Evaluation of three automated chromogenic FVIII kits for the diagnosis of mild discrepant haemophilia A Export

@article{citeulike:4091427, abstract = {In some mild haemophilia A patients (discrepant phenotype), coagulation FVIII levels by one-stage assay (FVIII-1st) are more than double those by classical two-stage coagulation assay (FVIII-2st), and may fall within the normal range. Our aim was to assess automated two-stage chromogenic FVIII assays (FVIII-chr) for diagnosis of mild discrepant haemophilia A. Three chromogenic FVIII kits (Biophen, Coamatic and Dade-Behring) were evaluated, using recommended and extended incubation times. Samples were tested from patients with discrepant haemophilia (n = 7) and equivalent mild haemophilia (agreement between FVIII-1st and FVIII-2st, n = 4). For equivalent haemophilia, FVIII-chr were consistent with FVIII-1st and FVIII-2st for all kits at all incubation times. For discrepant haemophilia, using recommended incubation times, mean FVIII-chr using Biophen reagents was 22 IU/dl (range 13201331), with Coamatic 26 (17201334) and with Dade-Behring 41 (33201347), compared with 36 (27201344) for FVIII-1st and 8 (620139) for FVIII-2st. FVIII-chr decreased as incubation time was increased with Biophen and Coamatic, but decreased less with Dade-Behring. FVIII-chr using the Dade-Behring kit gave similar results to FVIII-1st and is not suitable for diagnosis of mild discrepant haemophilia A. FVIII-chr by Biophen and Coamatic kits is suitable for diagnosis of these patients, especially with an extended incubation time.}, address = {Haematology Division, Institute of Medical and Veterinary Science, Adelaide, SA, Australia}, author = {Rodgers, S. E. and Duncan, E. M. and Sobieraj-Teague, M. and Lloyd, J. V.}, citeulike-article-id = {4091427}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1751-553X.2007.01021.x}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/120175050/ABSTRACT}, doi = {10.1111/j.1751-553X.2007.01021.x}, journal = {International Journal of Laboratory Hematology}, keywords = {assay, assays, automated, chromogenic, discrepant, evaluation, fviii, onestage, twostage}, number = {2}, pages = {180--188}, posted-at = {2009-02-24 10:05:44}, priority = {2}, title = {Evaluation of three automated chromogenic FVIII kits for the diagnosis of mild discrepant haemophilia A}, url = {http://dx.doi.org/10.1111/j.1751-553X.2007.01021.x}, volume = {31}, year = {2009} }

TY - JOUR ID - citeulike:4091427 L3 - citeulike-article-id:4091427 N2 - In some mild haemophilia A patients (discrepant phenotype), coagulation FVIII levels by one-stage assay (FVIII-1st) are more than double those by classical two-stage coagulation assay (FVIII-2st), and may fall within the normal range. Our aim was to assess automated two-stage chromogenic FVIII assays (FVIII-chr) for diagnosis of mild discrepant haemophilia A. Three chromogenic FVIII kits (Biophen, Coamatic and Dade-Behring) were evaluated, using recommended and extended incubation times. Samples were tested from patients with discrepant haemophilia (n = 7) and equivalent mild haemophilia (agreement between FVIII-1st and FVIII-2st, n = 4). For equivalent haemophilia, FVIII-chr were consistent with FVIII-1st and FVIII-2st for all kits at all incubation times. For discrepant haemophilia, using recommended incubation times, mean FVIII-chr using Biophen reagents was 22 IU/dl (range 13201331), with Coamatic 26 (17201334) and with Dade-Behring 41 (33201347), compared with 36 (27201344) for FVIII-1st and 8 (620139) for FVIII-2st. FVIII-chr decreased as incubation time was increased with Biophen and Coamatic, but decreased less with Dade-Behring. FVIII-chr using the Dade-Behring kit gave similar results to FVIII-1st and is not suitable for diagnosis of mild discrepant haemophilia A. FVIII-chr by Biophen and Coamatic kits is suitable for diagnosis of these patients, especially with an extended incubation time. IS - 2 JF - International Journal of Laboratory Hematology AD - Haematology Division, Institute of Medical and Veterinary Science, Adelaide, SA, Australia EP - 188 TI - Evaluation of three automated chromogenic FVIII kits for the diagnosis of mild discrepant haemophilia A VL - 31 SP - 180 KW - assay KW - assays KW - automated KW - chromogenic KW - discrepant KW - evaluation KW - fviii KW - onestage KW - twostage AU - Rodgers, SE AU - Duncan, EM AU - Sobieraj-Teague, M AU - Lloyd, JV PY - 2009/// UR - http://dx.doi.org/10.1111/j.1751-553X.2007.01021.x ER -