Effects of surface functional groups on protein adsorption and subsequent cell adhesion using self-assembled monolayers Export

@article{arima_tirf_07, abstract = {We investigated initial cell adhesion on self-assembled monolayers (SAMs) of alkanethiols carrying different functional groups including methyl (CH3), hydroxyl (OH), carboxylic acid (COOH), and amine (NH2). The combination of a surface plasmon resonance (SPR) instrument and a total internal reflection fluorescence microscope (TIRFM) allowed us to examine the kinetics of protein adsorption and correlating cell adhesion. Upon exposure of the SAM surface to a serum-containing medium, serum proteins rapidly adsorbed, and cells subsequently approached the surface. Adhesion of human umbilical vein endothelial cells (HUVECs) was greatly affected by surface functional groups; HUVECs adhered well to COOH- and NH2-SAMs, whereas poorly to CH3- and OH-SAMs. The amount of adsorbed protein from the serum-containing medium varied slightly with the terminal groups of the SAMs. On COOH- and NH2-SAMs, HUVECs adhered to bovine serum albumin (BSA)-preadsorbed surfaces with a few minutes delay, suggesting that displacement of preadsorbed BSA with cell-adhesive proteins, such as fibronectin or vitronectin, supports cell adhesion to these surfaces. Since the concentration of cell-adhesive proteins is much less than that of non-adhesive proteins such as BSA, displacement of adsorbed proteins with cell-adhesive proteins plays an important role in initial cell adhesion.}, author = {Arima, Yusuke and Iwata, Hiroo}, citeulike-article-id = {6054020}, citeulike-linkout-0 = {http://dx.doi.org/10.1039/b708099a}, citeulike-linkout-1 = {http://www.rsc.org/Publishing/Journals/article.asp?doi=b708099a}, doi = {10.1039/b708099a}, journal = {J. Mater. Chem.}, keywords = {bsa, cell, fluorescence, gold, sam, spr, thiol, tirf}, number = {38}, pages = {4079--4087}, posted-at = {2009-11-01 21:38:38}, priority = {2}, publisher = {The Royal Society of Chemistry}, title = {Effects of surface functional groups on protein adsorption and subsequent cell adhesion using self-assembled monolayers}, url = {http://dx.doi.org/10.1039/b708099a}, volume = {17}, year = {2007} }

TY - JOUR ID - arima_tirf_07 L3 - citeulike-article-id:6054020 N2 - We investigated initial cell adhesion on self-assembled monolayers (SAMs) of alkanethiols carrying different functional groups including methyl (CH3), hydroxyl (OH), carboxylic acid (COOH), and amine (NH2). The combination of a surface plasmon resonance (SPR) instrument and a total internal reflection fluorescence microscope (TIRFM) allowed us to examine the kinetics of protein adsorption and correlating cell adhesion. Upon exposure of the SAM surface to a serum-containing medium, serum proteins rapidly adsorbed, and cells subsequently approached the surface. Adhesion of human umbilical vein endothelial cells (HUVECs) was greatly affected by surface functional groups; HUVECs adhered well to COOH- and NH2-SAMs, whereas poorly to CH3- and OH-SAMs. The amount of adsorbed protein from the serum-containing medium varied slightly with the terminal groups of the SAMs. On COOH- and NH2-SAMs, HUVECs adhered to bovine serum albumin (BSA)-preadsorbed surfaces with a few minutes delay, suggesting that displacement of preadsorbed BSA with cell-adhesive proteins, such as fibronectin or vitronectin, supports cell adhesion to these surfaces. Since the concentration of cell-adhesive proteins is much less than that of non-adhesive proteins such as BSA, displacement of adsorbed proteins with cell-adhesive proteins plays an important role in initial cell adhesion. IS - 38 JF - J. Mater. Chem. EP - 4087 TI - Effects of surface functional groups on protein adsorption and subsequent cell adhesion using self-assembled monolayers PB - The Royal Society of Chemistry VL - 17 SP - 4079 KW - bsa KW - cell KW - fluorescence KW - gold KW - sam KW - spr KW - thiol KW - tirf AU - Arima, Yusuke AU - Iwata, Hiroo PY - 2007/// UR - http://dx.doi.org/10.1039/b708099a ER -