Effects of dihydrotestosterone on skeletal muscle transcriptome in mice measured by serial analysis of gene expression Export

@article{citeulike:6041080, abstract = {In order to characterize the action of androgen in skeletal muscle, we have investigated the effects of castration (GDX) and dihydrotestosterone (DHT) on global gene expression in mice. The serial analysis of gene expression method was performed in the muscle of male mice in six experimental groups: intact, GDX and GDX+DHT injection 1, 3, 6 or 24 h before they were killed. A total of 780 822 sequenced tags quantified the expression level of 80 142 tag species. Thirteen and seventy-nine transcripts were differentially expressed in GDX and DHT respectively (P < 0.05), including eight partially characterized and 21 potential novel transcripts. The induced transcripts within 3 h after DHT injection were involved in the following functions: transcription, protein synthesis, modification and degradation, muscle contraction and relaxation, cell signaling, polyamine biosynthesis, cell cycle progression and arrest, angiogenesis, energy metabolism and immunity. However, the inductions of transcripts related to cell cycle arrest and angiogenesis were no longer significant 24 h after DHT injection. The current study might suggest that DHT promotes protein synthesis, cell signaling, cell proliferation and ATP production, as well as muscle contraction and relaxation at the transcriptional level in skeletal muscle in vivo. 10.1677/jme.1.01964}, author = {Yoshioka, M. and Boivin, A. and Ye, P. and Labrie, F. and St-Amand, J.}, citeulike-article-id = {6041080}, citeulike-linkout-0 = {http://dx.doi.org/10.1677/jme.1.01964}, citeulike-linkout-1 = {http://jme.endocrinology-journals.org/content/36/2/247.abstract}, citeulike-linkout-2 = {http://jme.endocrinology-journals.org/content/36/2/247.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/16595697}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=16595697}, day = {1}, doi = {10.1677/jme.1.01964}, journal = {J Mol Endocrinol}, keywords = {androgen, dht, expression, mice, transcript}, month = {April}, number = {2}, pages = {247--259}, posted-at = {2009-10-30 10:02:59}, priority = {2}, title = {Effects of dihydrotestosterone on skeletal muscle transcriptome in mice measured by serial analysis of gene expression}, url = {http://dx.doi.org/10.1677/jme.1.01964}, volume = {36}, year = {2006} }

TY - JOUR ID - citeulike:6041080 L3 - citeulike-article-id:6041080 N2 - In order to characterize the action of androgen in skeletal muscle, we have investigated the effects of castration (GDX) and dihydrotestosterone (DHT) on global gene expression in mice. The serial analysis of gene expression method was performed in the muscle of male mice in six experimental groups: intact, GDX and GDX+DHT injection 1, 3, 6 or 24 h before they were killed. A total of 780 822 sequenced tags quantified the expression level of 80 142 tag species. Thirteen and seventy-nine transcripts were differentially expressed in GDX and DHT respectively (P < 0.05), including eight partially characterized and 21 potential novel transcripts. The induced transcripts within 3 h after DHT injection were involved in the following functions: transcription, protein synthesis, modification and degradation, muscle contraction and relaxation, cell signaling, polyamine biosynthesis, cell cycle progression and arrest, angiogenesis, energy metabolism and immunity. However, the inductions of transcripts related to cell cycle arrest and angiogenesis were no longer significant 24 h after DHT injection. The current study might suggest that DHT promotes protein synthesis, cell signaling, cell proliferation and ATP production, as well as muscle contraction and relaxation at the transcriptional level in skeletal muscle in vivo. 10.1677/jme.1.01964 IS - 2 JF - J Mol Endocrinol EP - 259 TI - Effects of dihydrotestosterone on skeletal muscle transcriptome in mice measured by serial analysis of gene expression VL - 36 SP - 247 KW - androgen KW - dht KW - expression KW - mice KW - transcript AU - Yoshioka, M AU - Boivin, A AU - Ye, P AU - Labrie, F AU - St-Amand, J PY - 2006/04/01/ UR - http://dx.doi.org/10.1677/jme.1.01964 ER -