Diagnostic utility of light transmission platelet aggregometry: results from a prospective study of individuals referred for bleeding disorder assessments Export

@article{citeulike:4214802, abstract = {See also Mumford AD. How useful is light transmission platelet aggregometry for the diagnosis of bleeding disorders? This issue, pp 67320135.Summary.  Background: Light transmission aggregometry (LTA) is commonly performed to assess individuals for bleeding disorders. Objectives: The goal was to evaluate the incidence and spectrum of platelet function abnormalities in a prospective cohort of individuals referred for bleeding disorder assessments after exclusion of thrombocytopenia and von Willebrand disease. Patients/methods: Subjects were healthy controls and patients from a prospective cohort of individuals referred for bleeding disorder assessments after exclusion of thrombocytopenia and von Willebrand disease. LTA was performed by standardized methods using platelet-rich plasma adjusted to 250 נ109 platelets L22121. Maximal aggregation data were analyzed to determine the likelihood of detecting a platelet function disorder by LTA, and the sensitivity and specificity of LTA for platelet disorders. Results: The incidence of false positive LTA among subjects excluded of having bleeding disorders was similar to healthy controls. Abnormal LTA was more common in subjects with bleeding disorders and the likelihood of a bleeding disorder was significantly increased (odds ratio 32) when maximal aggregation was reduced with two or more agonists. Receiver operator curve analyses indicated that LTA had high specificity and moderate sensitivity for detecting inherited defects in platelet function and that the LTA agonists 1.25 03BCg mL22121 collagen, 6 03BCM epinephrine, 1.6 mM arachidonic acid and 1.0 03BCM thromboxane analogue U44619 detected most inherited disorders with abnormal LTA. Conclusions: LTA is valuable for detecting platelet function abnormalities among individuals referred for bleeding problems, particularly when the test indicates abnormal responses to multiple agonists.}, address = {Department of Pathology and Molecular Medicine; Department of Medicine, McMaster University, Hamilton, ON; The Hamilton Regional Laboratory Medicine Program, Hamilton, ON; Department of Statistics and Actuarial Science, University of Waterloo, Waterloo, ON, Canada}, author = {Hayward, C. P. M. and Pai, M. and Liu, Y. and Moffat, K. A. and Seecharan, J. and Webert, K. E. and Cook, R. J. and Heddle, N. M.}, citeulike-article-id = {4214802}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1538-7836.2009.03273.x}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19143930}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19143930}, citeulike-linkout-3 = {http://www3.interscience.wiley.com/cgi-bin/abstract/121627733/ABSTRACT}, doi = {10.1111/j.1538-7836.2009.03273.x}, journal = {Journal of Thrombosis and Haemostasis}, keywords = {aggregation, agonists, lta, method, methods, review}, number = {4}, pages = {676--684}, posted-at = {2009-03-24 16:17:31}, priority = {5}, title = {Diagnostic utility of light transmission platelet aggregometry: results from a prospective study of individuals referred for bleeding disorder assessments}, url = {http://dx.doi.org/10.1111/j.1538-7836.2009.03273.x}, volume = {7}, year = {2009} }

TY - JOUR ID - citeulike:4214802 L3 - citeulike-article-id:4214802 N2 - See also Mumford AD. How useful is light transmission platelet aggregometry for the diagnosis of bleeding disorders? This issue, pp 67320135.Summary.  Background: Light transmission aggregometry (LTA) is commonly performed to assess individuals for bleeding disorders. Objectives: The goal was to evaluate the incidence and spectrum of platelet function abnormalities in a prospective cohort of individuals referred for bleeding disorder assessments after exclusion of thrombocytopenia and von Willebrand disease. Patients/methods: Subjects were healthy controls and patients from a prospective cohort of individuals referred for bleeding disorder assessments after exclusion of thrombocytopenia and von Willebrand disease. LTA was performed by standardized methods using platelet-rich plasma adjusted to 250 נ109 platelets L22121. Maximal aggregation data were analyzed to determine the likelihood of detecting a platelet function disorder by LTA, and the sensitivity and specificity of LTA for platelet disorders. Results: The incidence of false positive LTA among subjects excluded of having bleeding disorders was similar to healthy controls. Abnormal LTA was more common in subjects with bleeding disorders and the likelihood of a bleeding disorder was significantly increased (odds ratio 32) when maximal aggregation was reduced with two or more agonists. Receiver operator curve analyses indicated that LTA had high specificity and moderate sensitivity for detecting inherited defects in platelet function and that the LTA agonists 1.25 03BCg mL22121 collagen, 6 03BCM epinephrine, 1.6 mM arachidonic acid and 1.0 03BCM thromboxane analogue U44619 detected most inherited disorders with abnormal LTA. Conclusions: LTA is valuable for detecting platelet function abnormalities among individuals referred for bleeding problems, particularly when the test indicates abnormal responses to multiple agonists. IS - 4 JF - Journal of Thrombosis and Haemostasis AD - Department of Pathology and Molecular Medicine; Department of Medicine, McMaster University, Hamilton, ON; The Hamilton Regional Laboratory Medicine Program, Hamilton, ON; Department of Statistics and Actuarial Science, University of Waterloo, Waterloo, ON, Canada EP - 684 TI - Diagnostic utility of light transmission platelet aggregometry: results from a prospective study of individuals referred for bleeding disorder assessments VL - 7 SP - 676 KW - aggregation KW - agonists KW - lta KW - method KW - methods KW - review AU - Hayward, CPM AU - Pai, M AU - Liu, Y AU - Moffat, KA AU - Seecharan, J AU - Webert, KE AU - Cook, RJ AU - Heddle, NM PY - 2009/// UR - http://dx.doi.org/10.1111/j.1538-7836.2009.03273.x ER -