Detection of a Biomarker for Alzheimer's Disease from Synthetic and Clinical Samples Using a Nanoscale Optical Biosensor Export

@article{haes_addl_05, abstract = {PMID: 15713105 A nanoscale optical biosensor based on localized surface plasmon resonance (LSPR) spectroscopy has been developed to monitor the interaction between the antigen, amyloid-beta derived diffusible ligands (ADDLs), and specific anti-ADDL antibodies. Using the sandwich assay format, this nanosensor provides quantitative binding information for both antigen and second antibody detection that permits the determination of ADDL concentration and offers the unique analysis of the aggregation mechanisms of this putative Alzheimer's disease pathogen at physiologically relevant monomer concentrations. Monitoring the LSPR-induced shifts from both ADDLs and a second polyclonal anti-ADDL antibody as a function of ADDL concentration reveals two ADDL epitopes that have binding constants to the specific anti-ADDL antibodies of 7.3 1012 M-1 and 9.5 108 M-1. The analysis of human brain extract and cerebrospinal fluid samples from control and Alzheimer's disease patients reveals that the LSPR nanosensor provides new information relevant to the understanding and possible diagnosis of Alzheimer's disease.}, author = {Haes, Amanda J. and Chang, Lei and Klein, William L. and Van Duyne, Richard P.}, citeulike-article-id = {5985388}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/ja044087q}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/ja044087q}, day = {1}, doi = {10.1021/ja044087q}, journal = {Journal of the American Chemical Society}, keywords = {alzheimers, antibody, csf, lithography, lspr, nsl, protein, silver, spectroscopy}, month = {February}, number = {7}, pages = {2264--2271}, posted-at = {2009-10-22 17:00:16}, priority = {2}, title = {Detection of a Biomarker for Alzheimer's Disease from Synthetic and Clinical Samples Using a Nanoscale Optical Biosensor}, url = {http://dx.doi.org/10.1021/ja044087q}, volume = {127}, year = {2005} }

TY - JOUR ID - haes_addl_05 L3 - citeulike-article-id:5985388 N2 - PMID: 15713105 A nanoscale optical biosensor based on localized surface plasmon resonance (LSPR) spectroscopy has been developed to monitor the interaction between the antigen, amyloid-beta derived diffusible ligands (ADDLs), and specific anti-ADDL antibodies. Using the sandwich assay format, this nanosensor provides quantitative binding information for both antigen and second antibody detection that permits the determination of ADDL concentration and offers the unique analysis of the aggregation mechanisms of this putative Alzheimer's disease pathogen at physiologically relevant monomer concentrations. Monitoring the LSPR-induced shifts from both ADDLs and a second polyclonal anti-ADDL antibody as a function of ADDL concentration reveals two ADDL epitopes that have binding constants to the specific anti-ADDL antibodies of 7.3 1012 M-1 and 9.5 108 M-1. The analysis of human brain extract and cerebrospinal fluid samples from control and Alzheimer's disease patients reveals that the LSPR nanosensor provides new information relevant to the understanding and possible diagnosis of Alzheimer's disease. IS - 7 JF - Journal of the American Chemical Society EP - 2271 TI - Detection of a Biomarker for Alzheimer's Disease from Synthetic and Clinical Samples Using a Nanoscale Optical Biosensor VL - 127 SP - 2264 KW - alzheimers KW - antibody KW - csf KW - lithography KW - lspr KW - nsl KW - protein KW - silver KW - spectroscopy AU - Haes, Amanda AU - Chang, Lei AU - Klein, William AU - Van Duyne, Richard PY - 2005/02/01/ UR - http://dx.doi.org/10.1021/ja044087q ER -