Cryptopatches and isolated lymphoid follicles: dynamic lymphoid tissues dispensable for the generation of intraepithelial lymphocytes Export

@article{citeulike:5295674, abstract = {In comparison to secondary lymphoid organs, gut-associated lymphoid tissues such as isolated lymphoid follicles (ILF) and cryptopatches (CP) have been less intensively studied. To gain a better insight into processes regulating organization and function of these structures, which are believed to participate in immune responses and extrathymic T cell development, we characterized the lymphoid structures of the murine small intestine in more detail. The size and cellular composition of small intestinal lymphoid aggregations were analyzed in C57BL/6 and BALB/c wild-type and lymphotoxin (LT)-deficient mice, by flow cytometry, histology and automated multi-color immunofluorescence microscopy evaluating large coherent areas of the intestine. These evaluations demonstrate that aggregated lymphoid structures in the small intestine vary in size and cellular composition, with a majority of structures not matching the current definitions of CP or ILF. Accordingly, significant variations depending on species, age and mouse strain were observed. Furthermore, small bowel transplantation revealed a rapid exchange of B but not T cells between host and grafted tissue. Moreover, LT-deficient animals lack any intestinal lymphoid aggregations yet possess the complete panel of intraepithelial lymphocytes (IEL). In summary, our observations disclose intestinal lymphoid aggregations as dynamic structures with a great deal of inborn plasticity and demonstrate their dispensability for the generation of IEL.}, address = {Institute of Immunology, Hannover Medical School, Hannover, Germany; Department of Visceral and Transplantation Surgery, Hannover Medical School, Hannover, Germany; Comparative Genomics Centre, James Cook University, Townsville, Australia; Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany}, author = {Pabst, Oliver and Herbrand, Heike and Worbs, Tim and Friedrichsen, Michaela and Yan, Sheng and Matthias w and K\"{o}rner, Heiner and Bernhardt, G\"{u}nter and Pabst, Reinhard and F\"{o}rster, Reinhold}, citeulike-article-id = {5295674}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/eji.200425432}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/109803186/ABSTRACT}, comment = {Cryptopatches (CP), numerous small and randomly distributed clusters of lymphoid cells in the basal lamina propria of the murine intestine, are composed of predominantly lineage-negative (CD3-CD4-CD8-B220-) cells expressing the stem cell factor c-kit ARE THEY PRESENT IN LARGE AND SMALL? ---=note-separator=--- 100-150 ILF, very much like PP, colonize the small intestine along the antimesenteric wall ---=note-separator=--- LT- and LT-deficient animals do not develop any aggregated lymphoid structures in the intestine yet possess all intestine-specific IEL subpopulations, demonstrating that intestinal lymphoid aggregations are dispensable for IEL formation. ---=note-separator=--- LTalpha mutants might relate to the overall susceptibility of these animals to autoimmune diseases, including inflammatory bowel disease [15] Spahn TW Am J Pathol 2002 ---=note-separator=--- in C57BL/6 mice 4-6 months of age, these structures are about twice as large with respect to the diameter as in 6-8-week-old animals}, doi = {10.1002/eji.200425432}, issn = {1521-4141}, journal = {European Journal of Immunology}, keywords = {ilfs, lti-cells}, number = {1}, pages = {98--107}, posted-at = {2009-07-28 22:37:24}, priority = {2}, title = {Cryptopatches and isolated lymphoid follicles: dynamic lymphoid tissues dispensable for the generation of intraepithelial lymphocytes}, url = {http://dx.doi.org/10.1002/eji.200425432}, volume = {35}, year = {2005} }

TY - JOUR ID - citeulike:5295674 L3 - citeulike-article-id:5295674 N2 - In comparison to secondary lymphoid organs, gut-associated lymphoid tissues such as isolated lymphoid follicles (ILF) and cryptopatches (CP) have been less intensively studied. To gain a better insight into processes regulating organization and function of these structures, which are believed to participate in immune responses and extrathymic T cell development, we characterized the lymphoid structures of the murine small intestine in more detail. The size and cellular composition of small intestinal lymphoid aggregations were analyzed in C57BL/6 and BALB/c wild-type and lymphotoxin (LT)-deficient mice, by flow cytometry, histology and automated multi-color immunofluorescence microscopy evaluating large coherent areas of the intestine. These evaluations demonstrate that aggregated lymphoid structures in the small intestine vary in size and cellular composition, with a majority of structures not matching the current definitions of CP or ILF. Accordingly, significant variations depending on species, age and mouse strain were observed. Furthermore, small bowel transplantation revealed a rapid exchange of B but not T cells between host and grafted tissue. Moreover, LT-deficient animals lack any intestinal lymphoid aggregations yet possess the complete panel of intraepithelial lymphocytes (IEL). In summary, our observations disclose intestinal lymphoid aggregations as dynamic structures with a great deal of inborn plasticity and demonstrate their dispensability for the generation of IEL. IS - 1 JF - European Journal of Immunology SN - 1521-4141 AD - Institute of Immunology, Hannover Medical School, Hannover, Germany; Department of Visceral and Transplantation Surgery, Hannover Medical School, Hannover, Germany; Comparative Genomics Centre, James Cook University, Townsville, Australia; Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany EP - 107 TI - Cryptopatches and isolated lymphoid follicles: dynamic lymphoid tissues dispensable for the generation of intraepithelial lymphocytes VL - 35 SP - 98 KW - ilfs KW - lti-cells N1 - Cryptopatches (CP), numerous small and randomly distributed clusters of lymphoid cells in the basal lamina propria of the murine intestine, are composed of predominantly lineage-negative (CD3-CD4-CD8-B220-) cells expressing the stem cell factor c-kit ARE THEY PRESENT IN LARGE AND SMALL? N1 - 100-150 ILF, very much like PP, colonize the small intestine along the antimesenteric wall N1 - LT- and LT-deficient animals do not develop any aggregated lymphoid structures in the intestine yet possess all intestine-specific IEL subpopulations, demonstrating that intestinal lymphoid aggregations are dispensable for IEL formation. N1 - LTalpha mutants might relate to the overall susceptibility of these animals to autoimmune diseases, including inflammatory bowel disease [15] Spahn TW Am J Pathol 2002 N1 - in C57BL/6 mice 4-6 months of age, these structures are about twice as large with respect to the diameter as in 6-8-week-old animals AU - Pabst, Oliver AU - Herbrand, Heike AU - Worbs, Tim AU - Friedrichsen, Michaela AU - Yan, Sheng AU - Matthias w AU - Körner, Heiner AU - Bernhardt, Günter AU - Pabst, Reinhard AU - Förster, Reinhold PY - 2005/// UR - http://dx.doi.org/10.1002/eji.200425432 ER -