CHEMOTHERAPY AND ANTIBIOTIC-RESISTANCE TRANSFER BETWEEN ENTEROBACTERIA IN THE HUMAN GASTRO-INTESTINAL TRACT Export

@article{citeulike:5150020, abstract = {The transfer of R factors in the human gastro-intestinal tract and the conditions under which it occurs was studied in healthy persons, who ingested derivatives of Escherichia coli containing chromosomal and R-factor-mediated resistance determinants that had been prepared from strains isolated from their own faeces. The experiments were designed to obtain presumptive evidence of survival and transfer of R factors by studying resistance-marker patterns of organisms isolated from the faeces. To prove conclusively that R-factor transfer had occurred and that the observed marker patterns were not fortuitous, studies of resistance-marker patterns in one of these subjects were supplemented by further detailed investigations including serological and molecular studies, which will be described in the succeeding paper. An incidental finding was a significant positive correlation between the mean number of antibiotic-resistance determinants in resistant organisms and the age of an individual, although these studies were limited to a small and highly selected group of subjects. No evidence of R-factor transfer was obtained in the absence of chemotherapy in four subjects, even though the plasmids concerned could be freely transferred in vitro from the potential donors to a wide range of faecal organisms, including the genetically marked ingested potential recipients. However, treatment of three subjects with a five-day course of a relevant antibiotic after ingestion of R factor bearing organisms led to the appearance in the faeces of large numbers of resistant coliform bacilli with antibiotic-resistance patterns consistent with transfer of the R factor either to an ingested potential recipient organism (one subject) or to endogenous strains of E. coli (two subjects). Chemotherapy greatly prolonged the persistence of ingested R factors in the faecal flora long after the antibiotics were withdrawn and caused the number of R-factor bearing organisms in the faeces to increase. No evidence was obtained in limited studies of fragmentation or loss of R factors from ingested E. coli. 10.1099/00222615-6-4-461}, author = {Anderson, J. D. and Gillespie, W. A. and Richmond, M. H.}, citeulike-article-id = {5150020}, citeulike-linkout-0 = {http://dx.doi.org/10.1099/00222615-6-4-461}, citeulike-linkout-1 = {http://jmm.sgmjournals.org/cgi/content/abstract/6/4/461}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/4587394}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=4587394}, comment = {In the paper humans volunteers are fed commensal bacteria isolated from their own feces}, day = {1}, doi = {10.1099/00222615-6-4-461}, journal = {J Med Microbiol}, keywords = {ecoli}, month = {November}, number = {4}, pages = {461--473}, posted-at = {2009-07-14 18:50:23}, priority = {2}, title = {CHEMOTHERAPY AND ANTIBIOTIC-RESISTANCE TRANSFER BETWEEN ENTEROBACTERIA IN THE HUMAN GASTRO-INTESTINAL TRACT}, url = {http://dx.doi.org/10.1099/00222615-6-4-461}, volume = {6}, year = {1973} }

TY - JOUR ID - citeulike:5150020 L3 - citeulike-article-id:5150020 N2 - The transfer of R factors in the human gastro-intestinal tract and the conditions under which it occurs was studied in healthy persons, who ingested derivatives of Escherichia coli containing chromosomal and R-factor-mediated resistance determinants that had been prepared from strains isolated from their own faeces. The experiments were designed to obtain presumptive evidence of survival and transfer of R factors by studying resistance-marker patterns of organisms isolated from the faeces. To prove conclusively that R-factor transfer had occurred and that the observed marker patterns were not fortuitous, studies of resistance-marker patterns in one of these subjects were supplemented by further detailed investigations including serological and molecular studies, which will be described in the succeeding paper. An incidental finding was a significant positive correlation between the mean number of antibiotic-resistance determinants in resistant organisms and the age of an individual, although these studies were limited to a small and highly selected group of subjects. No evidence of R-factor transfer was obtained in the absence of chemotherapy in four subjects, even though the plasmids concerned could be freely transferred in vitro from the potential donors to a wide range of faecal organisms, including the genetically marked ingested potential recipients. However, treatment of three subjects with a five-day course of a relevant antibiotic after ingestion of R factor bearing organisms led to the appearance in the faeces of large numbers of resistant coliform bacilli with antibiotic-resistance patterns consistent with transfer of the R factor either to an ingested potential recipient organism (one subject) or to endogenous strains of E. coli (two subjects). Chemotherapy greatly prolonged the persistence of ingested R factors in the faecal flora long after the antibiotics were withdrawn and caused the number of R-factor bearing organisms in the faeces to increase. No evidence was obtained in limited studies of fragmentation or loss of R factors from ingested E. coli. 10.1099/00222615-6-4-461 IS - 4 JF - J Med Microbiol EP - 473 TI - CHEMOTHERAPY AND ANTIBIOTIC-RESISTANCE TRANSFER BETWEEN ENTEROBACTERIA IN THE HUMAN GASTRO-INTESTINAL TRACT VL - 6 SP - 461 KW - ecoli N1 - In the paper humans volunteers are fed commensal bacteria isolated from their own feces AU - Anderson, JD AU - Gillespie, WA AU - Richmond, MH PY - 1973/11/01/ UR - http://dx.doi.org/10.1099/00222615-6-4-461 ER -