Circular dichroism and conformational polymorphism of DNA Export

@article{citeulike:4006434, abstract = {Here we review studies that provided important information about conformational properties of DNA using circular dichroic (CD) spectroscopy. The conformational properties include the B-family of structures, A-form, Z-form, guanine quadruplexes, cytosine quadruplexes, triplexes and other less characterized structures. CD spectroscopy is extremely sensitive and relatively inexpensive. This fast and simple method can be used at low- as well as high-DNA concentrations and with short- as well as long-DNA molecules. The samples can easily be titrated with various agents to cause conformational isomerizations of DNA. The course of detected CD spectral changes makes possible to distinguish between gradual changes within a single DNA conformation and cooperative isomerizations between discrete structural states. It enables measuring kinetics of the appearance of particular conformers and determination of their thermodynamic parameters. In careful hands, CD spectroscopy is a valuable tool for mapping conformational properties of particular DNA molecules. Due to its numerous advantages, CD spectroscopy significantly participated in all basic conformational findings on DNA. 10.1093/nar/gkp026}, author = {Kypr, Jaroslav and Kejnovska, Iva and Renciuk, Daniel and Vorlickova, Michaela}, citeulike-article-id = {4006434}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/nar/gkp026}, citeulike-linkout-1 = {http://nar.oxfordjournals.org/content/37/6/1713.abstract}, citeulike-linkout-2 = {http://nar.oxfordjournals.org/content/37/6/1713.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19190094}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19190094}, day = {1}, doi = {10.1093/nar/gkp026}, journal = {Nucl. Acids Res.}, keywords = {dna-ab-exp, dna-forms}, month = {April}, number = {6}, pages = {1713--1725}, posted-at = {2009-10-09 11:06:24}, priority = {2}, title = {Circular dichroism and conformational polymorphism of DNA}, url = {http://dx.doi.org/10.1093/nar/gkp026}, volume = {37}, year = {2009} }

TY - JOUR ID - citeulike:4006434 L3 - citeulike-article-id:4006434 N2 - Here we review studies that provided important information about conformational properties of DNA using circular dichroic (CD) spectroscopy. The conformational properties include the B-family of structures, A-form, Z-form, guanine quadruplexes, cytosine quadruplexes, triplexes and other less characterized structures. CD spectroscopy is extremely sensitive and relatively inexpensive. This fast and simple method can be used at low- as well as high-DNA concentrations and with short- as well as long-DNA molecules. The samples can easily be titrated with various agents to cause conformational isomerizations of DNA. The course of detected CD spectral changes makes possible to distinguish between gradual changes within a single DNA conformation and cooperative isomerizations between discrete structural states. It enables measuring kinetics of the appearance of particular conformers and determination of their thermodynamic parameters. In careful hands, CD spectroscopy is a valuable tool for mapping conformational properties of particular DNA molecules. Due to its numerous advantages, CD spectroscopy significantly participated in all basic conformational findings on DNA. 10.1093/nar/gkp026 IS - 6 JF - Nucl. Acids Res. EP - 1725 TI - Circular dichroism and conformational polymorphism of DNA VL - 37 SP - 1713 KW - dna-ab-exp KW - dna-forms AU - Kypr, Jaroslav AU - Kejnovska, Iva AU - Renciuk, Daniel AU - Vorlickova, Michaela PY - 2009/04/01/ UR - http://dx.doi.org/10.1093/nar/gkp026 ER -