C-Terminal Domain of a Hevein-Like Protein from Wasabia japonica has Potent Antimicrobial Activity Export

@article{Kiba2003, abstract = {An antimicrobial protein, designated WjAMP-1, was purified from leaves of Wasabia japonica L. WjAMP-1 showed antimicrobial activity against both fungi and bacteria. The deduced amino acid sequence of cDNA of WjAMP-1 showed 60\% and 70\% identity with a hevein from Hevea brasiliansis and a hevein-like protein from Arabidopsis thaliana, respectively. However, matured WjAMP-1 lacked the hevein domain and may correspond to the C-terminal domain of hevein. Southern blot analysis showed that one or two copies of the WjAMP-1 gene were presented in the genome of wasabi. Expression of WjAMP-1 was detected in all organs tested, and was especially strong in petioles. Expression of WjAMP-1 was induced by the inoculation with fungal pathogens and treatment with methyl jasmonate. Recombinant WjAMP-1 expressed in Nicotiana benthamiana using potato virus X vector also inhibited not only growth of fungi but also bacteria. These results suggest that WjAMP-1 may be the C-terminal domain of hevein and one of the defense gene in W. japonica. WjAMP-1 gene may be useful genes to generate resistant plants against fungal and bacterial pathogens. 10.1093/pcp/pcg035}, author = {Kiba, A. and Saitoh, H. and Nishihara, M. and Omiya, K. and Yamamura, S.}, citeulike-article-id = {4016281}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/pcp/pcg035}, citeulike-linkout-1 = {http://pcp.oxfordjournals.org/cgi/content/abstract/44/3/296}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/12668776}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=12668776}, day = {15}, doi = {10.1093/pcp/pcg035}, journal = {Plant Cell Physiol.}, keywords = {chitinases, hevein, pr04}, month = {March}, number = {3}, pages = {296--303}, posted-at = {2009-05-25 06:06:45}, priority = {0}, title = {C-Terminal Domain of a Hevein-Like Protein from Wasabia japonica has Potent Antimicrobial Activity}, url = {http://dx.doi.org/10.1093/pcp/pcg035}, volume = {44}, year = {2003} }

TY - JOUR ID - Kiba2003 L3 - citeulike-article-id:4016281 N2 - An antimicrobial protein, designated WjAMP-1, was purified from leaves of Wasabia japonica L. WjAMP-1 showed antimicrobial activity against both fungi and bacteria. The deduced amino acid sequence of cDNA of WjAMP-1 showed 60% and 70% identity with a hevein from Hevea brasiliansis and a hevein-like protein from Arabidopsis thaliana, respectively. However, matured WjAMP-1 lacked the hevein domain and may correspond to the C-terminal domain of hevein. Southern blot analysis showed that one or two copies of the WjAMP-1 gene were presented in the genome of wasabi. Expression of WjAMP-1 was detected in all organs tested, and was especially strong in petioles. Expression of WjAMP-1 was induced by the inoculation with fungal pathogens and treatment with methyl jasmonate. Recombinant WjAMP-1 expressed in Nicotiana benthamiana using potato virus X vector also inhibited not only growth of fungi but also bacteria. These results suggest that WjAMP-1 may be the C-terminal domain of hevein and one of the defense gene in W. japonica. WjAMP-1 gene may be useful genes to generate resistant plants against fungal and bacterial pathogens. 10.1093/pcp/pcg035 IS - 3 JF - Plant Cell Physiol. EP - 303 TI - C-Terminal Domain of a Hevein-Like Protein from Wasabia japonica has Potent Antimicrobial Activity VL - 44 SP - 296 KW - chitinases KW - hevein KW - pr04 AU - Kiba, A AU - Saitoh, H AU - Nishihara, M AU - Omiya, K AU - Yamamura, S PY - 2003/03/15/ UR - http://dx.doi.org/10.1093/pcp/pcg035 ER -