Characterization of a plasmid replicative origin from an extreme thermophile. Export

@article{citeulike:5943953, abstract = {The minimal replicative origin from a 16-kbp natural plasmid of Thermus sp. ATCC 27737 is described. The 1798-bp sequence encodes a single protein (RepA) whose expression is required for replication. This requirement is extended to 200 bp upstream the coding region, which contains promoter-like sequences transcriptionally active in E. coli. Despite the absence of typical iterons, we show that an N-terminal fragment of this protein binds to a DNA fragment internal to its own coding sequence. As this DNA fragment contains an unusually A + T rich sequence surrounded by C + G rich stretches, the possible location of the replicative origin within the region encoding RepA is proposed.}, author = {de Grado, M. and Lasa, I. and Berenguer, J.}, citeulike-article-id = {5943953}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9711839}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9711839}, day = {1}, issn = {0378-1097}, journal = {FEMS microbiology letters}, keywords = {plasmid, thermophile}, month = {August}, number = {1}, pages = {51--57}, posted-at = {2009-10-15 14:26:18}, priority = {2}, title = {Characterization of a plasmid replicative origin from an extreme thermophile.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9711839}, volume = {165}, year = {1998} }

TY - JOUR ID - citeulike:5943953 L3 - citeulike-article-id:5943953 N2 - The minimal replicative origin from a 16-kbp natural plasmid of Thermus sp. ATCC 27737 is described. The 1798-bp sequence encodes a single protein (RepA) whose expression is required for replication. This requirement is extended to 200 bp upstream the coding region, which contains promoter-like sequences transcriptionally active in E. coli. Despite the absence of typical iterons, we show that an N-terminal fragment of this protein binds to a DNA fragment internal to its own coding sequence. As this DNA fragment contains an unusually A + T rich sequence surrounded by C + G rich stretches, the possible location of the replicative origin within the region encoding RepA is proposed. IS - 1 JF - FEMS microbiology letters SN - 0378-1097 EP - 57 TI - Characterization of a plasmid replicative origin from an extreme thermophile. VL - 165 SP - 51 KW - plasmid KW - thermophile AU - de Grado, M AU - Lasa, I AU - Berenguer, J PY - 1998/08/01/ UR - http://view.ncbi.nlm.nih.gov/pubmed/9711839 ER -