Factor V Leiden in an Urartian, Dating Back to 1000 BC. Export

@article{citeulike:5978692, abstract = {Factor V Leiden (FVL) is the most common monogenic disorder that causes activated protein C (APC) resistance, creating hypercoagulation. The mutation shows an uneven geographic distribution, significantly high in European populations. The mutation is believed to have originated approximately 20 000 years ago probably from a geographic region close to Anatolia. This fact makes it noteworthy to search for the mutation in ancient populations that once lived in this area. One of these civilizations, Urartu was centered around Van Lake in Eastern Turkey. The archeological remains from the excavations of the region are dated back to 1000 <scp>BC</scp>. Teeth, taken from the excavations of Van Yoncatepe fortress, were taken into DNA analysis considering all the precautions for ancient DNA analysis. Multiplex STR (Short Tandem Repeats) analysis were performed both to determine the gender of the samples and to conclude that the samples are preserved from modern DNA contamination. After getting an 80\% amplification success for amelogenin, a melting curve analysis using lightcycler was performed to determine the FVL genotype of each sample. Of the 60 samples, 1 gave a positive amplification result for FV gene and was found to be heterozygous. To date, the age of this mutation was estimated based on statistical calculations using haplotype frequencies; here for the first time, we report FVL in an ancient population of 3000 years.}, author = {Dogan Alakoc, Yesim and Aka, Sema and Egin, Yonca and Akar, Nejat}, citeulike-article-id = {5978692}, citeulike-linkout-0 = {http://dx.doi.org/10.1177/1076029609338045}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19617246}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19617246}, day = {17}, doi = {10.1177/1076029609338045}, journal = {Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis}, keywords = {ancient, autosomal, factor\_v\_leiden, near\_east, urartu}, month = {July}, posted-at = {2009-10-21 05:56:58}, priority = {5}, title = {Factor V Leiden in an Urartian, Dating Back to 1000 BC.}, url = {http://dx.doi.org/10.1177/1076029609338045}, year = {2009} }

TY - JOUR ID - citeulike:5978692 L3 - citeulike-article-id:5978692 N2 - Factor V Leiden (FVL) is the most common monogenic disorder that causes activated protein C (APC) resistance, creating hypercoagulation. The mutation shows an uneven geographic distribution, significantly high in European populations. The mutation is believed to have originated approximately 20 000 years ago probably from a geographic region close to Anatolia. This fact makes it noteworthy to search for the mutation in ancient populations that once lived in this area. One of these civilizations, Urartu was centered around Van Lake in Eastern Turkey. The archeological remains from the excavations of the region are dated back to 1000 <scp>BC</scp>. Teeth, taken from the excavations of Van Yoncatepe fortress, were taken into DNA analysis considering all the precautions for ancient DNA analysis. Multiplex STR (Short Tandem Repeats) analysis were performed both to determine the gender of the samples and to conclude that the samples are preserved from modern DNA contamination. After getting an 80% amplification success for amelogenin, a melting curve analysis using lightcycler was performed to determine the FVL genotype of each sample. Of the 60 samples, 1 gave a positive amplification result for FV gene and was found to be heterozygous. To date, the age of this mutation was estimated based on statistical calculations using haplotype frequencies; here for the first time, we report FVL in an ancient population of 3000 years. JF - Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis TI - Factor V Leiden in an Urartian, Dating Back to 1000 BC. KW - ancient KW - autosomal KW - factor_v_leiden KW - near_east KW - urartu AU - Dogan Alakoc, Yesim AU - Aka, Sema AU - Egin, Yonca AU - Akar, Nejat PY - 2009/07/17/ UR - http://dx.doi.org/10.1177/1076029609338045 ER -