Pathways of Lipid Vesicle Deposition on Solid Surfaces: A Combined QCM-D and AFM Study Export

@article{richter_qcm_03, abstract = {Supported lipid bilayers (SLBs) are popular models of cell membranes with potential biotechnological applications, yet the mechanism of SLB formation is only partially understood. In this study, the adsorption and subsequent conformational changes of sonicated unilamellar vesicles on silica supports were investigated by quartz crystal microbalance with dissipation monitoring and atomic force microscopy, using mixtures of zwitterionic, negatively charged, and positively charged lipids, both in the presence and in the absence of Ca 2+ ions. Four different pathways of vesicle deposition could be distinguished. Depending on their charge, vesicles i), did not adsorb; ii), formed a stable vesicular layer; or iii), decomposed into an SLB after adsorption at high critical coverage or iv), at low coverage. Calcium was shown to enhance the tendency of SLB formation for negatively charged and zwitterionic vesicles. The role of vesicle-support, interbilayer, and intrabilayer interactions in the formation of SLBs is discussed.}, author = {Richter, Ralf and Mukhopadhyay, Anneke and Brisson, Alain}, citeulike-article-id = {6083070}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/S0006-3495(03)74722-5}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0006349503747225}, doi = {10.1016/S0006-3495(03)74722-5}, issn = {00063495}, journal = {Biophysical Journal}, keywords = {afm, bilayer, calcium, charged, fusion, kinetics, lipid, qcm, rupture, silicon, slb, vesicle}, month = {November}, number = {5}, pages = {3035--3047}, posted-at = {2009-11-07 16:05:18}, priority = {2}, title = {Pathways of Lipid Vesicle Deposition on Solid Surfaces: A Combined QCM-D and AFM Study}, url = {http://dx.doi.org/10.1016/S0006-3495(03)74722-5}, volume = {85}, year = {2003} }

TY - JOUR ID - richter_qcm_03 L3 - citeulike-article-id:6083070 N2 - Supported lipid bilayers (SLBs) are popular models of cell membranes with potential biotechnological applications, yet the mechanism of SLB formation is only partially understood. In this study, the adsorption and subsequent conformational changes of sonicated unilamellar vesicles on silica supports were investigated by quartz crystal microbalance with dissipation monitoring and atomic force microscopy, using mixtures of zwitterionic, negatively charged, and positively charged lipids, both in the presence and in the absence of Ca 2+ ions. Four different pathways of vesicle deposition could be distinguished. Depending on their charge, vesicles i), did not adsorb; ii), formed a stable vesicular layer; or iii), decomposed into an SLB after adsorption at high critical coverage or iv), at low coverage. Calcium was shown to enhance the tendency of SLB formation for negatively charged and zwitterionic vesicles. The role of vesicle-support, interbilayer, and intrabilayer interactions in the formation of SLBs is discussed. IS - 5 JF - Biophysical Journal SN - 00063495 EP - 3047 TI - Pathways of Lipid Vesicle Deposition on Solid Surfaces: A Combined QCM-D and AFM Study VL - 85 SP - 3035 KW - afm KW - bilayer KW - calcium KW - charged KW - fusion KW - kinetics KW - lipid KW - qcm KW - rupture KW - silicon KW - slb KW - vesicle AU - Richter, Ralf AU - Mukhopadhyay, Anneke AU - Brisson, Alain PY - 2003/11// UR - http://dx.doi.org/10.1016/S0006-3495(03)74722-5 ER -