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Identification and Characterization of the “Super-Reduced” State of the H-Cluster in [FeFe] Hydrogenase: A New Building Block for the Catalytic Cycle?

by: Agnieszka Adamska, Alexey Silakov, Camilla Lambertz, Olaf Rüdiger, Thomas Happe, Edward Reijerse, Wolfgang Lubitz
Angew. Chem. Int. Ed., Vol. 51, No. 46. (12 November 2012), pp. 11458-11462, doi:10.1002/anie.201204800  Key: citeulike:11590510

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Abstract

Super-reduced and super-active: A new redox state in the active site of algal [FeFe] hydrogenases has been identified and characterized by EPR and FTIR spectroscopy. Electrochemical and in vitro essays show that this species is highly active in hydrogen production and suggest that it is a key intermediate in the catalytic cycle of all [FeFe] hydrogenases. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


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