T Helper 1 and T Helper 2 Cells Are Pathogenic in an Antigen-specific Model of Colitis Export

@article{citeulike:5014590, abstract = {Dysregulated T cell responses to enteric bacteria have been implicated as a common mechanism underlying pathogenesis in rodent models of colitis. However, the bacterial species and T cell specificities that induce disease have been poorly defined. We have developed a model system in which target antigen, bacterial host, and corresponding T cell specificity are defined. OVA-specific T cells from DO11.RAG-2-/- TCR transgenic mice were transferred into RAG-2-/- recipients whose intestinal tracts were colonized with OVA-expressing or control Escherichia coli. Transfer of antigen-naive DO11.RAG-2-/- T cells into recipients colonized with OVA-E. coli resulted in enhanced intestinal recruitment and cell cycling of OVA-specific T cells; however, there was no development of disease. In contrast, transfer of polarized T helper (Th) 1 and Th2 populations resulted in severe wasting and colitis in recipients colonized with OVA-expressing but not control E. coli. The histopathologic features of disease induced by Th1 and Th2 transfers were distinct, but disease severity was comparable. Induction of disease by both Th1 and Th2 transfers was dependent on bacterially associated OVA. These results establish that a single bacterially associated antigen can drive the progression of colitis mediated by both Th1 and Th2 cells and provide a new model for understanding the immunoregulatory interactions between T cells responsive to gut floral antigens. 10.1084/jem.2001889}, author = {Iqbal, Nuzhat and Oliver, James R. and Wagner, Frederic H. and Lazenby, Audrey S. and Elson, Charles O. and Weaver, Casey T.}, citeulike-article-id = {5014590}, citeulike-linkout-0 = {http://dx.doi.org/10.1084/jem.2001889}, citeulike-linkout-1 = {http://jem.rupress.org/cgi/content/abstract/195/1/71}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/11781367}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=11781367}, doi = {10.1084/jem.2001889}, journal = {J. Exp. Med.}, keywords = {ecoli, ibd}, month = {January}, number = {1}, pages = {71--84}, posted-at = {2009-06-29 18:10:04}, priority = {2}, title = {T Helper 1 and T Helper 2 Cells Are Pathogenic in an Antigen-specific Model of Colitis}, url = {http://dx.doi.org/10.1084/jem.2001889}, volume = {195}, year = {2002} }

TY - JOUR ID - citeulike:5014590 L3 - citeulike-article-id:5014590 N2 - Dysregulated T cell responses to enteric bacteria have been implicated as a common mechanism underlying pathogenesis in rodent models of colitis. However, the bacterial species and T cell specificities that induce disease have been poorly defined. We have developed a model system in which target antigen, bacterial host, and corresponding T cell specificity are defined. OVA-specific T cells from DO11.RAG-2-/- TCR transgenic mice were transferred into RAG-2-/- recipients whose intestinal tracts were colonized with OVA-expressing or control Escherichia coli. Transfer of antigen-naive DO11.RAG-2-/- T cells into recipients colonized with OVA-E. coli resulted in enhanced intestinal recruitment and cell cycling of OVA-specific T cells; however, there was no development of disease. In contrast, transfer of polarized T helper (Th) 1 and Th2 populations resulted in severe wasting and colitis in recipients colonized with OVA-expressing but not control E. coli. The histopathologic features of disease induced by Th1 and Th2 transfers were distinct, but disease severity was comparable. Induction of disease by both Th1 and Th2 transfers was dependent on bacterially associated OVA. These results establish that a single bacterially associated antigen can drive the progression of colitis mediated by both Th1 and Th2 cells and provide a new model for understanding the immunoregulatory interactions between T cells responsive to gut floral antigens. 10.1084/jem.2001889 IS - 1 JF - J. Exp. Med. EP - 84 TI - T Helper 1 and T Helper 2 Cells Are Pathogenic in an Antigen-specific Model of Colitis VL - 195 SP - 71 KW - ecoli KW - ibd AU - Iqbal, Nuzhat AU - Oliver, James AU - Wagner, Frederic AU - Lazenby, Audrey AU - Elson, Charles AU - Weaver, Casey PY - 2002/01/07/ UR - http://dx.doi.org/10.1084/jem.2001889 ER -