Sensitive molecular binding assay using aphotonic crystal structure in total internalreflection Export

@article{citeulike:3203701, abstract = {A novel optical sensor for label-free biomolecular binding assay using a one-dimensional photonic crystal in a total-internal-reflection geometry is proposed and demonstrated. The simple configuration provides a narrow optical resonance to enable sensitive measurements of molecular binding, and at the same time employs an open interface to enable real-time measurements of binding dynamics. Ultrathin aminopropyltriethoxysilane/ glutaraldehyde films adsorbed on the interface were detected by measuring the spectral shift of the photonic crystal resonance and the intensity ratio change in a differential reflectance measurement. A detection limit of 6x10-5 nm for molecular layer thickness was obtained, which corresponds to a detection limit for analyte adsorption of 0.06 pg/mm2 or a refractive index resolution of 3x10-8 RIU; this represents a significant improvement relative to state-of-the-art surface-plasmon-resonance-based systems.}, author = {Guo, Yunbo and Divin, Charles and Myc, Andrzej and Terry and Baker and Norris, Theodore B. and Ye, Jing Y.}, citeulike-article-id = {3203701}, citeulike-linkout-0 = {http://dx.doi.org/10.1364/OE.16.011741}, citeulike-linkout-1 = {http://www.opticsinfobase.org/abstract.cfm?id=169943}, day = {4}, doi = {10.1364/OE.16.011741}, journal = {Opt. Express}, keywords = {biological-sensor, photonic-crystal-sensor, tir}, number = {16}, pages = {11741--11749}, posted-at = {2008-09-07 23:08:56}, priority = {2}, publisher = {OSA}, title = {Sensitive molecular binding assay using aphotonic crystal structure in total internalreflection}, url = {http://dx.doi.org/10.1364/OE.16.011741}, volume = {16}, year = {2008} }

TY - JOUR ID - citeulike:3203701 L3 - citeulike-article-id:3203701 N2 - A novel optical sensor for label-free biomolecular binding assay using a one-dimensional photonic crystal in a total-internal-reflection geometry is proposed and demonstrated. The simple configuration provides a narrow optical resonance to enable sensitive measurements of molecular binding, and at the same time employs an open interface to enable real-time measurements of binding dynamics. Ultrathin aminopropyltriethoxysilane/ glutaraldehyde films adsorbed on the interface were detected by measuring the spectral shift of the photonic crystal resonance and the intensity ratio change in a differential reflectance measurement. A detection limit of 6x10-5 nm for molecular layer thickness was obtained, which corresponds to a detection limit for analyte adsorption of 0.06 pg/mm2 or a refractive index resolution of 3x10-8 RIU; this represents a significant improvement relative to state-of-the-art surface-plasmon-resonance-based systems. IS - 16 JF - Opt. Express EP - 11749 TI - Sensitive molecular binding assay using aphotonic crystal structure in total internalreflection PB - OSA VL - 16 SP - 11741 KW - biological-sensor KW - photonic-crystal-sensor KW - tir AU - Guo, Yunbo AU - Divin, Charles AU - Myc, Andrzej AU - Terry AU - Baker AU - Norris, Theodore AU - Ye, Jing PY - 2008//04/ UR - http://dx.doi.org/10.1364/OE.16.011741 ER -