Photocurrents Generated by Bacteriorhodopsin Adsorbed on Nano-Black Lipid Membranes Export

@article{horn_pore_05, abstract = {Purple membranes were adsorbed on freestanding lipid bilayers, termed nano-black lipid membranes (nano-BLMs), suspending the pores of porous alumina substrates with average pore diameters of 280 nm. Nano-BLMs were obtained by first coating the upper surface of the highly ordered porous alumina substrates with a thin gold layer followed by chemisorption of 1,2-dipalmitoyl- sn -glycero-3-phosphothioethanol and subsequent addition of a droplet of 1,2-diphytanoyl- sn -glycero-3-phosphocholine and octadecylamine dissolved in n -decane onto the hydrophobic submonolayer. By means of impedance spectroscopy, the quality of the nano-BLMs was verified. The electrical parameters confirm the formation of single lipid bilayers with high membrane resistances covering the porous matrix. Adsorption of purple membranes on the nano-BLMs was followed by recording the photocurrents generated by bacteriorhodopsin upon continuous light illumination. The membrane system exhibits a very high long-term stability with the advantage that not only transient but also stationary currents are recordable. By adding the proton ionophore carbonyl cyanide- m -chlorophenylhydrazone the conductivity of the nano-BLMs increases, resulting in a higher stationary current, which proves that proton conductance occurs across the nano-BLMs.}, author = {Horn, C.}, citeulike-article-id = {5910755}, citeulike-linkout-0 = {http://dx.doi.org/10.1529/biophysj.105.059550}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0006349505727533}, doi = {10.1529/biophysj.105.059550}, issn = {00063495}, journal = {Biophysical Journal}, keywords = {aluminium, bilayer, electrophysiology, lipid, membrane, pore, rhodopsin, slb, spectroscopy}, month = {August}, number = {2}, pages = {1046--1054}, posted-at = {2009-10-08 18:16:40}, priority = {2}, title = {Photocurrents Generated by Bacteriorhodopsin Adsorbed on Nano-Black Lipid Membranes}, url = {http://dx.doi.org/10.1529/biophysj.105.059550}, volume = {89}, year = {2005} }

TY - JOUR ID - horn_pore_05 L3 - citeulike-article-id:5910755 N2 - Purple membranes were adsorbed on freestanding lipid bilayers, termed nano-black lipid membranes (nano-BLMs), suspending the pores of porous alumina substrates with average pore diameters of 280 nm. Nano-BLMs were obtained by first coating the upper surface of the highly ordered porous alumina substrates with a thin gold layer followed by chemisorption of 1,2-dipalmitoyl- sn -glycero-3-phosphothioethanol and subsequent addition of a droplet of 1,2-diphytanoyl- sn -glycero-3-phosphocholine and octadecylamine dissolved in n -decane onto the hydrophobic submonolayer. By means of impedance spectroscopy, the quality of the nano-BLMs was verified. The electrical parameters confirm the formation of single lipid bilayers with high membrane resistances covering the porous matrix. Adsorption of purple membranes on the nano-BLMs was followed by recording the photocurrents generated by bacteriorhodopsin upon continuous light illumination. The membrane system exhibits a very high long-term stability with the advantage that not only transient but also stationary currents are recordable. By adding the proton ionophore carbonyl cyanide- m -chlorophenylhydrazone the conductivity of the nano-BLMs increases, resulting in a higher stationary current, which proves that proton conductance occurs across the nano-BLMs. IS - 2 JF - Biophysical Journal SN - 00063495 EP - 1054 TI - Photocurrents Generated by Bacteriorhodopsin Adsorbed on Nano-Black Lipid Membranes VL - 89 SP - 1046 KW - aluminium KW - bilayer KW - electrophysiology KW - lipid KW - membrane KW - pore KW - rhodopsin KW - slb KW - spectroscopy AU - Horn, C PY - 2005/08// UR - http://dx.doi.org/10.1529/biophysj.105.059550 ER -