223 N2 - Systemic hyporesponsiveness occurs following oral administration of antigen (oral tolerance) and involves the uptake and processing of antigen by the gut-associated lymphoid tissue (GALT), which includes Peyer's patches (PP) lamina propria lymphocytes and mesenteric lymph nodes (MLN). Animals with targeted mutations of genes in the tumor necrosis factor (TNF) family have differential defects in the development of peripheral lymphoid organs including PP and MLN, and provide a unique opportunity to investigate the role of GALT structures in the induction of oral tolerance. Oral tolerance could not be induced in TNF/lymphotoxin (LT) &agr;-/- mice, which are devoid of both PP and MLN, although these animals could be tolerized by intraperitoneal administration of antigen, demonstrating the requirement for GALT for oral tolerance induction. LT&bgr;-/- mice and LT&agr;/LT&bgr;+/- animals do not have PP but could be orally tolerized, as measured by IFN-&ggr; production and delayed-type hypersensitivity responses by administration of both low or high doses of ovalbumin. To further investigate the requirement for PP, we tested the progeny of LT&bgr;-receptor-IgG-fusion-protein (LT&bgr;RigG)-treated mice, which do not form PP but have an otherwise intact immune system. Although these animals had decreased fecal IgA production, they could be orally tolerized. Our results demonstrate that PP are not an absolute requirement for the induction of either high- or low-dose oral tolerance, although oral tolerance could not be induced in animals devoid of both PP and MLN. IS - 4 JF - European Journal of Immunology AD - Center for Neurologic Diseases, Brigham and Women's Hospital and Harvard Medical School, Boston, USA; Section of Clinical Immunology, Department of Internal Medicine, University Hospital Zürich, Zürich, Switzerland; Howard Hughes Medical Institute and Section of Immunobiology, Yale University School of Medicine, New Haven, USA; Department of Epidemiology and Public Health and Section of Immunobiology, Yale University School of Medicine, New Haven, USA; Biogen, 12 Cambridge Center, Cambridge, USA EP - 1287 TI - Induction of oral tolerance to cellular immune responses in the absence of Peyer's patches VL - 31 SP - 1278 KW - oral-tolerance AU - Thomas w AU - Fontana, Adriano AU - Ana m AU - Anthony j AU - Eugster, Hans-Pietro AU - Zhang, Xingmin AU - Pandelakis a AU - Nancy h AU - Richard a AU - Paul d AU - Howard l PY - 2001/// UR - http://dx.doi.org/10.1002/1521-4141(200104)31:4%3C1278::AID-IMMU1278%3E3.0.CO;2-A ER -