Diagnosing platelet delta-storage pool disease in children by flow cytometry. Export

@article{citeulike:3091076, abstract = {Bleeding problems are symptomatic of platelet delta-storage pool diseases (SPDs) such as Hermansky-Pudlak syndrome. Although at present no cure is available for delta-SPD, early diagnosis is of great importance for prophylactic and supportive treatment. This study tested the usefulness of a flow cytometric assay for platelet serotonin in children. The assay was used to diagnose delta-SPD in a 10-year-old girl. Platelet serotonin levels were significantly lower in the patient than in all healthy control subjects (10 children and 10 adults). The serotonin results were supported by traditional tests, which are transmission electron microscopy of whole mounts and adenosine triphosphate release by lumi-aggregometry. The flow cytometric serotonin assay is a major improvement to current pediatric diagnostics. The advantages of this test are small sample volume of fresh or fixed/frozen platelets, availability of objective results within 2 hours of obtaining the blood sample, and automated analysis by flow cytometry.}, address = {Canadian Blood Services, University of British Columbia, Vancouver, Canada Centre for Blood Research, University of British Columbia, Vancouver, Canada.}, author = {Maurer-Spurej, E. and Pittendreigh, C. and Wu, J. K.}, citeulike-article-id = {3091076}, citeulike-linkout-0 = {http://dx.doi.org/10.1309/3KRYCPNAPDTVFWGY}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17369140}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17369140}, doi = {10.1309/3KRYCPNAPDTVFWGY}, issn = {0002-9173}, journal = {American journal of clinical pathology}, keywords = {5ht, delta, dense, flow, flowcytometry, serotonin, storagepool}, month = {April}, number = {4}, pages = {626--632}, posted-at = {2008-08-06 16:58:33}, priority = {2}, title = {Diagnosing platelet delta-storage pool disease in children by flow cytometry.}, url = {http://dx.doi.org/10.1309/3KRYCPNAPDTVFWGY}, volume = {127}, year = {2007} }

TY - JOUR ID - citeulike:3091076 L3 - citeulike-article-id:3091076 N2 - Bleeding problems are symptomatic of platelet delta-storage pool diseases (SPDs) such as Hermansky-Pudlak syndrome. Although at present no cure is available for delta-SPD, early diagnosis is of great importance for prophylactic and supportive treatment. This study tested the usefulness of a flow cytometric assay for platelet serotonin in children. The assay was used to diagnose delta-SPD in a 10-year-old girl. Platelet serotonin levels were significantly lower in the patient than in all healthy control subjects (10 children and 10 adults). The serotonin results were supported by traditional tests, which are transmission electron microscopy of whole mounts and adenosine triphosphate release by lumi-aggregometry. The flow cytometric serotonin assay is a major improvement to current pediatric diagnostics. The advantages of this test are small sample volume of fresh or fixed/frozen platelets, availability of objective results within 2 hours of obtaining the blood sample, and automated analysis by flow cytometry. IS - 4 JF - American journal of clinical pathology SN - 0002-9173 AD - Canadian Blood Services, University of British Columbia, Vancouver, Canada Centre for Blood Research, University of British Columbia, Vancouver, Canada. EP - 632 TI - Diagnosing platelet delta-storage pool disease in children by flow cytometry. VL - 127 SP - 626 KW - 5ht KW - delta KW - dense KW - flow KW - flowcytometry KW - serotonin KW - storagepool AU - Maurer-Spurej, E AU - Pittendreigh, C AU - Wu, JK PY - 2007/04// UR - http://dx.doi.org/10.1309/3KRYCPNAPDTVFWGY ER -