Lymph Node Stromal Cells Support Dendritic Cell-Induced Gut-Homing of T Cells Export

@article{citeulike:6090385, abstract = {T cells are imprinted to express tissue-specific homing receptors upon activation in tissue-draining lymph nodes, resulting in their migration to the site of Ag entry. Expression of gut-homing molecules {alpha}4{beta}7 and CCR9 is induced by retinoic acid, a vitamin A metabolite produced by retinal dehydrogenases, which are specifically expressed in dendritic cells as well as stromal cells in mucosa-draining lymph nodes. In this study, we demonstrate that mesenteric lymph node stromal cell-derived retinoic acid can directly induce the expression of gut-homing molecules on proliferating T cells, a process strongly enhanced by bone marrow-derived dendritic cells in vitro. Therefore, cooperation of sessile lymph node stromal cells with mobile dendritic cells warrants the imprinting of tissue specific homing receptors on activated T cells. 10.4049/jimmunol.0900311}, author = {Molenaar, Rosalie and Greuter, Mascha and van der Marel, Arnold P. J. and Roozendaal, Ramon and Martin, Stefan F. and Edele, Fanny and Huehn, Jochen and Forster, Reinhold and O'Toole, Tom and Jansen, Wendy and Eestermans, Inge L. and Kraal, Georg and Mebius, Reina E.}, citeulike-article-id = {6090385}, citeulike-linkout-0 = {http://dx.doi.org/10.4049/jimmunol.0900311}, citeulike-linkout-1 = {http://www.jimmunol.org/content/183/10/6395.abstract}, citeulike-linkout-2 = {http://www.jimmunol.org/content/183/10/6395.full.pdf}, citeulike-linkout-3 = {http://www.jimmunol.org/cgi/content/abstract/183/10/6395}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/19841174}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=19841174}, comment = {To elucidate the role of the LN microenvironment in providing signals for T cell tropism, either gut or skin draining LNs were transplanted into a peripheral site, the popliteal fossa. ---=note-separator=--- MLNs stroma expresses RALDH2 (and also RALDH1 and RALDH3) but culturing MLN stroma + BM-DCs did not upregulate RALDH2 expression in the DCs (BM cells were differentiated into DCs with GM-CSF). ---=note-separator=--- MLNs stroma expresses higher levels of RALDH1,2 and 3 than stroma from pLNs ---=note-separator=--- References 32-34 show intimate interaction between LN stromal cells and DCs ---=note-separator=--- Within the MLNs, CD103+ DCs are able to induce the expression of both alpha4beta7 and CCR9, while CD103- DCs are only capable of inducing alpha4beta7 (35, 36). Because also alpha4beta7+ CCR9- cells were shown to migrate to the intestines, both DC subsets can induce gut-homing tropism (9). ---=note-separator=--- CD103+ DCs migrate from the LP to the MLNs CD103- DCs enter MLNs via blood refs 1 35-38}, day = {15}, doi = {10.4049/jimmunol.0900311}, journal = {J Immunol}, keywords = {ilfs, lti-cells}, month = {November}, number = {10}, pages = {6395--6402}, posted-at = {2009-11-09 19:50:16}, priority = {2}, title = {Lymph Node Stromal Cells Support Dendritic Cell-Induced Gut-Homing of T Cells}, url = {http://dx.doi.org/10.4049/jimmunol.0900311}, volume = {183}, year = {2009} }

TY - JOUR ID - citeulike:6090385 L3 - citeulike-article-id:6090385 N2 - T cells are imprinted to express tissue-specific homing receptors upon activation in tissue-draining lymph nodes, resulting in their migration to the site of Ag entry. Expression of gut-homing molecules {alpha}4{beta}7 and CCR9 is induced by retinoic acid, a vitamin A metabolite produced by retinal dehydrogenases, which are specifically expressed in dendritic cells as well as stromal cells in mucosa-draining lymph nodes. In this study, we demonstrate that mesenteric lymph node stromal cell-derived retinoic acid can directly induce the expression of gut-homing molecules on proliferating T cells, a process strongly enhanced by bone marrow-derived dendritic cells in vitro. Therefore, cooperation of sessile lymph node stromal cells with mobile dendritic cells warrants the imprinting of tissue specific homing receptors on activated T cells. 10.4049/jimmunol.0900311 IS - 10 JF - J Immunol EP - 6402 TI - Lymph Node Stromal Cells Support Dendritic Cell-Induced Gut-Homing of T Cells VL - 183 SP - 6395 KW - ilfs KW - lti-cells N1 - To elucidate the role of the LN microenvironment in providing signals for T cell tropism, either gut or skin draining LNs were transplanted into a peripheral site, the popliteal fossa. N1 - MLNs stroma expresses RALDH2 (and also RALDH1 and RALDH3) but culturing MLN stroma + BM-DCs did not upregulate RALDH2 expression in the DCs (BM cells were differentiated into DCs with GM-CSF). N1 - MLNs stroma expresses higher levels of RALDH1,2 and 3 than stroma from pLNs N1 - References 32-34 show intimate interaction between LN stromal cells and DCs N1 - Within the MLNs, CD103+ DCs are able to induce the expression of both alpha4beta7 and CCR9, while CD103- DCs are only capable of inducing alpha4beta7 (35, 36). Because also alpha4beta7+ CCR9- cells were shown to migrate to the intestines, both DC subsets can induce gut-homing tropism (9). N1 - CD103+ DCs migrate from the LP to the MLNs CD103- DCs enter MLNs via blood refs 1 35-38 AU - Molenaar, Rosalie AU - Greuter, Mascha AU - van der Marel, Arnold AU - Roozendaal, Ramon AU - Martin, Stefan AU - Edele, Fanny AU - Huehn, Jochen AU - Forster, Reinhold AU - O'Toole, Tom AU - Jansen, Wendy AU - Eestermans, Inge AU - Kraal, Georg AU - Mebius, Reina PY - 2009/11/15/ UR - http://dx.doi.org/10.4049/jimmunol.0900311 ER -