Lung Pathology in Fatal Novel Human Influenza A (H1N1) Infection. Export

@article{citeulike:6072547, abstract = {RATIONALE: There are no reports of the systemic human pathology of the novel swine H1N1 influenza (S-OIV) infection. OBJECTIVES: The autopsy findings of 21 Brazilian patients with confirmed S-OIV infection are presented. These patients died in the winter of the southern hemisphere 2009 pandemic, with acute respiratory failure. METHODS: Lung tissue was submitted to virologic and bacteriologic analysis with rRT-PCR and electron microscopy. Expression of Toll-like receptor (TLR)-3, interferon-gamma, TNF-alpha, CD8+T cells and granzyme B+ cells in the lungs was investigated by immunohistochemistry. RESULTS: Patients were aged from one to 68 years (72\% between 30 and 59 years), and 12 were male. Sixteen patients had preexisting medical conditions. Diffuse alveolar damage (DAD) was present in 20 individuals. In six patients, DAD was associated with necrotizing bronchiolitis and in 5 with extensive hemorrhage. There was also a cytopathic effect in the bronchial and alveolar epithelial cells, as well as necrosis, epithelial hyperplasia and squamous metaplasia of the large airways. There was marked expression of TLR-3 and IFN-gamma and a large number of CD8+ T cells and granzyme B+ cells within the lung tissue. Changes in other organs were mainly secondary to multiple organ failure. CONCLUSIONS: Autopsies have shown that the main pathological changes associated with S-OIV infection are localized to the lungs, where three distinct histological patterns can be identified. We also show evidence of ongoing pulmonary aberrant immune response. Our results reinforce the utility of autopsy in increasing the understanding of the novel human influenza A (H1N1) infection.}, author = {Mauad, Thais and Hajjar, Ludhmila Abrahao A. and Callegari, Giovanna de Sanctis D. and da Silva, Luiz Fernando Ferraz F. and Schout, Denise and Galas, Filomena Regina Barbosa Gomes R. and Alves, Venancio Avancini Ferreira A. and Malheiros, Denise Maria Avancini Costa M. and Auler Jr, Jose Otavio Costa O. and Ferreira, Aurea Favero F. and Borsato, Marcela Regina Longo R. and Bezerra, Stephania Martins M. and Gutierrez, Paulo Sampaio S. and Caldini, Elia Tamaso Espin Garcia T. and Pasqualucci, Carlos Augusto A. and Dolhnikoff, Marisa and Saldiva, Paulo Hilario Nascimento H.}, citeulike-article-id = {6072547}, citeulike-linkout-0 = {http://dx.doi.org/10.1164/rccm.200909-1420OC}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19875682}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19875682}, day = {29}, doi = {10.1164/rccm.200909-1420OC}, issn = {1535-4970}, journal = {American journal of respiratory and critical care medicine}, keywords = {a-h1n1, lung\_pathology}, month = {October}, posted-at = {2009-11-05 07:12:46}, priority = {2}, title = {Lung Pathology in Fatal Novel Human Influenza A (H1N1) Infection.}, url = {http://dx.doi.org/10.1164/rccm.200909-1420OC}, year = {2009} }

TY - JOUR ID - citeulike:6072547 L3 - citeulike-article-id:6072547 N2 - RATIONALE: There are no reports of the systemic human pathology of the novel swine H1N1 influenza (S-OIV) infection. OBJECTIVES: The autopsy findings of 21 Brazilian patients with confirmed S-OIV infection are presented. These patients died in the winter of the southern hemisphere 2009 pandemic, with acute respiratory failure. METHODS: Lung tissue was submitted to virologic and bacteriologic analysis with rRT-PCR and electron microscopy. Expression of Toll-like receptor (TLR)-3, interferon-gamma, TNF-alpha, CD8+T cells and granzyme B+ cells in the lungs was investigated by immunohistochemistry. RESULTS: Patients were aged from one to 68 years (72% between 30 and 59 years), and 12 were male. Sixteen patients had preexisting medical conditions. Diffuse alveolar damage (DAD) was present in 20 individuals. In six patients, DAD was associated with necrotizing bronchiolitis and in 5 with extensive hemorrhage. There was also a cytopathic effect in the bronchial and alveolar epithelial cells, as well as necrosis, epithelial hyperplasia and squamous metaplasia of the large airways. There was marked expression of TLR-3 and IFN-gamma and a large number of CD8+ T cells and granzyme B+ cells within the lung tissue. Changes in other organs were mainly secondary to multiple organ failure. CONCLUSIONS: Autopsies have shown that the main pathological changes associated with S-OIV infection are localized to the lungs, where three distinct histological patterns can be identified. We also show evidence of ongoing pulmonary aberrant immune response. Our results reinforce the utility of autopsy in increasing the understanding of the novel human influenza A (H1N1) infection. JF - American journal of respiratory and critical care medicine SN - 1535-4970 TI - Lung Pathology in Fatal Novel Human Influenza A (H1N1) Infection. KW - a-h1n1 KW - lung_pathology AU - Mauad, Thais AU - Hajjar, Ludhmila Abrahao AU - Callegari, Giovanna de Sanctis AU - da Silva, Luiz Fernando Ferraz AU - Schout, Denise AU - Galas, Filomena Regina Barbosa Gomes AU - Alves, Venancio Avancini Ferreira AU - Malheiros, Denise Maria Avancini Costa AU - Auler Jr, Jose Otavio Costa AU - Ferreira, Aurea Favero AU - Borsato, Marcela Regina Longo AU - Bezerra, Stephania Martins AU - Gutierrez, Paulo Sampaio AU - Caldini, Elia Tamaso Espin Garcia AU - Pasqualucci, Carlos Augusto AU - Dolhnikoff, Marisa AU - Saldiva, Paulo Hilario Nascimento PY - 2009/10/29/ UR - http://dx.doi.org/10.1164/rccm.200909-1420OC ER -