Aromatization of androgens by human abdominal and breast fat tissue. Export

@article{citeulike:4221883, abstract = {The ability of human abdominal, breast and axillary fat to convert androgens into estrogens was investigated by incubating labeled substrates in the presence of NADPH with a variety of cell preparations. The incubation products were subjected to phenolic partition, paper chromatography, methyl-ether formation, repeat chromatography and crystallization with cold carrier reference standards to constant specific activity. Androstenedione was converted to estrone and, to a lesser extent, to 17beta-estradiol by crude homogenates, minces, fat-free particulate fractions (1,000-100,000 time g) and isolated fat cells obtained from abdominal, breast or axillary fat. Testosterone was found to be aromatized as actively as androstenedione, but inthis case more 17 beta-estrodiol was formed than estrone. 19-Hydroxyandrostenedione-2 also served as substrate, givingresults similar to those obtained with androstenedione. Fat tissue obtained from cancerous breasts was found to be as active as normal breast fat (1-4 pg/g fat/90 min) and within the range found for abdominal fat (1-27 pg/g fat/90 min). In each case in which axillary fat was compared to breast fat from the same subject, the activity of the axillary fat was 5 to 10 times higher. The results indicate a possible role of adipose tissue as a significant extra-gonadal source of estrogens.}, author = {Nimrod, A. and Ryan, K. J.}, citeulike-article-id = {4221883}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/234975}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=234975}, issn = {0021-972X}, journal = {The Journal of clinical endocrinology and metabolism}, keywords = {abdominal, adipose, aromatase, breast, human}, month = {March}, number = {3}, pages = {367--372}, posted-at = {2009-03-26 10:53:33}, priority = {2}, title = {Aromatization of androgens by human abdominal and breast fat tissue.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/234975}, volume = {40}, year = {1975} }

TY - JOUR ID - citeulike:4221883 L3 - citeulike-article-id:4221883 N2 - The ability of human abdominal, breast and axillary fat to convert androgens into estrogens was investigated by incubating labeled substrates in the presence of NADPH with a variety of cell preparations. The incubation products were subjected to phenolic partition, paper chromatography, methyl-ether formation, repeat chromatography and crystallization with cold carrier reference standards to constant specific activity. Androstenedione was converted to estrone and, to a lesser extent, to 17beta-estradiol by crude homogenates, minces, fat-free particulate fractions (1,000-100,000 time g) and isolated fat cells obtained from abdominal, breast or axillary fat. Testosterone was found to be aromatized as actively as androstenedione, but inthis case more 17 beta-estrodiol was formed than estrone. 19-Hydroxyandrostenedione-2 also served as substrate, givingresults similar to those obtained with androstenedione. Fat tissue obtained from cancerous breasts was found to be as active as normal breast fat (1-4 pg/g fat/90 min) and within the range found for abdominal fat (1-27 pg/g fat/90 min). In each case in which axillary fat was compared to breast fat from the same subject, the activity of the axillary fat was 5 to 10 times higher. The results indicate a possible role of adipose tissue as a significant extra-gonadal source of estrogens. IS - 3 JF - The Journal of clinical endocrinology and metabolism SN - 0021-972X EP - 372 TI - Aromatization of androgens by human abdominal and breast fat tissue. VL - 40 SP - 367 KW - abdominal KW - adipose KW - aromatase KW - breast KW - human AU - Nimrod, A AU - Ryan, KJ PY - 1975/03// UR - http://view.ncbi.nlm.nih.gov/pubmed/234975 ER -