Role of Nod1 in mucosal dendritic cells during Spi-1 independent Salmonella infection. Export

@article{citeulike:5636724, abstract = {Recent advances in immunology have highlighted the critical function of pattern-recognition molecules (PRM) in generating the innate immune response to effectively target pathogens. Nod1 and Nod2 are intracellular PRMs that detect peptidoglycan motifs from the cell wall of bacteria once they gain access to the cytosol. Salmonella enterica serovar Typhimurium (S. Typhimurium) is an enteric intracellular pathogen that causes a severe disease in the mouse model. This pathogen resides within vacuoles inside the cell but the question of whether cytosolic PRMs like Nod1 and Nod2 could impact on the course of S. Typhimurium infection in vivo has not been addressed. Here, we show that deficiency in the PRM Nod1, but not Nod2, resulted in increased susceptibility towards a mutant strain of S. Typhimurium that targets directly lamina propria dendritic cells (DCs) for its entry into the host. Using this bacterium and bone-marrow chimeras, we uncovered a surprising role for Nod1 in myeloid cells controlling bacterial infection at the level of the intestinal lamina propria. Indeed, DCs deficient for Nod1 exhibited impaired clearance of the bacteria, both in vitro and in vivo, leading to increased organ colonization and decreased host survival after oral infection. Taken together, these findings demonstrate a key role for Nod1 in the host response to an enteric bacterial pathogen through the modulation of intestinal lamina propria DCs.}, author = {Le Bourhis, Lionel and Magalhaes, Joao Gamelas G. and Selvanantham, Thirumahal and Travassos, Leonardo H. and Geddes, Kaoru and Fritz, J\"{o}rg H. and Viala, J\'{e}r\^{o}me and Tedin, Karsten and Girardin, Stephen E. and Philpott, Dana J.}, citeulike-article-id = {5636724}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/IAI.00519-09}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19620349}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19620349}, day = {20}, doi = {10.1128/IAI.00519-09}, issn = {1098-5522}, journal = {Infection and immunity}, keywords = {intestinal-dcs, nod1}, month = {July}, posted-at = {2009-08-25 00:50:32}, priority = {2}, title = {Role of Nod1 in mucosal dendritic cells during Spi-1 independent Salmonella infection.}, url = {http://dx.doi.org/10.1128/IAI.00519-09}, year = {2009} }

TY - JOUR ID - citeulike:5636724 L3 - citeulike-article-id:5636724 N2 - Recent advances in immunology have highlighted the critical function of pattern-recognition molecules (PRM) in generating the innate immune response to effectively target pathogens. Nod1 and Nod2 are intracellular PRMs that detect peptidoglycan motifs from the cell wall of bacteria once they gain access to the cytosol. Salmonella enterica serovar Typhimurium (S. Typhimurium) is an enteric intracellular pathogen that causes a severe disease in the mouse model. This pathogen resides within vacuoles inside the cell but the question of whether cytosolic PRMs like Nod1 and Nod2 could impact on the course of S. Typhimurium infection in vivo has not been addressed. Here, we show that deficiency in the PRM Nod1, but not Nod2, resulted in increased susceptibility towards a mutant strain of S. Typhimurium that targets directly lamina propria dendritic cells (DCs) for its entry into the host. Using this bacterium and bone-marrow chimeras, we uncovered a surprising role for Nod1 in myeloid cells controlling bacterial infection at the level of the intestinal lamina propria. Indeed, DCs deficient for Nod1 exhibited impaired clearance of the bacteria, both in vitro and in vivo, leading to increased organ colonization and decreased host survival after oral infection. Taken together, these findings demonstrate a key role for Nod1 in the host response to an enteric bacterial pathogen through the modulation of intestinal lamina propria DCs. JF - Infection and immunity SN - 1098-5522 TI - Role of Nod1 in mucosal dendritic cells during Spi-1 independent Salmonella infection. KW - intestinal-dcs KW - nod1 AU - Le Bourhis, Lionel AU - Magalhaes, Joao Gamelas AU - Selvanantham, Thirumahal AU - Travassos, Leonardo AU - Geddes, Kaoru AU - Fritz, Jörg AU - Viala, Jérôme AU - Tedin, Karsten AU - Girardin, Stephen AU - Philpott, Dana PY - 2009/07/20/ UR - http://dx.doi.org/10.1128/IAI.00519-09 ER -