A germin-like protein of wheat leaf apoplast inhibits serine proteases Export

@article{Segarra2003, abstract = {A protein resistant to heat and proteolysis that inhibits serine proteases was isolated from wheat leaf apoplasts. Based on trypsin inhibition, its more active form was a 66-69 kDa oligomer. It was dissociated in an 18-21 kDa monomer having an amino terminal sequence identical to the Box A of germins and germin-like proteins. Like these proteins, it was glycosylated and showed manganese superoxide dismutase activity. The monomer displayed three forms when examined by 2D western blot: two of 19 kDa, pI 5.8 and 6.2; and one of 21 kDa, pI 5.8. It was found that the protein controls serine protease activity in the apoplast of plants challenged with the fungus Septoria tritici. 10.1093/jxb/erg139}, author = {Segarra, Carmen I. and Casalongue, Claudia A. and Pinedo, Marcela L. and Ronchi, Virginia P. and Conde, Ruben D.}, citeulike-article-id = {3736728}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/jxb/erg139}, citeulike-linkout-1 = {http://jxb.oxfordjournals.org/cgi/content/abstract/54/386/1335}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/12709479}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=12709479}, day = {1}, doi = {10.1093/jxb/erg139}, journal = {J. Exp. Bot.}, keywords = {germin-like, inhibitor, serine\_protease, triticum}, month = {May}, number = {386}, pages = {1335--1341}, posted-at = {2008-12-02 18:56:26}, priority = {2}, title = {A germin-like protein of wheat leaf apoplast inhibits serine proteases}, url = {http://dx.doi.org/10.1093/jxb/erg139}, volume = {54}, year = {2003} }

TY - JOUR ID - Segarra2003 L3 - citeulike-article-id:3736728 N2 - A protein resistant to heat and proteolysis that inhibits serine proteases was isolated from wheat leaf apoplasts. Based on trypsin inhibition, its more active form was a 66-69 kDa oligomer. It was dissociated in an 18-21 kDa monomer having an amino terminal sequence identical to the Box A of germins and germin-like proteins. Like these proteins, it was glycosylated and showed manganese superoxide dismutase activity. The monomer displayed three forms when examined by 2D western blot: two of 19 kDa, pI 5.8 and 6.2; and one of 21 kDa, pI 5.8. It was found that the protein controls serine protease activity in the apoplast of plants challenged with the fungus Septoria tritici. 10.1093/jxb/erg139 IS - 386 JF - J. Exp. Bot. EP - 1341 TI - A germin-like protein of wheat leaf apoplast inhibits serine proteases VL - 54 SP - 1335 KW - germin-like KW - inhibitor KW - serine_protease KW - triticum AU - Segarra, Carmen AU - Casalongue, Claudia AU - Pinedo, Marcela AU - Ronchi, Virginia AU - Conde, Ruben PY - 2003/05/01/ UR - http://dx.doi.org/10.1093/jxb/erg139 ER -