Effects of androgens on adipocyte differentiation and adipose tissue explant metabolism in men and women. Export

@article{citeulike:4880102, abstract = {SUMMARY Objective: To examine the effects of aromatizable or non-aromatizable androgens on abdominal subcutaneous (SC) and omental (OM) adipose tissue lipid metabolism and adipogenesis in men and women. Design and subjects: Primary organ and preadipocyte cultures were established from surgical samples obtained in men (n=22) and women undergoing biliopancreatic diversions (n=12) or gynecological surgeries (n=8). Cultures were treated with testosterone, dihydrotestosterone (DHT) and methyltrienolone (R1881). Measurements: Heparin-releasable lipoprotein lipase (HR-LPL) activity, glycerol release, adiponectin secretion, glycerol-3-phosphate dehydrogenase activity and lipid accumulation were measured. Results: In organ cultures from men, DHT had a statistically significant inhibitory effect on HR-LPL activity in the OM compartment. Testosterone significantly inhibited HR-LPL activity in SC and OM cultures. In women, high DHT concentrations tended to inhibit HR-LPL activity in OM cultures. Minor androgenic effects were observed for basal and isoproterenol-stimulated lipolysis as well as adiponectin release in men. On the other hand, adipocyte differentiation was significantly and dose-dependently inhibited by DHT, testosterone and R1881 in SC and OM cultures from both sexes. These effects did not differ according to adipose tissue depot but appeared to be more pronounced in women than in men. Conclusions: Androgens slightly decreased HR-LPL activity in adipose tissue organ cultures, but markedly inhibited adipogenesis in SC and OM primary preadipocyte cultures in both sexes. Androgenic effects on adipose tissue in men vs. women may not differ in terms of direction but in the magnitude of their negative impact on adipogenesis and lipid synthesis.}, author = {Blouin, Karine and Nadeau, M\'{e}lanie and Perreault, Martin and Veilleux, Alain and Drolet, Ren\'{e}e and Marceau, Picard and Mailloux, Jacques and Luu-The, Van and Tchernof, Andr\'{e}}, citeulike-article-id = {4880102}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1365-2265.2009.03645.x}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19500113}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19500113}, day = {2}, doi = {10.1111/j.1365-2265.2009.03645.x}, issn = {1365-2265}, journal = {Clinical endocrinology}, keywords = {adipocyte, adipose, androgens, differentiation, human, men, women}, month = {June}, posted-at = {2009-06-17 13:39:15}, priority = {2}, title = {Effects of androgens on adipocyte differentiation and adipose tissue explant metabolism in men and women.}, url = {http://dx.doi.org/10.1111/j.1365-2265.2009.03645.x}, year = {2009} }

TY - JOUR ID - citeulike:4880102 L3 - citeulike-article-id:4880102 N2 - SUMMARY Objective: To examine the effects of aromatizable or non-aromatizable androgens on abdominal subcutaneous (SC) and omental (OM) adipose tissue lipid metabolism and adipogenesis in men and women. Design and subjects: Primary organ and preadipocyte cultures were established from surgical samples obtained in men (n=22) and women undergoing biliopancreatic diversions (n=12) or gynecological surgeries (n=8). Cultures were treated with testosterone, dihydrotestosterone (DHT) and methyltrienolone (R1881). Measurements: Heparin-releasable lipoprotein lipase (HR-LPL) activity, glycerol release, adiponectin secretion, glycerol-3-phosphate dehydrogenase activity and lipid accumulation were measured. Results: In organ cultures from men, DHT had a statistically significant inhibitory effect on HR-LPL activity in the OM compartment. Testosterone significantly inhibited HR-LPL activity in SC and OM cultures. In women, high DHT concentrations tended to inhibit HR-LPL activity in OM cultures. Minor androgenic effects were observed for basal and isoproterenol-stimulated lipolysis as well as adiponectin release in men. On the other hand, adipocyte differentiation was significantly and dose-dependently inhibited by DHT, testosterone and R1881 in SC and OM cultures from both sexes. These effects did not differ according to adipose tissue depot but appeared to be more pronounced in women than in men. Conclusions: Androgens slightly decreased HR-LPL activity in adipose tissue organ cultures, but markedly inhibited adipogenesis in SC and OM primary preadipocyte cultures in both sexes. Androgenic effects on adipose tissue in men vs. women may not differ in terms of direction but in the magnitude of their negative impact on adipogenesis and lipid synthesis. JF - Clinical endocrinology SN - 1365-2265 TI - Effects of androgens on adipocyte differentiation and adipose tissue explant metabolism in men and women. KW - adipocyte KW - adipose KW - androgens KW - differentiation KW - human KW - men KW - women AU - Blouin, Karine AU - Nadeau, Mélanie AU - Perreault, Martin AU - Veilleux, Alain AU - Drolet, Renée AU - Marceau, Picard AU - Mailloux, Jacques AU - Luu-The, Van AU - Tchernof, André PY - 2009/06/02/ UR - http://dx.doi.org/10.1111/j.1365-2265.2009.03645.x ER -