Identification of mammal species using species-specific DNA pyrosequencing. Export

@article{citeulike:1185752, abstract = {In forensic casework it is highly relevant to be able to deduce the species origin of an unknown biological sample. For such a purpose we have designed and developed an assay for species identification based on DNA sequencing of two short mitochondrial DNA amplicons. In short, partial 12S rRNA and partial 16S rRNA fragments ( approximately 100bp) are amplified by PCR followed by direct sequencing using pyrosequencing technique. Due to properties of the chosen targets, the same PCR conditions and primers were used irrespective of the true species of an unknown sample. A total of 28 different mammals present in the European fauna were sequenced both for the partial 12S rRNA and the partial 16S rRNA sequences for accuracy verification. Together the two sequences showed to have a high divergence factor, discriminating almost all mammals. Furthermore, the human reference nucleotide sequences were always at least nine nucleotides different compared to the other sequenced species both at the partial 12S rRNA and the partial 16S rRNA sequences.}, address = {The National Board of Forensic Medicine, Department of Forensic Genetics and Forensic Toxicology, Artillerigatan 12, SE-581 33 Link\"{o}ping, Sweden.}, author = {Karlsson, Andreas O O. and Holmlund, Gunilla}, citeulike-article-id = {1185752}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.forsciint.2007.01.019}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17331687}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17331687}, day = {27}, doi = {10.1016/j.forsciint.2007.01.019}, issn = {0379-0738}, journal = {Forensic Sci Int}, keywords = {12s, 16s, mt, phylogeny, pyrosequencing, rrna, species}, month = {February}, posted-at = {2007-03-25 03:05:26}, priority = {4}, title = {Identification of mammal species using species-specific DNA pyrosequencing.}, url = {http://dx.doi.org/10.1016/j.forsciint.2007.01.019}, year = {2007} }

TY - JOUR ID - citeulike:1185752 L3 - citeulike-article-id:1185752 N2 - In forensic casework it is highly relevant to be able to deduce the species origin of an unknown biological sample. For such a purpose we have designed and developed an assay for species identification based on DNA sequencing of two short mitochondrial DNA amplicons. In short, partial 12S rRNA and partial 16S rRNA fragments ( approximately 100bp) are amplified by PCR followed by direct sequencing using pyrosequencing technique. Due to properties of the chosen targets, the same PCR conditions and primers were used irrespective of the true species of an unknown sample. A total of 28 different mammals present in the European fauna were sequenced both for the partial 12S rRNA and the partial 16S rRNA sequences for accuracy verification. Together the two sequences showed to have a high divergence factor, discriminating almost all mammals. Furthermore, the human reference nucleotide sequences were always at least nine nucleotides different compared to the other sequenced species both at the partial 12S rRNA and the partial 16S rRNA sequences. JF - Forensic Sci Int SN - 0379-0738 AD - The National Board of Forensic Medicine, Department of Forensic Genetics and Forensic Toxicology, Artillerigatan 12, SE-581 33 Linköping, Sweden. TI - Identification of mammal species using species-specific DNA pyrosequencing. KW - 12s KW - 16s KW - mt KW - phylogeny KW - pyrosequencing KW - rrna KW - species AU - Karlsson, Andreas O AU - Holmlund, Gunilla PY - 2007/02/27/ UR - http://dx.doi.org/10.1016/j.forsciint.2007.01.019 ER -