Diagnosis of mild platelet function disorders. Reliability and usefulness of light transmission platelet aggregation and serotonin secretion assays Export

@article{citeulike:5828478, abstract = {Light transmission platelet aggregation (PA), adapted to measure platelet secretion (PS), is the reference test for diagnosing platelet functional disorders (PFD). Problems with these assays include lack of standardisation, unknown reproducibility and lack of universally accepted diagnostic criteria. We addressed these issues in patients with inherited mucocutaneous bleeding (MCB). Normal and abnormal PA tests in 213 patients were reproducible in 93·3\% and 90·4\% of the cases, respectively. Mean intra-subject coefficient of variation for PA with strong agonists were \<9\% and mean intra-class correlation coefficient for weak agonists were \>0·86 (P \< 0·0001). Concomitant impaired PA with 10 03BCmol/l-adrenaline and 4 03BCmol/l-ADP was observed in 13·7\% of the controls. This combination was not considered per se a criterion for PFD. PA with adrenaline 226542\% or irreversible aggregation with 4 03BCmol/l ADP had 93\% and 95\% Negative Predictive Value for diagnosing PFD, respectively. PA defects were consistently associated with abnormal PS. In contrast, 14·3\% of patients with MCB had isolated PS. Thus, standardized PA/PS assays are highly reproducible and concordant in normal and patient populations. Normal PA with adrenaline and low ADP concentration robustly predict a normal PA. Simultaneous PA/PS assays enable the diagnosis of isolated PS defects. This study confirmed that hereditary PA2013PS defects are highly prevalent.}, address = {Departments of Clinical Laboratory; Haematology-Oncology; Paediatrics, School of Medicine; Department of Cell and Molecular Biology, Faculty of Biological Sciences, Pontificia Universidad Catlica de Chile, Santiago, Chile}, author = {Quiroga, Teresa and Goycoolea, Manuela and Matus, Valeria and Z\'{u}\~{n}iga, Pamela and Mart\'{\i}nez, Carlos and Garrido, Marcelo and Aranda, Eduardo and Leighton, Federico and Panes, Olga and Pereira, Jaime and Mezzano, Diego}, citeulike-article-id = {5828478}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1365-2141.2009.07890.x}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/122604927/ABSTRACT}, doi = {10.1111/j.1365-2141.2009.07890.x}, issn = {1365-2141}, journal = {British Journal of Haematology}, keywords = {aggregation, assay, assays, lta, platelet, platelet\_aggregation, platelet\_function, platelets, serotonin}, number = {5}, pages = {729--736}, posted-at = {2009-09-23 08:52:40}, priority = {5}, title = {Diagnosis of mild platelet function disorders. Reliability and usefulness of light transmission platelet aggregation and serotonin secretion assays}, url = {http://dx.doi.org/10.1111/j.1365-2141.2009.07890.x}, volume = {147}, year = {2009} }

TY - JOUR ID - citeulike:5828478 L3 - citeulike-article-id:5828478 N2 - Light transmission platelet aggregation (PA), adapted to measure platelet secretion (PS), is the reference test for diagnosing platelet functional disorders (PFD). Problems with these assays include lack of standardisation, unknown reproducibility and lack of universally accepted diagnostic criteria. We addressed these issues in patients with inherited mucocutaneous bleeding (MCB). Normal and abnormal PA tests in 213 patients were reproducible in 93·3% and 90·4% of the cases, respectively. Mean intra-subject coefficient of variation for PA with strong agonists were <9% and mean intra-class correlation coefficient for weak agonists were >0·86 (P < 0·0001). Concomitant impaired PA with 10 03BCmol/l-adrenaline and 4 03BCmol/l-ADP was observed in 13·7% of the controls. This combination was not considered per se a criterion for PFD. PA with adrenaline 226542% or irreversible aggregation with 4 03BCmol/l ADP had 93% and 95% Negative Predictive Value for diagnosing PFD, respectively. PA defects were consistently associated with abnormal PS. In contrast, 14·3% of patients with MCB had isolated PS. Thus, standardized PA/PS assays are highly reproducible and concordant in normal and patient populations. Normal PA with adrenaline and low ADP concentration robustly predict a normal PA. Simultaneous PA/PS assays enable the diagnosis of isolated PS defects. This study confirmed that hereditary PA2013PS defects are highly prevalent. IS - 5 JF - British Journal of Haematology SN - 1365-2141 AD - Departments of Clinical Laboratory; Haematology-Oncology; Paediatrics, School of Medicine; Department of Cell and Molecular Biology, Faculty of Biological Sciences, Pontificia Universidad Catlica de Chile, Santiago, Chile EP - 736 TI - Diagnosis of mild platelet function disorders. Reliability and usefulness of light transmission platelet aggregation and serotonin secretion assays VL - 147 SP - 729 KW - aggregation KW - assay KW - assays KW - lta KW - platelet KW - platelet_aggregation KW - platelet_function KW - platelets KW - serotonin AU - Quiroga, Teresa AU - Goycoolea, Manuela AU - Matus, Valeria AU - Zúñiga, Pamela AU - Martínez, Carlos AU - Garrido, Marcelo AU - Aranda, Eduardo AU - Leighton, Federico AU - Panes, Olga AU - Pereira, Jaime AU - Mezzano, Diego PY - 2009/// UR - http://dx.doi.org/10.1111/j.1365-2141.2009.07890.x ER -